Page 76 - journal-6-2-S-Full

Journal of Thai Traditional & Alternative Medicine Vol. 6 No. 2 May-August (Supplement) 2008 ˜ı

OR-35

“√ °—¥·≈– “√ÕÕ°ƒ∑∏‘Ï®“°‰æ≈¬—∫¬—Èß°“√ ≈“¬°√–¥Ÿ°ÕàÕπ„πÀ≈Õ¥∑¥≈Õß

Ò Ò Ò Ú
»‘√‘«√√≥ Õߧ剙¬ , √ÿ®‘‡√¢ ‰™¬«ß…“ , ª√—™≠“ §ß∑«’‡≈‘» , «‘™—¬ √‘È«µ√–°Ÿ≈
Ò
Àπ૬«‘®—¬∑’Ë¡’§«“¡‡ªìπ‡≈‘»∑“ߥâ“π«‘»«°√√¡‡π◊ÈÕ‡¬◊ËÕ ¿“§«‘™“™’«‡§¡’ §≥–·æ∑¬»“ µ√å ¡À“«‘∑¬“≈—¬‡™’¬ß„À¡à
Ú
¿“§«‘™“‡§¡’ §≥–«‘∑¬“»“ µ√å ¡À“«‘∑¬“≈—¬¡À‘¥≈

À≈—°°“√·≈–‡Àµÿº≈ ¢âÕ‡ ◊ËÕ¡‡ªìπ‚√§∑’Ëæ∫‰¥â∫àÕ¬„πºŸâ ŸßÕ“¬ÿ ·≈–‡ªì𠓇Àµÿ ”§—≠„À⺟⠟ßÕ“¬ÿ¡’§ÿ≥¿“æ™’«‘µ∑’Ë·¬à≈ß®π∂÷ß

¢—Èπæ‘°“√ Õ‘π‡µÕ√å≈‘«§‘π«—π∫’µ“ (Interleukin-1β IL-1β) §◊Õ “√ ◊ËÕÕ—°‡ ∫´÷Ë߇ªì𠓇Àµÿ ”§—≠¢Õß°“√ ≈“¬°√–¥Ÿ°ÕàÕπ
„π‚√§¢âÕ‡ ◊ËÕ¡ ‰æ≈ (Zingiber montanum (Koenig) Link ex Dietr.) ∂Ÿ°„™â„π¬“æ◊Èπ∫â“π‡æ◊ËÕ∫√√‡∑“Õ“°“√¢âÕÕ—°‡ ∫
À√◊Õª«¥¢âÕ ®÷ß π„®∑’Ë®–æ‘ Ÿ®πåƒ∑∏‘Ϫ°ªÑÕß°“√ ≈“¬°√–¥Ÿ°ÕàÕπ∑’ˇÀπ’ˬ«π”‚¥¬Õ‘π‡µÕ√å≈‘«§‘π«—π∫’µ“ ®“° “√ °—¥
·≈– “√ÕÕ°ƒ∑∏‘Ï®“°‰æ≈

«—µ∂ÿª√– ß§å ‡æ◊ËÕ»÷°…“ƒ∑∏‘Ϭ—∫¬—Èß°“√ ≈“¬°√–¥Ÿ°ÕàÕπ∑’ˇÀπ’ˬ«π”‚¥¬Õ‘π‡µÕ√å≈‘«§‘π«—π∫’µ“¢Õß “√ °—¥·≈– “√ÕÕ°

ƒ∑∏‘Ï®“°‰æ≈„πÀ≈Õ¥∑¥≈Õß

«‘∏’¥”‡π‘π°“√ ‡≈’Ȭ߰√–¥Ÿ°ÕàÕπ (cartilage explants) ∑’ˉ¥â®“°ΩÉ“‡∑â“À¡Ÿ À√◊Õ‡´≈≈å°√–¥Ÿ°ÕàÕπ„π¿“«–ª≈Õ¥‡™◊ÈÕ ‡ªìπ
‡«≈“ Ò-ÚÒ «—π„π¿“«–∑’ˉ¡à¡’À√◊Õ¡’Õ‘π‡µÕ√å≈‘«§‘π«—π∫’µ“ (ı-Úı π°./¡≈.) √à«¡°—∫ “√®“°‰æ≈Õ¬à“ß„¥Õ¬à“ßÀπ÷Ëߧ◊Õ
“√ °—¥‡Õ∑“πÕ≈, “√ °—¥‡Œ°‡´π, terpinene-4-ol ·≈– (E)-1-(3',4'-dimethoxyphenyl) butadiene (DMPBD) ∑’Ë
§«“¡‡¢â¡¢âπµà“ß Ê ‡°Á∫πÈ”‡≈’Ȭ߰√–¥Ÿ°ÕàÕπ¡“µ√«®«—¥ª√‘¡“≥ “√∫àß™’È°“√ ≈“¬°√–¥Ÿ°ÕàÕπ§◊Õ ´—≈‡øµ°≈—¬‚§´“¡‘‚π

°≈—¬·§π (s-GAG) ·≈–‰Œ¬“≈Ÿ‚√·ππ (HA) ·≈–«—¥ª√‘¡“≥ “√∑’ˇÀ≈◊ÕÕ¬Ÿà„π°√–¥Ÿ°ÕàÕπ§◊Õ §Õ≈≈“‡®π ·≈–°√¥¬Ÿ‚√π‘§
(UA) ¥â«¬«‘∏’ Dye binding assay, ELISA, Hydroxyproline assay ·≈– UA assay µ“¡≈”¥—∫ à«π‡´≈≈å°√–¥Ÿ°ÕàÕπ
π”¡“«‘‡§√“–Àå°“√· ¥ßÕÕ°¢Õ߬’π°≈ÿà¡·¡∑√‘°´å‡¡∑—≈‚≈‚ª√µ’‡π ·≈–§«“¡‡ªìπæ‘…µàÕ‡´≈≈å‚¥¬ RT-PCR ·≈– MTT
assay µ“¡≈”¥—∫

º≈°“√»÷°…“ º≈®“°°“√∑¥ Õ∫‚¥¬ cartilage explants æ∫«à“ “√ °—¥‡Œ°‡´π (Òı ¡°./¡≈.) ≈¥°“√ ≈“¬°√–¥Ÿ°

ÕàÕπ∑’ˇÀπ’ˬ«π”‚¥¬Õ‘π‡µÕ√å≈‘«§‘π«—π∫’µ“‰¥â¥’°«à“ “√ °—¥‡Õ∑“πÕ≈ ‚¥¬≈¥°“√ ≈“¬ HA, s-GAG ·≈– UA ‰¥â
√âÕ¬≈– ıÙ.Û, Ûˆ.ı ·≈– ÒÚ.¯ Õ¬à“ß¡’π—¬ ”§—≠∑’˧à“æ’ < .ı µ“¡≈”¥—∫ à«π terpinene-4-ol (Ò ‰¡‚§√‚¡≈“√å)
·≈– DMPBD (Ò ‰¡‚§√‚¡≈“√å) ≈¥°“√ ≈“¬°√–¥Ÿ°ÕàÕπ‰¥âÕ¬à“ß¡’π—¬ ”§—≠ (§à“æ’ < .ı) ·≈–¥’°«à“ “√ °—¥∑—Èß
Õß™π‘¥ ·µà DMPBD ‡ªìπ¡‘µ√µàÕ‡´≈≈å°√–¥Ÿ°ÕàÕπ¡“°°«à“ terpinene-4-ol ¬—ßæ∫«à“ DMPBD (Ò ‰¡‚§√‚¡≈“√å)
¬—∫¬—Èß°“√· ¥ßÕÕ°„π√–¥—∫¬’π¢Õ߇ÕÁπ‰´¡å∑”≈“¬°√–¥Ÿ°ÕàÕπ §◊Õ·¡∑√‘°´å‡¡∑—≈‚≈‚ª√µ’‡π -ÒÛ ‰¥â√âÕ¬≈– Ú˜.ı

‚¥¬¡’π—¬ ”§—≠∑’˧à“æ’ < .ı

¢âÕ √ÿª °“√∑¥≈Õߧ√—Èßπ’È∂◊Õ‡ªìπ°“√√“¬ß“π§√—Èß·√°∂÷ߺ≈¢Õß “√ °—¥·≈– “√ÕÕ°ƒ∑∏‘Ï®“°‰æ≈ „π°“√¬—∫¬—Èß°“√°“√
≈“¬°√–¥Ÿ°ÕàÕπ∑’ˇÀπ’ˬ«π”‚¥¬Õ‘π‡µÕ√å≈‘«§‘π«—π∫’µ“ ∑—Èß„π cartilage explant ·≈–„π√–¥—∫¬’π¢Õ߇´≈≈å°√–¥Ÿ°ÕàÕπ
‰æ≈®÷ß¡’ª√– ‘∑∏‘¿“æ‚¥¥‡¥àπ‡À¡“– ”À√—∫æ—≤𓇪ìπ¬“√—°…“‚√§¢âÕ‡ ◊ËÕ¡Õ¬à“ß®√‘ß®—ßµàÕ‰ª