Page 79 - journal-6-2-S-Full

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ƒ∑∏‘ϵâ“π®ÿ≈™’æ¢Õß “√ °—¥ ¡ÿπ‰æ√µàÕ Enterococcus faecalis

Ò Ú Ú
¡“≈’ ®ß∏π“°√ , „®πÿ™ °“≠®π¿Ÿ , ÿ¿“√—µπå §”·¥ß
Ò
ΩÉ“¬∑—πµ “∏“√≥ ÿ¢ ‚√ß欓∫“≈°—π∑√≈—°…å ®—ßÀ«—¥»√’ –‡°… ÛÛÒÒ
Ú
§≥–‡¿ —™»“ µ√å ¡À“«‘∑¬“≈—¬Õÿ∫≈√“™∏“π’

«—µ∂ÿª√– ߧå Enterococcus faecalis (E. faecalis) ‡ªìπ‡™◊ÈÕ∑’ˇªì𠓇Àµÿ ”§—≠¢Õߧ«“¡≈⡇À≈«„π°“√√—°…“§≈Õß√“°øíπ
°“√«‘®—¬π’È®÷ß¡’«—µ∂ÿª√– ߧå‡æ◊ËÕ»÷°…“ƒ∑∏‘ϵâ“π‡™◊ÈÕ E. faecalis ¢Õß “√ °—¥®“° ¡ÿπ‰æ√‰∑¬ ‰¥â·°à „∫æ≈Ÿ (Piper betle
L. «ß»å Piperaceae) „∫Ω√—Ëß (Psidium guajava L. «ß»å Myrtaceae) „∫¢àÕ¬ (Streblus asper Lour. «ß»å Moraceae) ·≈–
‡ª≈◊Õ°¡—ߧÿ¥ (Garcinia mangostana L. «ß»å Guttiferae) ‚¥¬¥Ÿ®“°§à“§«“¡‡¢â¡¢âπµË” ÿ¥∑’Ë¡’ƒ∑∏‘Ϭ—∫¬—È߇™◊ÈÕ (minimum
inhibitory concentration, MIC) ·≈–§à“§«“¡‡¢â¡¢âπµË” ÿ¥∑’Ë¡’ƒ∑∏‘Ï¶à“‡™◊ÈÕ (minimum bactericidal concentration, MBC)

‡æ◊ËÕ‡ªìπ∑“߇≈◊Õ°„À¡à„π°“√æ—≤π“ ¡ÿπ‰æ√æ◊Èπ∫â“π¢Õ߉∑¬¡“∑¥·∑π°“√„™â “√‡§¡’ ·≈–‡ªìπ¢âÕ¡Ÿ≈æ◊Èπ∞“π„π°“√
æ—≤π“ ¡ÿπ‰æ√¡“„™âª√–‚¬™πå∑“ß°“√·æ∑¬å

«‘∏’¥”‡π‘π°“√ ‡µ√’¬¡ “√ °—¥À¬“∫ ¡ÿπ‰æ√„π ˘ı% ‡Õ∑“πÕ≈ ‚¥¬«‘∏’ percolation ·≈â«∑”„Àâ·Àâß‚¥¬„™â rotary evapo-
rator ™—ËßπÈ”Àπ—° “√ °—¥∑’ˉ¥â §”π«≥À“ %yield ·≈–»÷°…“ƒ∑∏‘ϵâ“π‡™◊ÈÕ E. faecalis ¢Õß “√ °—¥ ¡ÿπ‰æ√„πÀ≈Õ¥
∑¥≈Õß∑”‚¥¬„™â«‘∏’ agar disc diffusion assay ·≈– broth dilution assay ‡æ◊ËÕÀ“§à“§«“¡‡¢â¡¢âπµË” ÿ¥∑’Ë¡’ƒ∑∏‘Ϭ—∫¬—È߇™◊ÈÕ

(minimum inhibitory concentration, MIC) ·≈–§à“§«“¡‡¢â¡¢âπµË” ÿ¥∑’Ë¡’ƒ∑∏‘Ï¶à“‡™◊ÈÕ (minimum bactericidal concentra-
tion, MBC) ‚¥¬°“√∑¥≈Õßπ’ȉ¥â„™â “√¶à“‡™◊ÈÕ∑’Ë„™â„π°“√√—°…“§≈Õß√“°øíπ ‰¥â·°à ethylenediaminetetraacetic acid À√◊Õ
EDTA, chlorhexidine ·≈– sodium hypochlorite ‡ªìπ “√¡“µ√∞“π

º≈°“√»÷°…“ “√ °—¥®“°„∫æ≈Ÿ·≈–‡ª≈◊Õ°¡—ߧÿ¥¡’ƒ∑∏‘Ϭ—∫¬—Èß°“√‡®√‘≠¢Õ߇™◊ÈÕ E. faecalis „π agar disc diffusion
assay ‚¥¬¡’§à“ inhibition zone ‡∑à“°—∫ ÒÚ.ˆ±Ò.ÙÒ ·≈– ˜.Û±.ÛÚ ¡¡. µ“¡≈”¥—∫ ‡¡◊ËÕ∑¥ Õ∫¥â«¬ broth dilution

assay ‰¥â§à“§«“¡‡¢â¡¢âπµË” ÿ¥∑’Ë¡’ƒ∑∏‘Ϭ—∫¬—È߇™◊ÈÕ¢Õß “√ °—¥®“°„∫æ≈Ÿ·≈–‡ª≈◊Õ°¡—ߧÿ¥‡∑à“°—∫ ˆ.Úı ·≈– >ı ¡°./
¡≈. µ“¡≈”¥—∫ ‡©æ“– “√ °—¥®“°„∫æ≈Ÿ‡∑à“π—Èπ∑’Ë¡’ƒ∑∏‘Ï¶à“‡™◊ÈÕ E. faecalis ‚¥¬¡’§à“§«“¡‡¢â¡¢âπµË” ÿ¥∑’Ë¡’ƒ∑∏‘Ï¶à“‡™◊ÈÕ
‡∑à“°—∫ Úı ¡°./¡≈. à«π “√ °—¥®“°„∫Ω√—Ëß·≈–„∫¢àÕ¬‰¡àæ∫ƒ∑∏‘Ϭ—∫¬—Èß°“√‡®√‘≠¢Õ߇™◊ÈÕ E. faecalis „πÀ≈Õ¥∑¥≈Õß
°“√∑¥ Õ∫ƒ∑∏‘ϵâ“π‡™◊ÈÕ¢Õß “√¡“µ√∞“πæ∫«à“ “√¡“µ√∞“π∑—Èß “¡™π‘¥ ‰¥â·°à EDTA, chlorhexidine ·≈– sodium

hypochlorite ¡’ƒ∑∏‘Ï¶à“‡™◊ÈÕ E. faecalis „πÀ≈Õ¥∑¥≈Õß ‚¥¬¡’§à“§«“¡‡¢â¡¢âπµË” ÿ¥∑’Ë¡’ƒ∑∏‘Ï¶à“‡™◊ÈÕ‡∑à“°—∫ .ÙÚı,
.Úı ·≈– .ÛÒÚı ¡°./¡≈. µ“¡≈”¥—∫

¢âÕ √ÿª “√ °—¥„∫æ≈Ÿ·≈–‡ª≈◊Õ°¡—ߧÿ¥¥â«¬ ˘ı% ‡Õ∑“πÕ≈ ¡’ƒ∑∏‘Ϭ—∫¬—È߇™◊ÈÕ E. faecalis ‡©æ“– “√ °—¥„∫æ≈Ÿ¡’ƒ∑∏‘Ï
¶à“‡™◊ÈÕ E. faecalis