Page 66 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 11 ฉบับที่ 3

Journal of Thai Traditional & Alternative Medicine Vol. 11 No. 3 September-December 2013 275

√Ÿª∑’Ë 3 Ÿμ√‚§√ß √â“ß∑“߇§¡’¢Õß piscidic acid (1) ·≈– genistein 7-O-α-rhamnosyl(1 → 6)-β-glucopyranoside
(2)

endoperoxidase ·≈–‡Õπ‰´¡åμ—«π’È®–‡ª≈’ˬπμ—« ‡Õπ‰´¡å COX
‡Õ߇ªìπ thromboxane A2 ·≈– prostacyclin ∑—Èßπ’È ®“°º≈°“√·¬°Õߧåª√–°Õ∫∑“߇§¡’¢Õß “√
NSAIDs ·μà≈–μ—«· ¥ß§«“¡ “¡“√∂μà“ß°—π‰ª„π °—¥À¬“∫¥â«¬ 50% ‡Õ∑“πÕ≈¢Õ߇∂“«—≈¬å‡ª√’¬ß

°“√¬—∫¬—Èß COX ‡™àπ ¬—∫¬—Èß°“√∑”ß“π¢Õß neutro- ¥â«¬‡∑§π‘§ partition ¥â«¬ ‡Œ°‡´π, §≈Õ‚√øÕ√å¡,
phil ·≈–· ¥ßƒ∑∏‘Ï·°âª«¥®“°ª√– “∑ à«π°≈“ß ‡Õ∑‘≈Õ–´’‡μ∑, ∫‘«∑“πÕ≈ ·≈–πÈ” (√Ÿª∑’Ë 2) æ∫«à“‰¥â
à«π aspirin ÕÕ°ƒ∑∏‘χªìπ irreversible inhibitor à«π °—¥μà“ß Ê „πª√‘¡“≥∑’Ë·μ°μà“ß°—π ‚¥¬ à«π

¢Õß COX ·μà NSAIDs ∫“ßμ—« ‡™àπ ibuprofen, °—¥πÈ”¡’ª√‘¡“≥¡“°∑’Ë ÿ¥ (%yield = 44.98 ‚¥¬
piroxicam ‡ªìπ competitive reversible inhibi- πÈ”Àπ—°) ·≈– à«π °—¥‡Œ°‡´π¡’ª√‘¡“≥πâÕ¬∑’Ë ÿ¥
tor ¢Õß COX ‚¥¬ COX ·∫àßÕÕ°‡ªìπ 2 isoforms (%yield = 0.15 ‚¥¬πÈ”Àπ—°) ‡¡◊ËÕπ” à«π °—¥

§◊Õ COX-1 ·≈– COX-2 ‚¥¬ COX-1 ¡’Õ¬Ÿà„π ∑—ÈßÀ¡¥‰ª∑¥ Õ∫ƒ∑∏‘Ïμâ“πÕ—°‡ ∫‚¥¬»÷°…“ƒ∑∏‘Ï
√à“ß°“¬‡ªìπª°μ‘ à«π COX-2 ®–∂Ÿ°°√–μÿâπ¢÷Èπ„π ¬—∫¬—È߇Õπ‰´¡å COX-1 ·≈– COX-2 æ∫«à“ ‡©æ“–
™à«ß∑’Ë¡’Õ“°“√Õ—°‡ ∫ ¢âÕ¥’¢Õ߬“∑’Ë¡’ƒ∑∏‘Ϭ—∫¬—Èß COX- “√ °—¥À¬“∫·≈– à«π °—¥πÈ”‡∑à“π—Èπ∑’Ë¡’ƒ∑∏‘Ϭ—∫¬—Èß

2 §◊Õ ®–‰¡à¡’º≈„π°“√‡æ‘Ë¡°“√À≈—Ëß°√¥„π°√–‡æ“– ‡©æ“– COX-1 ‚¥¬¡’ IC50 ‡∑à“°—∫ 4.11 ·≈– 4.15
‡¥‘πÕ“À“√ „π¢≥–∑’ˬ“∑’Ë¡’ƒ∑∏‘Ϭ—∫¬—Èß COX-1 ®–¡’ ‰¡‚§√°√—¡/¡‘≈≈‘≈‘μ√ μ“¡≈”¥—∫ à«π °—¥πÈ”®÷ß∂Ÿ°
22
º≈„π°“√‡æ‘Ë¡°“√À≈—Ëß°√¥„π°√–‡æ“–Õ“À“√ ¥—ß ·¬°μàե⫬‡∑§π‘§§Õ≈—¡π傧√¡“‚∑°√“øï‚¥¬„™â
π—Èπ „π°“√»÷°…“«‘®—¬π’È ®÷ßμâÕß°“√·¬° “√∑’Ë¡’ƒ∑∏‘Ï Sephadex LH20 ‡ªìπ«—Ø¿“§§ß∑’Ë ™–¥â«¬

μâ“πÕ—°‡ ∫®“° “√ °—¥‡∂“«—≈¬å‡ª√’¬ß¥â«¬°“√§—¥ ‡¡∑“πÕ≈·≈–πÈ” √«∫√«¡ “√≈–≈“¬∑’Ë¡’≈—°…≥–
°√Õß “√∑’Ë¡’ƒ∑∏‘Ï∑“ß™’«¿“楗߰≈à“«¥â«¬°“√¬—∫¬—Èß ‡À¡◊Õπ°—π‡¡◊ËÕ«‘‡§√“–Àå¥â«¬«‘∏’‚§√¡“‚∑°√“øï