Page 179 - Acrobat J Trad-21-3-2566

662 วารสารการแพทย์แผนไทยและการแพทย์ ทางเลือก ปีที่ 21 ฉบับที่ 3 กันยายน-ธันวาคม 2566

ฟลาโวนอยด์ ซึ่งมีความส�าคัญต่อความสามารถในการต้านอนุมูลอิสระและต้านการอักเสบ
ข้อสรุปและข้อเสนอแนะ: จากการศึกษาครั้งนี้ท�าให้สรุปได้ว่าต�ารับยาแก้ลมอุทธังคมาวาตาเป็นต�ารับยาที่มี
ฤทธิ์ต้านอนุมูลอิสระและต้านการอักเสบ จึงควรมีการศึกษาอื่น ๆ เพิ่มเติม เพื่อศึกษาความเป็นไปได้ในการน�าไปใช้
รักษาข้อเข่าเสื่่อมต่อไป

ค�ำส�ำคัญ: ต�ารับยาแก้ลมอุทธังคมาวาตา, ฤทธิ์ต้านอนุมูลอิสระ, ฤทธิ์ต้านการอักเสบ
Antioxidant and Nitric Oxide Inhibitory Activities of Ya-Kae-Lom-Utthang-
Ka-Ma-Va-Ta Thai Traditional Medicine Formula in ATDC-5 Cells

Waluga Plaingam , Somporn Phonkrathok , Sanhajutha Puangmala , Moragot Chatatikun †,‡
*
*
*
College of Oriental Medicine, Rangsit University, Lak Hok Sub-District, Mueang Pathum Thani District, Pathum
*
Thani 12000, Thailand.
Department of Medical Technology, School of Allied Health Sciences, Walailak University, Thai Buri Sub-District,
†
Tha Sala District, Nakhon Si Thammarat 80160, Thailand.
‡ Corresponding author: moragot.ch@wu.ac.th
Abstract

Introduction and Objectives: Knee osteoarthritis (KOA), a disabling joint inflammatory disease, is
caused by nitric oxide (NO) established as one of its major causes. Many previous studies have shown that bioac-
tive compounds and plant extracts can exhibit chondroprotective effects by suppressing the expression of NO and
inducible nitric oxide synthase (iNOS). The objectives of this research were to determine total phenolic content and
total flavonoid content, and investigate antioxidant and inhibitory effects of nitric oxide by 95% ethanolic extract
of Ya-Kae-Lom-Utthang-Ka-Ma-Va-Ta (UKLU) formula in ADTC-5 mouse chondrogenic cells.
Methods: The total phenolic and total flavonoid contents were determined by folin ciocalteu and aluminium
chloride methods, respectively. Antioxidant activity was also determined using DPPH and ABTS radical scaveng-
ing assays. Inhibitory activity against nitric oxide production was induced by lipopolysaccharide (LPS) used as the
assay for anti-inflammation in ATDC-5 cells. In finally, the MTT assay was used to measure cytotoxicity.
Results: In the UKLU formula extract, 479.33 ± 2.08 mg GAE/g and 177.89 ± 3.02 mg GAE/g of total
phenolics and total flavonoids, respectively, were found. In the formula extract’s antioxidant activity testing, the
IC 50 values were 137.72 µg/mL for DPPH method and 27.28 µg/mL in ABTS method. And finally, the formula
extract was further tested for anti-inflammatory via inhibition of nitric oxide production and cytotoxicity in ATDC-5
cells. The ethanolic extract of the formula showed the nitric oxide inhibitory activities with the IC 50 value at 41.88
µg/mL.
Discussion: This study provides scientific information on YKLU formula’s antioxidant and anti-inflammatory
activities via NO inhibition effects, which are associated with KOA. The effects might mainly result from phenolic
compounds and flavonoids with such antioxidant and anti-inflammatory capacities.
Conclusion and Recommendation: The present investigation indicates that the YKLU formula has
antioxidant and anti-inflammatory activities. Further studies should be carried out on the feasibility of using the
formula in treating KOA in the future.
Key words: Ya-Kae-Lom-Utthang-Ka-Ma-Va-Ta, antioxidant activity, anti-inflammatory