Page 63 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 11 ฉบับที่ 3

272 «“√ “√°“√·æ∑¬å·ºπ‰∑¬·≈–°“√·æ∑¬å∑“߇≈◊Õ° ªï∑’Ë 11 ©∫—∫∑’Ë 3°—𬓬π-∏—𫓧¡ 2556

º ¡¢ÕßÕ–´’‚μ‰π‰μ√≈å: πÈ” „πÕ—μ√“ à«π 15:85 ≈–¢Õߺ≈º≈‘μ (%yield) ‡∑à“°—∫ 0.40 ·≈– 0.21
‡ªìπ«—Æ¿“§‡§≈◊ËÕπ∑’Ë Õ—μ√“°“√‰À≈∑’Ë 1.0 ¡‘≈≈‘≈‘μ√/ ‚¥¬πÈ”Àπ—° μ“¡≈”¥—∫ ‡¡◊ËÕæ‘ Ÿ®πå Ÿμ√‚§√ß √â“ß

π“∑’ ·≈–μ√«®«—¥∑’˧«“¡¬“«§≈◊Ëπ 260 π“‚π‡¡μ√ ∑“߇§¡’¢Õß “√∫√‘ ÿ∑∏‘ϥ⫬‡∑§π‘§ ‡ª°‚∑√ ‚§ªï
1
13
æ∫«à“·¬° “√∫√‘ ÿ∑∏‘Ï 1 ·≈– 2 ‰¥â∑’Ë retention time æ∫«à“¢âÕ¡Ÿ≈ H-NMR ·≈– C-NMR Õ¥§≈âÕß
5,21
‡∑à“°—∫ 1.5 ·≈– 20.5 π“∑’ μ“¡≈”¥—∫ ‚¥¬ “√ 1 °—∫º≈°“√»÷°…“„π√“¬ß“π°“√«‘®—¬∑’Ëμ’æ‘¡æå·≈â«
·≈– 2 ¡’≈—°…≥–‡ªìπºß amorphous ’¢“« ®”π«π (μ“√“ß∑’Ë 2-5) ·≈–®“° UV-VIS ·≈– MS spectra
79 ·≈– 41 ¡‘≈≈‘°√—¡ μ“¡≈”¥—∫ À√◊Õ§‘¥‡ªìπ√âÕ¬ ¢Õß “√ 1 ·≈– 2 ¡’¥—ßπ’È piscidic acid (1): λ max =

1
μ“√“ß∑’Ë 2 ¢âÕ¡Ÿ≈ H-NMR (500 MHz) ¢Õß 1 ‡ª√’¬∫‡∑’¬∫°—∫ Piscidic Acid 21
21
μ”·Àπàß‚ª√μÕπ δ (ppm) „π d -DMSO ¢Õß 1 δ (ppm) „π CD OD ¢Õß piscidic acid
H 6 H 3
1 9.12 (2H, br s) -
2 4.24 (1H, s) 4.55 (1H, s)
4 2.80 (1H, d, 13.74), 2.97 (2H, m)
2.94 (1H, d, 13.73)
5 9.12 (2H, br s) -
2' 6.97 (2H, d, 8.54) 7.04 (2H, d, 7.9)
3' 6.60 (2H, d, 8.54) 6.72 (2H, d, 7.9)
4' 3.45 (1H, br s) -
5' 6.60 (2H, d, 8.54) 6.72 (2H, d, 7.9)
6' 6.97 (2H, d, 8.54) 7.04 (2H, d, 7.9)

13
μ“√“ß∑’Ë 3 ¢âÕ¡Ÿ≈ C-NMR (125 MHz) ¢Õß 1 ‡ª√’¬∫‡∑’¬∫°—∫ Piscidic Acid 21
21
μ”·Àπàߧ“√å∫Õπ δ (ppm) „π d -DMSO ¢Õß 1 δ (ppm) „π CD3OD ¢Õß piscidic acid
C
C
6
1 172.89 174.88
2 75.25 76.57
3 79.59 81.44
4 40.62 41.98
5 174.05 176.18
1' 121.52 128.10
2' 131.15 132.16
3' 114.52 116.24
4' 155.81 156.53
5' 114.52 116.24
6' 131.15 132.16