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o Journal of Thai Traditional & Alternative Medicine
Journal of Thai Traditional & Alternative Medicine Vol. 8 Nos. 2-3 May-December 2010 Ò˜ı
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μ“√“ß∑’Ë Ú H and C (125 MHz) NMR spectral data of rutin and H, C (500 MHz) of “√ B (in DMSO-d )
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a
Rutin Compound B
Position
1 H (mult., J in Hz) 13 C 1 H (mult., J in Hz) 13 C
2 - 156.4 - 156.2
3 - 133.3 - 133.1
4 - 177.4 - 177.0
5 - 161.2 - 160.9
6 6.20 (d, 2.1) 98.7 6.19 (br s) 98.5
7 - 164.1 - 163.8
8 6.39 (d, 2.1) 93.6 6.38 (br s) 93.4
9 - 156.6 - 156.3
10 - 104.0 - 103.7
1′ - 121.2 - 120.9
2′ 7.55 (m) 115.2 7.52 (br s) 115.0
3′ - 144.7 - 144.4
4′ - 148.4 - 148.1
5′ 6.85 (d, 8.9) 116.3 6.84 (d, 8.4) 116.0
6′ 7.55 (m) 121.6 7.54 (d, 8.4) 121.3
1′′ 5.35 (d,7.3) 101.2 5.32 (d, 7.2) 101.0
2′′ - 74.0 - 73.9
3′′ - 76.3 - 76.3
4′′ - 70.6 - 70.4
5′′ - 75.9 - 75.8
6′′ - 67.0 - 66.9
1′′′ 5.35 (d, 7.3) 100.7 4.37 (br s) 100.5
2′′′ - 70.4 - 70.2
3′′′ - 70.3 - 69.9
4′′′ - 71.8 - 71.7
5′′′ - 68.2 - 68.1
6′′′ 1.00 (d, 6.1) 17.7 0.98 (d, 5.1) 71.7

a Ò˜
Literature values: Ishimatsu et al., 1989 (DMSO-d 6 )

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°“√‡√◊Õß· ß¿“¬„μâ· ßÕ—≈μ√“‰«‚Õ‡≈μ ∑’˧«“¡¬“«§≈◊Ëπ Ûˆˆ ´÷Ëßμ√«®«‘‡§√“–Àå‚¥¬‡§√◊ËÕß Bruker AV 500 MHz NMR

π“‚π‡¡μ√ æ∫ “√‡ø≈‚«πÕ¬¥å ®”π«π ı-ˆ ™π‘¥ · ¥ß¥—ß Spectrometer ¢Õß»Ÿπ¬åæ—π∏ÿ«‘»«°√√¡·≈–‡∑§‚π‚≈¬’
√Ÿª∑’Ë Ò (§) ™’«¿“æ·Ààß™“μ‘ ·≈–‡ª√’¬∫‡∑’¬∫¢âÕ¡Ÿ≈§à“ chemical shift °—∫
‡¡◊ËÕπ” à«π °—¥ Nn-E3 ´÷Ë߇ªìπ “√ °—¥∑’Ë¡’ƒ∑∏‘Ï·√ß∑’Ë ÿ¥ 125 MHz ¢Õß‚ª√μÕπ·≈–§“√å∫Õπ°—∫¢âÕ¡Ÿ≈¢Õß “√∑’ˇ§¬¡’

¡“·¬°¥â«¬§Õ≈—¡π傧√¡“‚∑√°√“øï ‰¥â “√ A ·≈– “√ B √“¬ß“π¡“°àÕπ∑”„Àâ “¡“√∂æ‘ Ÿ®πå Ÿμ√‚§√ß √â“ß∑“߇§¡’¢Õß
·≈–æ‘ Ÿ®πå‡Õ°≈—°…≥å∑“߇§¡’¥â«¬‚§√¡“‚∑°√“øﺑ«∫“ß “√ A “√∫√‘ ÿ∑∏‘Ï®“°∫—«À≈«ß “√ A §◊Õ quercetin 3-O-β-D-glu-
¡’§à“ hRf ‡∑à“°—∫ ÙÙ ·≈– “√ B ¡’§à“ hRf ‡∑à“°—∫ ıÙ curonide (miquelianin) “√ B §◊Õ Rutin · ¥ß¥—ßμ“√“ß∑’Ë

(· ¥ß¥—ß√Ÿª∑’Ë Ò (§) ®“°π—Èπ π”‰ªæ‘ Ÿ®πå Ÿμ√‚§√ß √â“ß·≈– Ò ·≈– Ú
»÷°…“Õߧåª√–°Õ∫∑“߇§¡’¢Õß “√∫√‘ ÿ∑∏‘Ï∑’Ë·¬°‰¥â ‚¥¬°“√