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632 วารสารการแพทย์แผนไทยและการแพทย์ ทางเลือก ปีที่ 21 ฉบับที่ 3 กันยายน-ธันวาคม 2566

For alpha-amylase, Kratom extracts were compounds may play a significant role in this
not effective on this enzyme indicating that activity. Therefore, further research, such as

Kratom was unable to inhibit the conversion bioassay-guided fractionation, is necessary
of starch into soluble polysaccharides or disac- to identify additional responsive markers for
charides. As well as ACE, Kratom extracts did quality control purposes.

not exert inhibition of ACE, suggesting that This study was limited to explore only the
Kratom is unlikely to be effective in reduc- in vitro enzymatic-based assays which require
ing hypertension through this mechanism. additional in vitro and in vivo assay models to

In contrast, there has been report of Kratom conclude the possibility of using Kratom as a
intake leading to cardiovascular side effects, medicine for patients or as dietary supplement.
[34]
including tachycardia and hypertension.
Patients should be advised for caution when Conclusion
considering Kratom intake. Kratom leaves have long been utilized

Regarding the content of mitragynine, as traditional and folk medicines in Thailand.
KE95 exhibited the highest concentration of After it became legal, the widespread use of
mitragynine, while KDec displayed the lowest. Kratom based on personal belief raised caution

The mitragynine content varies depending among certain patients. This study aimed to
on the polarity of the extraction solvent. The investigate the pharmacological potential of

inhibitory activity on BuChE tended to cor- Kratom using enzymatic-based assays. The
relate with the mitragynine content. A higher results of this study revealed that Kratom
concentration of mitragynine demonstrated extracts demonstrated the most potential as

greater potency in inhibiting BuChE, whereas BuChE inhibitors, which is relevant to anti-Al-
a lower concentration of mitragynine tended to zheimer’s disease treatment. As anti-diabetes,
result in decreased inhibitory activity. These Kratom exhibited moderate activity in inhib-

findings suggest that mitragynine may be iting alpha-glucosidase but did not inhibit
associated with BuChE inhibition, however, alpha-amylase. Additionally, Kratom extracts
other indole alkaloids should also be deter- did not demonstrate anti-hypertension effects

mined. In contrast to a-glucosidase, KE70 and as ACE inhibitor, instead, its side effects on
KE50 exhibited the highest inhibitory activity. tachycardia and hypertension should be cau-

However, these results were not linked to the tioned. This study is the first to report on the
mitragynine content, indicating that other inhibitory effects of Kratom extracts on BuChE,