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630 วารสารการแพทย์แผนไทยและการแพทย์ ทางเลือก ปีที่ 21 ฉบับที่ 3 กันยายน-ธันวาคม 2566

Figure 4 Overlay spectrum of mitragynine peak in sample solution and standard solution (A) and calibration
curve of mitragynine at various concentrations (B).

Discussion tom ethanolic and aqueous extracts on AChE

Kratom has a long and rich history of use and BuChE were reported for the first time,
in Thailand particularly as an ingredient in indicating that Kratom potentially be used in

TTM remedies, but it has never been utilized Alzheimer’s disease. AChE and BuChE inhibi-
as single herb in TTM practice. However, local tors are crucial targets for drugs used in treat-
folk medicine, especially in southern Thailand, ing Alzheimer’s patients since they inhibit

has a tradition of chewing Kratom leaves for acetylcholine turnover, leading to restore the
variety of reasons, including the treatment of synaptic level of acetylcholine in patients. [28]
diabetes, hypertension, and even to boost work Innok and their colleagues have conducted a

performance . The objective of this study was study on the inhibitory activity of AChE using
[7]
to investigate the enzymatic-based biological molecular docking and confirmed with an
activities related to anti-diabetes, anti-hyper- in vitro experiment. They found that mitragy-

tension, and anti-Alzheimer’s disease based nine, which is a main alkaloid in Kratom leaves
on both traditional medicine and the folk us- extract exhibited a good binding affinity

age of Kratom leaves, in order to support the towards AChE. Their experiment also revealed
legislative intention of unlocking its use as an that the methanolic Kratom extract and mitra-
herbal or traditional medicine. gynine exerted in vitro anti-AChE activities

In this study, the inhibitory effects of Kra- with IC 50 values of 252.68 µg/mL and 105.12