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Journal of Thai Traditional & Alternative Medicine Vol. 11 No. 3 September-December 2013 279

Abstract
Bioactive compounds of Derris scandens (Roxb.) Benth. extract
Prapai Wongsinkongman1, Jaree Bansiddhi1, Somchai Sanluangin1,
Tanavat Thongjin2, Utai Sothanapun3
1* Medicinal Plant Research Institute, Department of Medical Sciences, Nonthaburi 11000.
2 Regional Medical Sciences Center 2 Phitsanulok, Department of Medical Sciences, Phitsanulok 65000.
3 Faculty of Pharmaceutical Sciences, Silpakorn University, Nakornpathom 73000.
1*Corresponding author: prapai.w@dmsc.mail.go.th

Derris scandens (Roxb.) Benth. or thao-wan-priang has been used in Thai traditional medi-
cine as muscle pain relief, diuretic and emmenagogue. From pharmacological studies, thao-wan-
priang showed hypotensive, anti-fungal, immunostimulant, anti-inflammatory, hypoglycemia, and
free radical scavenging activities. From the preliminary study, it was found that the stem extract
of Derris scandens (Roxb.) Benth. with 50% ethanol exhibited anti-inflammatory activity against
COX-1; therefore, this study was carried out using bioassay-guided fractionation of the 50%
ethanolic extracts. As a result, two compounds were purified using column chromatography and
their chemical structures were elucidated using spectroscopic techniques, as well as comparison
with reported data. The phenolic acid and isoflavone glycoside compounds were identified as
piscidic acid (1) and genistein 7-O-α-rhamnosyl (1 → 6)-β-glucopyranoside (2), respectively. Since
compound 2 was a major chemical constituent, it was beneficial to use as a marker for the quality
control of the extract of Derris scandens (Roxb.) Benth. stems.
Key words: Derris scandens (Roxb.) Benth.; thao-wan-priang, bioactive compounds