Page 133 - journal-6-3-Full

ÛÙÚ «“√ “√°“√·æ∑¬å·ºπ‰∑¬·≈–°“√·æ∑¬å∑“߇≈◊Õ° ªï∑’Ë ˆ©∫—∫∑’Ë Û°—𬓬π-∏—𫓧¡ ÚııÒ

ªí®®—¬‡ ’Ë¬ß ”§—≠µàÕ°“√‡°‘¥¿“«–·∑√°´âÕπ√ÿπ·√ß„πºŸâªÉ«¬
¿Ÿ¡‘À≈—ß·≈–‡Àµÿº≈
‡∫“À«“π.
§πª√°µ‘ “¡“√∂§«∫§ÿ¡√–¥—∫πÈ”µ“≈„π‡≈◊Õ¥À≈—ßÕ“À“√

(Postprandial glucose: PPG) ‚¥¬Õ“»—¬ŒÕ√å‚¡πÕ‘π´Ÿ≈‘π √–‡∫’¬∫«‘∏’»÷°…“
·µà„πºŸâªÉ«¬‡∫“À«“π‡¡◊ËÕ∫√‘‚¿§Õ“À“√‰¡à “¡“√∂À≈—ËßÕ‘π´Ÿ≈‘π
“√‡§¡’·≈–Õÿª°√≥å
‰¥â‡æ’¬ßæÕ∑’Ë®–§«∫§ÿ¡√–¥—∫πÈ”µ“≈„π‡≈◊Õ¥∑’ˇæ‘Ë¡¢÷Èπ. °“√

‡æ‘Ë¡¢÷Èπ¢Õß√–¥—∫πÈ”µ“≈À≈—ßÕ“À“√„Àâº≈√⓬µàÕ√–∫∫°“√‰À≈ “√‡§¡’∑’Ë„™â ‰¥â·°à alloxan monohydrate (Sigma
‡«’¬π‡≈◊Õ¥¡“°°«à“°“√‡æ‘Ë¡¢÷Èπ¢Õß√–¥—∫πÈ”µ“≈„π‡≈◊Õ¥À≈—ß Chemicals), chlorpropamide (Sigma Chemicals),

Õ¥Õ“À“√ (fasting plasma glucose: FPG). „πºŸâªÉ«¬‡∫“ acabose (Glucobay , Bayer), °≈Ÿ‚§ (D-glucose, Merck),

À«“π°“√‡°‘¥¿“«–‡≈◊Õ¥¡’πÈ”µ“≈¡“°‡°‘πÀ≈—ßÕ“À“√ (postpran- ´Ÿ‚§√ (Merck), perchloric acid (Merck), Peridochrom
dial hyperglycemia) ‡ªìπªí®®—¬‡ ’ˬß∑’Ë ”§—≠µàÕ°“√‡°‘¥ Glucose (Boehringer Mannheim), πÈ”‡°≈◊Õ .˘%
‚√§À—«„®·≈–À≈Õ¥‡≈◊Õ¥ ·≈–‚√§·∑√°´âÕπ∑’Ë√ÿπ·√ßÕ◊Ëπ Ê. (Õߧ尓√‡¿ —™°√√¡), copper(II) sulfate pentahydrate

πÕ°®“°π—Èπ ºŸâ∑’Ë¡’√–¥—∫ FPG ª√°µ‘ ·µà Ÿ≠‡ ’¬§«“¡∑π°≈Ÿ‚§ (Riedel de Hagen AG), potassium sodium tartrate
(impaired glucose tolerance test) ®–¡’§«“¡‡ ’ˬߵàÕ°“√ (Merck), sodium hydroxide (BDH), ·ºàπ·¡°π’‡´’¬¡ (Mg

‡°‘¥‚√§À—«„®·≈–À≈Õ¥‡≈◊Õ¥‡æ‘Ë¡¢÷Èπ ·≈–æ—≤𓉪‡ªìπ‚√§ Ribbon) ·≈–°√¥‡°≈◊Õ‡¢â¡¢âπ (Merck).
Ò-Û
‡∫“À«“π‰¥â . ¥—ßπ—Èπ°“√§«∫§ÿ¡√–¥—∫πÈ”µ“≈„π‡≈◊Õ¥À≈—ß ‡§√◊ËÕß¡◊Õ∑’Ë„™â ‰¥â·°à ‡§√◊ËÕß√–‡À¬·Àâß·∫∫ ÿ≠≠“°“»
Õ“À“√„ÀâÕ¬Ÿà„π‡°≥±åª√°µ‘®÷ß¡’§«“¡ ”§—≠∑—Èß„πºŸâªÉ«¬ (Eyela, Tokyo Rikakikai), ‡§√◊ËÕß∑”„Àâ·Àâß‚¥¬°“√√–‡À‘¥

‡∫“À«“π·≈–ºŸâ∑’Ë Ÿ≠‡ ’¬§«“¡∑π°≈Ÿ‚§ . ¥â«¬§«“¡‡¬Áπ (Labconco), ‡§√◊ËÕß‚§√¡“‚µ°√“øï ¡√√∂π–
À¡àÕπ (Morus alba L. «ß»å Moraceae) ‡ªìπæ◊™∑’Ë Ÿß (HPLC) (Waters), §Õ≈—¡πå HPLC ™π‘¥ C18 (X-Terra,
Ù
æ∫‰¥â∑—Ë«‰ª„πªÉ“¥‘∫ . πÕ°®“°®–„™â„∫À¡àÕπ„π°“√‡≈’Ȭ߉À¡ Waters), ‡§√◊ËÕß«‘‡§√“–Àå™’«‡§¡’‡≈◊Õ¥Õ—µ‚π¡—µ‘ (Hitashi Au-
ı ˆ
·≈â« ·æ∑¬åæ◊Èπ∫â“π„πÀ≈“¬ª√–‡∑» ‡™à𠇪π , ‡ª√Ÿ , tomatic Analyzer 912) ·≈–‡§√◊ËÕߪíòπ‡À«’ˬ߷∫∫‰¡‚§√
˜ ¯
¬Ÿ‚° ≈“‡«’¬ ·≈–™‘≈’ „™â„∫À¡àÕπ ”À√—∫√—°…“ºŸâªÉ«¬‡∫“À«“π. ™π‘¥§«∫§ÿ¡Õÿ≥À¿Ÿ¡‘ (Jouan BR4i Multifunction Centri-
¢âÕ¡Ÿ≈°“√»÷°…“«‘®—¬„π —µ«å∑¥≈Õ߬—ßæ∫«à“ “√ °—¥„∫ fuge).
À¡àÕπ¡’ƒ∑∏‘Ï„π°“√≈¥√–¥—∫πÈ”µ“≈„π‡≈◊Õ¥∑—Èß„πÀπŸ·√µ
˘-Òˆ ¡ÿπ‰æ√
ª√°µ‘·≈–ÀπŸ·√µ∑’Ë∂Ÿ°‡Àπ’ˬ«π”„À⇪ìπ‡∫“À«“π . Oku T
Ò˜
·≈–§≥– √“¬ß“π«à“ “√ °—¥®“°„∫À¡àÕπ¡’ƒ∑∏‘Ϭ—∫¬—Èß „∫À¡àÕπÕ∫·Àâß ´◊ÈÕ®“°√â“π§â“ àß „π°√ÿ߇∑æœ (™“‡√πÕß)
‡Õπ‰´¡å disaccharidase „π≈”‰ â‡≈Á°¢Õߧπ·≈–ÀπŸ·√µ ‡ª√’¬∫‡∑’¬∫¢âÕ¡Ÿ≈‡Õ°≈—°…≥å∑“߇§¡’¥â«¬«‘∏’ TLC ·≈– HPLC
¥—ßπ—Èπ®÷߇™◊ËÕ‰¥â«à“ “√ °—¥„∫À¡àÕπ®–¡’ƒ∑∏‘Ϭ—∫¬—Èß°“√‡æ‘Ë¡¢÷Èπ ¢Õßµ—«Õ¬à“ß (À¡“¬‡≈¢«‘‡§√“–Àå MaR0051) °—∫µ—«Õ¬à“ß„∫

¢Õß√–¥—∫πÈ”µ“≈„π‡≈◊Õ¥À≈—ßÕ“À“√‰¥â. §≥–ºŸâ«‘®—¬π’È®÷ß À¡àÕπ¢Õß·∑⮓°»Ÿπ¬åµ√«® Õ∫·≈–√—∫√Õߧÿ≥¿“æ ¡ÿπ‰æ√
∑”°“√»÷°…“ƒ∑∏‘Ï¢Õß “√ °—¥„∫À¡àÕπµàÕ¿“«–‡≈◊Õ¥¡’πÈ”µ“≈ °√¡«‘∑¬“»“ µ√å°“√·æ∑¬å (À¡“¬‡≈¢«‘‡§√“–Àå ˆ/Ù˜).

¡“°‡°‘πÀ≈—ßÕ“À“√„πÀπŸ·√µª√°µ‘·≈–ÀπŸ·√µ‡∫“À«“π ·≈–
°“√ °—¥·≈–°“√‡µ√’¬¡ “√ °—¥·¬° à«π
À“°≈ÿà¡ “√∑’ˇªìπµ—«ÕÕ°ƒ∑∏‘Ï ‡æ◊ËÕ„™â„π°“√§«∫§ÿ¡§ÿ≥¿“æ
“√ °—¥ ‚¥¬À«—ß«à“¢âÕ¡Ÿ≈∑’ˉ¥â®–‡ªìπª√–‚¬™πåµàÕ°“√æ—≤π“ π”„∫À¡àÕπ·Àâß¡“∫¥„Àâ≈–‡Õ’¬¥ ·≈⫵⡠°—¥¥â«¬

„∫À¡àÕπ ·≈–°“√π” “√ °—¥„∫À¡àÕπ‰ª‡ªìπº≈‘µ¿—≥±å„™â„π ‡Õ∑“πÕ≈ ˘ı%, ‡Õ∑“πÕ≈ ı% ·≈–πÈ”. π” à«π °—¥∑’ˉ¥â
°“√ªÑÕß°—π°“√‡°‘¥‡∫“À«“π„πª√–™“°√°≈ÿࡇ ’Ë¬ß ‡™àπ ¡’ ¡“√–‡À¬·Àâߥ⫬‡§√◊ËÕß√–‡À¬·Àâß·∫∫ ÿ≠≠“°“» À√◊Õ∑”„Àâ

¿“«–Õâ«π À√◊Õ¿“«–‰¡à∑π°≈Ÿ‚§ (glucose intolerance) ·Àâß‚¥¬°“√√–‡À‘¥¥â«¬§«“¡‡¬Áπ (√Ÿª∑’Ë Ò).
À√◊Õ„™â§«∫§ÿ¡¿“«–‡≈◊Õ¥¡’πÈ”µ“≈¡“°‡°‘πÀ≈—ßÕ“À“√ ´÷Ë߇ªìπ π” “√ °—¥‡Õ∑“πÕ≈ ˘ı% ‰ª °—¥·¬° à«πµàե⫬