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114 วารสารการแพทย์แผนไทยและการแพทย์ ทางเลือก ปีที่ 20 ฉบับที่ 1 มกราคม-เมษายน 2565

from red blood cells. Enhanced susceptibility The treatment of erythrocytes with hy-
against oxidative stress in G6PD deficient drogen peroxide resulted in the rupture of cell
erythrocytes is caused by ingestion of fava membranes and caused visual hemolysis. This

beans, infection, or exposure to an oxida- study demonstrates that concomitant admin-
tive drug [8,21-22] . Faba bean (Vicia faba L.) is a istration L. leucocephala young leave extract

leguminous plant belonging to the Fabaceae and oxidating agent can significantly decrease
family. Faba beans are rich in two glucosidic the oxidative hemolysis in G6PD deficiency
aminopyrimidine derivatives, vicine, and con- erythrocytes in comparison with negative

vicine, and their respective aglycones, divicine control. The obtained results confirmed L. leu-
and isouramil . Divicine and isouramil have cocephala extract possesses a dose-dependent
[23]
been identified as the main factors of G6PD protective capacity in peroxidation conditions
deficient , which was not found in L. leuco- and decreases the Heinz body formation in
[24]
cephala leaves extract. The phytochemical in- G6PD deficiency erythrocytes.

vestigation of L. leucocephala leaves revealed It was reported that medicinal plants
the presence of terpenes, sterols, coumarins containing phenolic and flavonoid compounds
and 8 flavonoids including quercetin-3-O- possess antimicrobial activity [23-28] . In disk

(2¢¢-trans-p-coumaryl)-α-rhamnopyranosyl- diffusion test, clinical strains of bacteria are
(1¢¢¢→6¢¢)-b-glucopyranoside (1), quercetin- not sensitive to L. leucocephala young leave
3-O-α-rhamnopyranosyl-(1¢¢¢ →2¢¢)- extract. Despite the previous study showed

b–glucopyranoside (2), quercetin-7-O-α– significant antimicrobial activity against E.
rhamnopyranosyl-(1¢¢¢→2¢¢)-b-glucopyranoside coli, Pseudomonas, and Salmonella typhimuri-

(3), quercetin-3-O-α-rhamnopyranoside (4), um, moderate activity against gram positive
quercetin-3-O-b–glucopyranoside (5), isovi- bacteria . Because of efficacy of extract var-
[29]
texin (6), vitexin (7) and quercetin (8) . Other ies depending on the microbial species and
[6,25]
studies on the leave extracts had shown an- strains. The bacterial properties of American
tioxidant activity [25-26] . In the present study, Type Culture Collection (ATCC) have different

when G6PD-deficient erythrocytes were from clinical strains. The relation between
incubated with the extract in the presence bacterial strain and composition of S. aureus
of APH, the extract showed activity in sig- has been reported . Our finding also implies
[30]
nificantly reducing the Heinz body formation poor activity of L. leucocephala young leave
was noticed at the concentrations 100, 500, extract against clinical isolation stain.
and 1,000 μg/mL. It is very well known that all parts of