Page 120 - วารสารการแพทย์แผนไทย ปีที่ 21 ฉบับที่ 2

336 วารสารการแพทย์แผนไทยและการแพทย์ ทางเลือก ปีที่ 21 ฉบับที่ 2 พฤษภาคม-สิงหาคม 2566

The cytotoxicity of CTL, PSP and Y5R between IC 10 and IC 20 values. CTL demon-
were evaluated using MTT assay. The non- strated inhibition in a dose dependant manner

linear regression was generated between log whereas, PSP and Y5R showed lower inhibitory
concentration and % viability. PSP gave the activity (roughly 15-20% at the concentration
inhibitory concentration (IC) at 50% (IC 50) 20% of 25 and 50 µg/mL). CTL also inhibited TM-

(IC 20) and 10% (IC 10) of 42695.72, 346.96 and PRSS2 enzyme in a dose dependent manner
6.50 µg/mL, respectively. Y5R gave the IC 50, (p < 0.5). The % relative inhibition was 53.82,
IC 20 and IC 10 of 14068.70, 453.74 and 49.56 µg/ 63.90 and 71.15 % at the doses of 1,000, 1,500

mL, respectively. Whereas, CLT gave the lower and 2,000 µg/mL, respectively. ACE2 and
cytotoxicity at the IC 50, IC 20 and IC 10 of 4597.27, TMPRSS2 enzymatic inhibition at different
1995.50 and 1377.86 µg/mL, respectively. concentrations of PSP, Y5R and CTL were

ACE2 enzymatic inhibition of the extracts shown in Figure 3 and Figure 4.
was evaluated at approximate concentrations

Figure 3 ACE2 enzymatic inhibitory activity at the Figure 4 TMPRSS2 enzymatic inhibitory activity at the
highest concentrations which were not highest concentrations which were not toxic
toxic to calu-3 cells of MLN4760 standard to calu-3 cells of camostat standard inhibi-
inhibitor, PSP, Y5R and CTL. Data is ex- tor, PSP, Y5R and CTL. Data is expressed
pressed as mean ± SD, n = 3. Statistical as mean ± SD, n = 3. Statistical analysis
analysis was carried out using One-Way was carried out using One-Way analysis of
analysis of variance (non-parametric test) variance followed by Kruskal-Wallis multiple
followed by Kruskal-Wallis multiple com- comparisons (*p < 0.5).
parisons (**p < 0.01 and ***p < 0.001).