Page 60 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 11 ฉบับที่ 3

Journal of Thai Traditional & Alternative Medicine Vol. 11 No. 3 September-December 2013 269

Laboritechnik) ‡§√◊ËÕß Rotavapor √ÿàπ R-114 (Barnstead)
(Buchi) ‡§√◊ËÕß Aspirator √ÿàπ A-3S ¬’ËÀâÕ Eyela ®
«‘∏’°“√»÷°…“
·≈–‡§√◊ËÕß∑”πÈ”‡¬ÁπÀ¡ÿπ‡«’¬π √ÿàπ CA-101 ¬’ËÀâÕ
Eyela (Tokyo Rikakikai) 1. °“√‡μ√’¬¡ “√ °—¥À¬“∫¥â«¬ 50% ‡Õ∑“π
5. ‡§√◊ËÕß√–‡À‘¥„Àâ·Àâߥ⫬§«“¡‡¬Áπ (Freeze- Õ≈¢Õß≈”μâπ‡∂“«—≈¬å‡ª√’¬ß

dried Apparatus) (Labconco) ≈”μâπ (‡∂“) ‡∂“«—≈¬å‡ª√’¬ß (0.8 °‘‚≈°√—¡) ‡°Á∫
6. ‡§√◊ËÕß™—Ë߉øøÑ“ æ‘°—¥ 0.1-0.01 ¡‘≈≈‘°√—¡ ®“°Õ”‡¿Õ∫“ß°√–∑ÿà¡ ®—ßÀ«—¥æ‘…≥ÿ‚≈° „πªï æ.».
(Sartorius) 2548 ‡¡◊ËÕμ√«® Õ∫™◊ËÕ«‘∑¬“»“ μ√åμ“¡À≈—°æƒ°…

7. Õà“߇ ’¬ß§«“¡∂’Ë Ÿß (Ultrasonic bath) Õπÿ°√¡«‘∏“π ‚¥¬„™â‡Õ° “√ª√–°Õ∫¥â“π惰…™“μ‘
1,2
(Elma) ¢Õßμà“ߪ√–‡∑» æ∫«à“§◊Õ Derris scandens
8. ‡§√◊ËÕß·¬°‚§√¡“‚∑°√“ø ¡√√∂π– Ÿß Benth. «ß»å Leguminosae π”μ—«Õ¬à“ß ¥¡“≈â“ß

(Analytical HPLC) (Waters) ª√–°Õ∫¥â«¬ πÈ”„Àâ –Õ“¥ º÷Ëß„Àâ·Àâß∑’ËÕÿ≥À¿Ÿ¡‘ÀâÕßæÕÀ¡“¥Ê
‡§√◊ËÕߧ«∫§ÿ¡ªíö¡ √ÿàπ 600 ‡§√◊ËÕß©’¥ “√μ—«Õ¬à“ß√ÿàπ À—Ëπ‡ªìπ™‘Èπ·≈–Õ∫„Àâ·Àâߥ⫬μŸâÕ∫√âÕπ‰øøÑ“∑’ËÕÿ≥À¿Ÿ¡‘
717 §Õ≈—¡π嬒ËÀâÕ X-Terra RP18 ¢π“¥ 4.6 ± 150 45-50 Õß»“‡´≈‡´’¬ π“π 48 ™—Ë«‚¡ß ·≈â«π”

‡´π쑇¡μ√, 60 Å, 5 ‰¡‚§√‡¡μ√ ·≈–‡§√◊ËÕßμ√«® μ—«Õ¬à“ß∑’Ë·Àâß·≈⫉ª∫¥‡ªìπºßÀ¬“∫ °—¥¥â«¬ 50%
«—¥™π‘¥‚ø‚쉥‚Õ¥·Õ‡√¬å√ÿàπ 2996 ‡Õ∑“πÕ≈¥â«¬«‘∏’ Soxhlet π“π 2 ™—Ë«‚¡ß ∑”´È” 2
9. ‡§√◊ËÕß·¬°‚§√¡“‚∑°√“ø ¡√√∂π– Ÿß™π‘¥ §√—Èß √«¡ “√ °—¥∑’ˉ¥â·≈â«√–‡À¬„À⇢⡢âπ¥â«¬
preparative (Preparative HPLC) (Waters) ‡§√◊ËÕß√–‡À¬ ÿ≠≠“°“» ·≈–∑”„Àâ·Àâߥ⫬«‘∏’°“√

ª√–°Õ∫¥â«¬‡§√◊ËÕߧ«∫§ÿ¡ªíö¡ √ÿàπ 600 §Õ≈—¡πå Prep ·™à·¢Áß·≈â«√–‡À‘¥„Àâ·Àâß (freeze-dried method)
R
Novapak HRC18 (25×100 ¡¡, 6 ‰¡‚§√‡¡μ√) ‰¥â “√ °—¥À¬“∫ 142.81 ° (%yield = 17.85 ‚¥¬
μàÕ°—∫ RCM guard insert HRC18 (25×10 πÈ”Àπ—°)
¡‘≈≈‘‡¡μ√, 6 ‰¡‚§√‡¡μ√) ·≈–‡§√◊ËÕßμ√«®«—¥™π‘¥ 2. °“√·¬°Õߧåª√–°Õ∫∑“߇§¡’®“° “√ °—¥
photodiode array detector (PDA) √ÿàπ 2996 À¬“∫¥â«¬ 50% ‡Õ∑“πÕ≈¢Õ߇∂“«—≈¬å‡ª√’¬ß
10. “√‡§¡’∑ÿ°™π‘¥∑’Ë„™â„π°“√∑¥≈Õßμà“ßÊ “√ °—¥À¬“∫¥â«¬ 50% ‡Õ∑“πÕ≈¢Õ߇∂“«—≈¬å

‡ªìπ™π‘¥∑’Ë„™â°—∫ß“π«‘‡§√“–Àå (analytical grade) ‡ª√’¬ß∑’Ë·Àâß·≈â« ®”π«π 130 °√—¡ ∂Ÿ° partition ¥â«¬
·≈–πÈ”∫√‘ ÿ∑∏‘Ï∑’Ë„™â„πß“π∑¥≈Õ߇ªìππÈ”∑’ˉ¥â®“°°“√ μ—«∑”≈–≈“¬™π‘¥μà“ß Ê ‰¥â·°à ‡Œ°‡´π, §≈Õ‚√øÕ√å¡,

°√Õ߇ՓÕπÿ¿“§∑’Ë¡’ª√–®ÿÕÕ° (deionized water) ‡Õ∑‘≈Õ–´’‡μ∑, ∫‘«∑“πÕ≈ ·≈–πÈ” ‚¥¬§à“√âÕ¬≈–
11. “√‡§¡’∑ÿ°™π‘¥∑’Ë„™â°—∫‡§√◊ËÕß‚§√¡“‚∑ ¢Õߪ√‘¡“≥ “√ °—¥·≈–ª√‘¡“≥º≈º≈‘μ · ¥ß„π
°√“ø ¡√√∂π– Ÿß (HPLC) ‡ªìπ HPLC, gradient √Ÿª∑’Ë 2 ·≈–ƒ∑∏‘Ï¢Õß à«π °—¥μà“ßÊ · ¥ß„πμ“√“ß∑’Ë
grade (Merck) ·≈–πÈ”∫√‘ ÿ∑∏‘ÏμâÕß°√Õ߇Փ 1

Õπÿ¿“§∑’Ë¡’ª√–®ÿÕÕ° ‚¥¬„™â‡§√◊ËÕß°√ÕßπÈ”∫√‘ ÿ∑∏‘Ï ®“°º≈°“√∑¥ Õ∫ƒ∑∏‘Ï„πÀ≈Õ¥∑¥≈ÕßμàÕ
(Ultra Pure Water System) ¬’ËÀâÕ Nanopure °“√¬—∫¬—Èß COX-1 ·≈– COX-2 æ∫«à“ “√ °—¥