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Journal of Thai Traditional & Alternative Medicine
Journal of Thai Traditional & Alternative Medicine
Journal of Thai Traditional & Alternative Medicine Vol. 9 No. 1 January-August (Supplement) 2011 51
Journal of Thai Traditional & Alternative Medicine
Journal of Thai Traditional & Alternative Medicine
PC-10

§«“¡‡ªìπæ‘…μàÕ‡´≈≈å„πÀ≈Õ¥∑¥≈ÕߢÕß Labdane-type diterpenes ®“°‡Àßâ“

·≈–¥Õ°¡À“Àß å (Hedychium coronarium)

1 2 2 1,2
°“πμ噑…™π° “√ ÿ¢ , π‘μ‘√—μπå ©‘¡πâÕ¬ , π‘»“™≈ §ÿ≥«ÿ≤‘¡‚π∏√√¡ , ÿæ√√≥“ ‡μ™– °ÿ≈
1
§≥–«‘∑¬“»“ μ√å ¡À“«‘∑¬“≈—¬‡°…μ√»“ μ√å
2
∂“∫—π«‘®—¬®ÿÓ¿√≥å

À≈—°°“√·≈–‡Àμÿº≈ ¡À“Àß å‡ªìπæ◊™«ß»å‡¥’¬«°—∫¢‘ß (Zingiberaceae) ‡Àßâ“ “¡“√∂·°â°…—¬ ‰μæ‘°“√ ∫”√ÿß°”≈—ß ·°âø°™È”
·≈–‡ªìπ¬“Õ“¬ÿ«—≤π– ¥Õ°√—°…“Õ“°“√ª«¥À—« ®“°°“√μ√«® Õ∫ß“π«‘®—¬∑’˺à“π¡“æ∫«à“ “√ª√–°Õ∫ à«π„À≠à‡ªìπ “√®”æ«°

Labdane-type diterpenes ∫“ß™π‘¥ “¡“√∂¬—∫¬—È߇´≈≈å¡–‡√Áß∫“ß™π‘¥‰¥â ·μà°“√∑¥ Õ∫§«“¡‡ªìπæ‘…μàÕ‡´≈≈å¡–‡√Áß™π‘¥μà“ß Ê
¬—߉¡à§√Õ∫§≈ÿ¡ ß“π«‘®—¬π’È®÷ß¡ÿà߇πâπ°“√ μ√«® Õ∫À“ “√ª√–°Õ∫∑’Ëæ∫„π‡Àßâ“·≈–¥Õ°¡À“Àß å√«¡∂÷ߧ«“¡‡ªìπæ‘…μàÕ‡´≈≈å
¡–‡√Áß™π‘¥μà“ß Ê
«—μ∂ÿª√– ß§å »÷°…“°“√·¬° “√ª√–°Õ∫ °“√μ√«® Õ∫‚§√ß √â“ß·≈–§«“¡‡ªìπæ‘…μàÕ‡´≈≈å¡–‡√ÁߢÕß “√ª√–°Õ∫∑’ˉ¥â®“°
‡Àßâ“μ“°·Àâß·≈–¥Õ° ¥¢Õß¡À“Àß å ‡æ◊ËÕ𔉪 Ÿà°“√æ—≤𓇪ì𬓄πÕπ“§μ

«‘∏’¥”‡π‘π°“√ π”‡Àßâ“μ“°·Àâß·≈–¥Õ° ¥¡À“Àß å (À“°„™â¥Õ°μ“°·Àâßæ∫«à“ “√∫“ß™π‘¥∂Ÿ°∑”≈“¬) ¡“ºà“π°“√ °—¥ ‚¥¬
‡Àßâ“ °—¥¥â«¬ dichloromethane à«π¥Õ° °—¥¥â«¬ dichloromethane ®“°π—Èπ °—¥μàե⫬ methanol ®–‰¥â “√ °—¥À¬“∫
®“°π—Èππ” “√ °—¥À¬“∫·μà≈– à«π¡“·¬°‚¥¬„™â°√–∫«π°“√∑“ß chromatography method ®π‰¥â “√∫√‘ ÿ∑∏‘Ï μ√«® Õ∫

≈—°…≥–‚§√ß √â“ߢÕß “√∫√‘ ÿ∑∏‘Ï‚¥¬„™â«‘∏’ spectroscopy method ·≈–μ√«® Õ∫§«“¡‡ªìπæ‘…μàÕ‡´≈≈å¡–‡√ÁߢÕß “√ª√–°Õ∫
‚¥¬„™â «‘∏’ MTT assay
º≈°“√»÷°…“ °“√·¬° “√ª√–°Õ∫®“°‡Àßâ“ æ∫ “√„À¡à 1 ™π‘¥ [7-α-hydroxy-(E)-labda-8(17), 12-diene-15, 16-dial]
·≈– “√ª√–°Õ∫°≈ÿà¡ labdane-type diterpenes 6 ™π‘¥ [(E)-labda-8(17),12-diene-15,16-dial, coronarin D, coronarin D
methyl ether, coronarin D ethyl ether, isocoronarin D ·≈– coronarin B] æ∫ “√ª√–°Õ∫®“°¥Õ°¡À“Àß å

(dichloromethane extract) 3 ™π‘¥ [(E)-labda-8(17), 12-diene-15, 16-dial, coronarin D ·≈– coronarin B] æ∫ “√ª√–°Õ∫
®“°¥Õ°¡À“Àß å (methanol extract) 5 ™π‘¥ [ “√º ¡ methyl jasmonate, “√º ¡ jasmonic acid ·≈– oxindol] ®“°
¢âÕ¡Ÿ≈ 1H ·≈– 13C-NMR æ∫«à“ coronarin D, coronarin D methyl ether ·≈– coronarin D ethyl ether ∑’Ëμ”·Àπàß C-15

‡ªìπ epimers °“√∑”Õπÿæ—π∏å¢Õß coronarin D “¡“√∂¬◊π¬—𧫓¡‡ªìπ epimers ∑’Ëμ”·Àπàß C-15 ¢Õß coronarin D
πÕ°®“°π’Ȭ—ßæ∫«à“ coronarin D, isocoronarin D, coronarin B ·≈– coronarin D acetate ¡’§«“¡‡ªìπæ‘…μàÕ‡´≈≈å¡–‡√Áß¡’§à“
IC μË”°«à“ 4 μgml-1
50
¢âÕ √ÿª æ∫ “√ª√–°Õ∫∑—ÈßÀ¡¥ 12 ™π‘¥ à«π„À≠à‡ªìπ “√ª√–‡¿∑ labdane-type diterpenes ‚¥¬æ∫ “√„À¡à 1 ™π‘¥
§◊Õ 7-α-hydroxy-(E)-labda-8(17), 12-diene-15,16-dial πÕ°®“°π’Èæ∫«à“‡ªìπ§√—Èß·√°∑’Ë¡’°“√§âπæ∫ “√º ¡ jasmonic acid

·≈– oxindol „π¥Õ° ¥¡À“Àß å “√ª√–°Õ∫∫“ß™π‘¥∑’Ë·¬°‰¥â¡’§«“¡‡ªìπæ‘…μàÕ‡´≈≈å¡–‡√Áß