Page 121 - วารสารการแพทย์แผนไทย ปีที่ 21 ฉบับที่ 2

J Thai Trad Alt Med Vol. 21 No. 2 May-Aug 2023 337

Gene expression assay of four main at the doses of 1,000 and 1,500 µg/mL, but
genes for viral entry and progression were not in dose-response manner. In contrast, the

analysed. Lung cells were treated with CTL at expression of cathepsin L was up-regulated
concentrations of 1000, 1500 and 2000 µg/mL. at the doses of 1,000 and 1,500 µg/mL. The
ACE2 gene expression was down-regulated mRNA expression data of ACE2, TMPRSS2,

compared to the untreated control and dem- PIKfyve and cathepsin L in calu-3 cells treated
onstrated a dose-response relationship. TM- with different concentrations of CTL are shown
PRSS2 and PIKfyve demonstrated inhibition in Figure 5.

Figure 5 Relative quantity of mRNA expression of ACE2, TMPRSS2, PIKfyve and cathepsin L in calu-3 cells
treated with CTL at 1,000, 1,500 and 2,000 µg/mL versus control data set for 24 h. Data is ex-
pressed as mean ± SD, n = 3. Statistical analysis was carried out using One-Way analysis of variance
followed by Kruskal-Wallis multiple comparisons (*p < 0.5 and **p < 0.01).