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OR-42
Effect of ginger (Zingiber officinale Roscoe) on glucose transport in
cultured skeletal muscle cells
Kusumarn Noipha, Suvina Ratanachaiyavong
Department of Biomedical Sciences, Faculty of Medicine, Prince of Songkla University, Hat-Yai, Songkhla 90110, Thailand
Rationale: In South Asian countries, ginger (Zingiber officinale Roscoe) is widely used in traditional medicine.
Most of the previous studies have investigated in detail of its anti-diabetic effect in humans and experimental
animals. However, its hypoglycemic effect at cellular and molecular level has not been elucidated.
Objectives: The purpose of the study was to elucidate the molecular mechanistic action of the juice of Z.
officinale on glucose transport in skeletal muscle cells, L6 myotubes.
Materials and Methods: Freeze-dried fresh ginger juice was suspended in distilled water to investigate glucose
3
transport activity in L6 myotubes. Glucose transport was determined using the 2-[ H]-deoxy-D-glucose (2-DG)
uptake assay. Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) was used to analyze glucose transporter
1 and 4 (GLUT1 and GLUT4) expression. The results were expressed as mean ± SE and compared with studentûs
t test for the level of significance at p<0.05.
Results: Ginger extract (400 µg/ml) significantly increased glucose uptake in L6 myotubes after 20-h incubation
(208.03 ± 10.65% above basal value, p<0.05). This activity was partially inhibited by the protein synthesis
inhibitor, cycloheximide. The enhancement of glucose transport by ginger was accompanied by significantly
increased levels of both GLUT1 and GLUT4 mRNA expression.
Conclusion: There are several mechanisms involved in the enhancement of glucose transport by ginger in L6
myotubes. From this present study, we can conclude that the up-regulation of GLUT1 and GLUT4 expression is
an important mechanism regulating glucose transport activity by ginger.
