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J Thai Trad Alt Med Vol. 20 No. 1 Jan-Apr 2022 113

5. Effects of the extracts on viability of extract than Molt4 cell. The IC of extract on
20
leukemic cell line KG-1a cell was 79.2 ± 0.5 μg/mL. No cytotoxic
The cytotoxic effect of L. leucocephala effect was obtained with Molt4 leukemic cells

young leave extract on leukemic cell lines compared to vehicle control. The cell inhibition
was investigated by MTT assay. The result activity showed in Figure 2.

revealed that KG-1a cell was more sensitive to

Figure 2 Cytotoxic effect of L. leucocephala young leave extracts on (A) KG-1a and (B) Molt4 cell line. The
average of cell viability was obtained from three independent experiments.

Discussion radicals cause oxidative stress and damage

L. leucocephala leaves extract contains cell membrane [18-19] . In general, the activity of

high condensed tannins, terpenes, sterols, the extract in the protection of erythrocyte
coumarins, and flavonoids . The result of membrane is due to its ability to act as antioxi-
[6]
the total phenolic content determination test dant. Recently, phenolic compounds including

showed that the extract was 325.6 ± 0.7 mg gal- tannins, phenolic acids and flavonoids have
lic acid equivalent/g of dry extract (mg GAE/g been well recognized as antioxidants .
[20]
extract). The antioxidant properties of extract G6PD enzyme is rate-limiting enzyme
from L. leucocephala found in this study are in pentose phosphate pathway (PPP) that is
mainly due to the phenolic content present in responsible for nicotinamide adenine dinucleo-

the extract. tide phosphate (NADPH) production. NADPH
Reactive oxygen species (ROS) includ- is necessary for maintaining glutathione
ing hydrogen peroxide, superoxides, hydroxyl in reduced form to remove oxidative stress