Page 146 - Acrobat J Trad-21-3-2566

J Thai Trad Alt Med Vol. 21 No. 3 Sep-Dec 2023 629

In summary, all extracts exhibited in- sample solution was 26.3 minute (Figure 3) and
hibitory effects on AChE and BuChE enzymes, UV spectrum of mitragynine peak in sample

with KE95 demonstrating the most significant solution corresponded to the peak of standard
effect on BuChE. The KE70 and KE50 also solution (Figure 4a). The linearity of calibra-
exerted moderate inhibition of alpha-glucosi- tion curve determining by the coefficient of

dase, while aqueous extract (KDec) exhibited determination (r ) was 0.9997 (Figure 4b) and
2
low inhibitory activity on ACE. All extracts the recovery of the QC samples ranged from
were found to be ineffective in inhibiting 97.26% to 102.34%. The content of mitragynine

alpha-amylase and ACE enzymes. in KE95, KE70, KE50 and KDec samples was
determined to be 54.00 ± 1.17, 35.14 ± 0.64,
4. Content of mitragynine 25.05 ± 0.05, and 18.08 ± 0.30 mg/g, respec-

The retention time of mitragynine in tively.

Figure 3 Chromatogram of diluent (methanol) (A), mitragynine standard solution (B) and sample solution (C).