Page 61 - journal-8-23-Full

50 «“√ “√°“√·æ∑¬å·ºπ‰∑¬·≈–°“√·æ∑¬å∑“߇≈◊Õ°«“√ “√°“√·æ∑¬å·ºπ‰∑¬·≈–°“√·æ∑¬å∑“߇≈◊Õ° ªï∑’Ë 9©∫—∫∑’Ë 1 ¡°√“§¡- ‘ßÀ“§¡ (©∫—∫‡ √‘¡) 2554
«“√ “√°“√·æ∑¬å·ºπ‰∑¬·≈–°“√·æ∑¬å∑“߇≈◊Õ°
«“√ “√°“√·æ∑¬å·ºπ‰∑¬·≈–°“√·æ∑¬å∑“߇≈◊Õ°å·ºπ‰∑¬·≈–°“√·æ∑¬å∑“߇≈◊Õ°
«“√ “√°“√·æ∑¬
PC-9

º≈¢Õß “√ °—¥‡≈◊Õ¥§«“¬„∫‡≈Á° Gymnacranthera eugeniifolia (A. DC.) J. Sincl.

μàÕ‡™◊ÈÕ Staphylococcus aureus ATCC 25923

1 2 2
ªî¬π—π∑å ¬–¿—°¥’ , ´ÿŒ—¬≈“ –¡–·Õ , –Õÿ¥’ ¡–ª√– ‘∑∏‘Ï
1
π—°»÷°…“À≈—° Ÿμ√«‘∑¬“»“ μ√∫—≥±‘μ ( “∏“√≥ ÿ¢™ÿ¡™π) «‘∑¬“≈—¬°“√ “∏“√≥ ÿ¢ ‘√‘π∏√ ®—ßÀ«—¥¬–≈“
2
Õ“®“√¬å°≈ÿà¡ß“πÀ≈—° Ÿμ√«‘∑¬“»“ μ√∫—≥±‘μ «‘∑¬“≈—¬°“√ “∏“√≥ ÿ¢ ‘√‘π∏√ ®—ßÀ«—¥¬–≈“

À≈—°°“√·≈–‡Àμÿº≈ ª√–‡∑»‰∑¬‡√’¬π√Ÿâ°“√„™â ¡ÿπ‰æ√„π°“√√—°…“‚√§„π™ÿ¡™π¡“π“πÀ≈“¬æ—πªï®π‡°‘¥‡ªìπ«—≤π∏√√¡
·≈–¿Ÿ¡‘ªí≠≠“ ¥—ߪ√–™“™π„πÕ”‡¿Õ‡¡◊Õß ®—ßÀ«—¥¬–≈“ ‰¥â¡’°“√„™â à«πμà“ß Ê ¢Õßμâπ‡≈◊Õ¥§«“¬„∫‡≈Á°¡“‡ªìπ¬“√—°…“‚√§º‘«Àπ—ß

ºŸâ»÷°…“®÷ß π„®∑’Ë®–»÷°…“¿Ÿ¡‘ªí≠≠“∑âÕß∂‘Ëπ„π°“√√—°…“‚√§º‘«Àπ—ß·≈–»÷°…“ª√– ‘∑∏‘º≈∑“ß«‘∑¬“»“ μ√å∑’Ë¡’º≈„π°“√¬—∫¬—È߇™◊ÈÕ
·∫§∑’‡√’¬∑’Ë∑”„À⇰‘¥‚√§º‘«Àπ—ß ‡æ◊ËÕπ”º≈°“√»÷°…“¡“„™âª√–‚¬™πå„π°“√¥Ÿ·≈√—°…“ ÿ¢¿“æ·∫∫æ÷Ëßæ“μπ‡Õßμ“¡·π«π‚¬∫“¬
¢Õß°√–∑√«ß “∏“√≥ ÿ¢ ´÷Ëß®–‡ªìπª√–‚¬™πåÕ¬à“߬‘Ëß ”À√—∫ª√–™“™π§π‰∑¬
«—μ∂ÿª√– ß§å ‡æ◊ËÕ»÷°…“º≈¢Õß “√ °—¥®“°æ◊™ ¡ÿπ‰æ√μàÕ‡™◊ÈÕ Staphylococcus aureus ATCC 25923

«‘∏’¥”‡π‘π°“√ »÷°…“¿Ÿ¡‘ªí≠≠“∑âÕß∂‘Ëπ‚¥¬°“√ —¡¿“…≥å ·≈–»÷°…“º≈¢Õß “√ °—¥‡≈◊Õ¥§«“¬„∫‡≈Á° „πÀâÕߪؑ∫—μ‘°“√ ‚¥¬
π” „∫ °‘Ëß ·≈–º≈¢Õ߇≈◊Õ¥§«“¬„∫‡≈Á°·™à¥â«¬ ‡Õ∑“πÕ≈ 95 ‡ªÕ√凴Áπμå°√Õ߇Փ “√ °—¥ √–‡À¬μ—«∑”≈–≈“¬ÕÕ°¥â«¬ Rotary
evaporator ·≈–‰¥â “√ °—¥À¬“∫ ®“°π—Èππ” “√ °—¥¥—ß°≈à“«∑¥ Õ∫°“√¬—∫¬—È߇™◊ÈÕ‡∫◊ÈÕßμâπ μàÕ‡™◊ÈÕ S. aureus ATCC 25923
¥â«¬«‘∏’ disc diffusion method ·≈–∑¥ Õ∫À“§à“ Minimal Inhibitory Concentration (MIC), Minimal bactericidal

concentration (MBC) ‚¥¬«‘∏’ broth macrodilution method
º≈°“√»÷°…“ º≈°“√∑¥ Õ∫‡¡◊ËÕπ”‡Õ“·ºàπ disc ∑’Ë¡’ “√ °—¥À¬“∫®“°„∫ °‘Ëß ·≈–º≈¢Õ߇≈◊Õ¥§«“¬„∫‡≈Á°∑’Ë °—¥¥â«¬
‡Õ∑“πÕ≈ 95 ‡ªÕ√凴Áπμå π”¡“∑¥ Õ∫ƒ∑∏‘Ïμâ“π‡™◊ÈÕ S. aureus ATCC 25923 ‡∫◊ÈÕßμâπ¥â«¬«‘∏’ disc diffusion method æ∫«à“
“√ °—¥∑—Èß “¡· ¥ß°“√ÕÕ°ƒ∑∏‘Ϭ—∫¬—Èß°“√‡®√‘≠¢Õ߇™◊ÈÕ S. aureus ATCC 25923 ‚¥¬„Àâ§à“ Inhibition zone Õ¬Ÿà„π™à«ß 9 - 14

¡‘≈≈‘‡¡μ√ ‡©≈’ˬ 11.94 ± 2.37 ¡‘≈≈‘‡¡μ√ ·≈–∑¥ Õ∫À“§à“ Minimal Inhibitory Concentration (MIC) ¢Õß “√ °—¥„∫ °‘Ëß
·≈–º≈¢Õ߇≈◊Õ¥§«“¬„∫‡≈Á°μàÕ‡™◊ÈÕ S. aureus ATCC 25923 æ∫«à“ “√ °—¥„∫ °‘Ëß ·≈–º≈ „Àâ§à“ MIC Õ¬Ÿà„π™à«ß 128 - 512
‰¡‚§√°√—¡ / ¡‘≈≈‘≈‘μ√ ·≈–§à“§«“¡‡¢â¡¢âπμË” ÿ¥∑’Ë “¡“√∂¶à“‡™◊ÈÕ‰¥â Minimal bactericidal concentration (MBC) æ∫«à“§à“
MBC ¢Õß “√ °—¥„∫ °‘Ëß ·≈–º≈ Õ¬Ÿà„π™à«ß 256 - 1,024 ‰¡‚§√°√—¡ / ¡‘≈≈‘≈‘μ√ à«π¢Õß¿Ÿ¡‘ªí≠≠“∑âÕß∂‘Ëππ”‡Õ“‡¡≈Á¥¢Õß

‡≈◊Õ¥§«“¬„∫‡≈Á°∑’Ë·Àâß¡“‡§’ˬ«º ¡°—∫πÈ”¡—πæ◊™¥â«¬‰øª“π°≈“ß„™â‡«≈“„π°“√‡§’ˬ«ª√–¡“≥ 2 ™—Ë«‚¡ß ∑‘È߉«â„À⇬Áπ ·≈–𔉪„™â
„π°“√√—°…“‚√§º‘«Àπ—ß
¢âÕ √ÿª “√ °—¥¥â«¬‡Õ∑“πÕ≈®“°„∫¢Õ߇≈◊Õ¥§«“¬„∫‡≈Á°¡’º≈¬—∫¬—È߇™◊ÈÕ S. aureus ATCC 25923 ·≈– “¡“√∂æ—≤π“
‡ªìπº≈‘μ¿—≥±å‡æ◊ËÕ„™â„π°“√√—°…“‚√§º‘«Àπ—ß∑’ˇ°‘¥®“°‡™◊ÈÕ S. aureus ATCC 25923 ‰¥â