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Abstract
Chemical Constituents of Nelumbo nucifera Gaertn Leaf Extract Exhibiting Hypolipidemia
Activity
Somchit Niumsakul*, Duangpen Pattamadilok*, Nanteetip Limpeanchob**, Kornkanok Ingkaninan**,
Prapai Wongsinkongman*, Sununta Srisopon*
*Medicinal Plant Research Institute, Department of Medical Sciences, Nonthaburi 11000
**Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000
The purpose of this study was to investigate and isolate compounds from ethanolic extract of lotus leaves
(Nelumbo nucifera Gaertn) exhibiting inhibitory activity against pancreatic lipase or HMG-CoA reductase.
From a previous study, it was found that the partially purified portion of the ethanolic extract of lotus leaves
(Nn-E3) showed the best inhibitory activity against pancreatic lipase enzyme, with IC 50 at 36.80 μg/ml, using
orlistat as a positive control. In addition, the crude extract at the concentration of 10 μg/ml showed moderate
inhibitory activity (65.87%) against HMG-CoA reductase, using pravastatin as a positive control. Therefore,
it was interesting to further isolate the constituents from Nn-E3. As a result, two flavonoid compounds (A, B)
were purified using column chromatography and their chemical structures were elucidated using NMR and
MS spectroscopic techniques. These compounds were identified as quercetin 3-O-β-D-glucuronide (miquelianin)
and rutin respectively.
Key words: Nelumbo nucifera Gaertn. leaf, miquelianin, quercetin 3-O-β-D-glucuronide, rutin