Page 125 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 20 ฉบับที่ 1

วารสารการแพทย์แผนไทยและการแพทย์ ทางเลือก Journal of Thai Traditional & Alternative Medicine
ปีที่ 20 ฉบับที่ 1 มกราคม-เมษายน 2565 Vol. 20 No. 1 January-April 2022

นิพนธ์ต้นฉบับ

Bioactivities of Leucaena leucocephala Young Leaf Crude Extract

Rungkarn Sangkaruk , Pawaret Panyajai †
*,‡
Department of Clinical Hematology, Microscopy and Parasitology, Faculty of Medical Technology, Western University,
*
Sa Long Ruea Subdistrict, Huai Krachao District, Kanchanaburi 71170, Thailand.
Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Sripoom Subdistrict,
†
Mueang District, Chiang Mai 50200, Thailand.
‡ Corresponding author: rungkarn.sa@western.ac.th

Abstract

Leucaena leucocephala (krathin in Thai) has been used in foods and traditional medicines for a long time.
This study aimed to assess L. leucocephala young leaf extract for determining its total phenolic content, investigate
the inhibition of Heinz body formation, hemolytic effect and protection of G6PD-deficient human erythrocytes
against oxidative damage, antimicrobial activity, and cytotoxicity on leukemic cell lines. The results demonstrated
that the total phenolic content of L. leucocephala young leaf extract was 325.6 ± 0.7 mg gallic acid equivalent
per gram of dried extract (mg GAE/g extract). The extract could inhibit the Heinz body formation induced by
acetylphenylhydrazine (APH) and prevent hemolysis damage induced by hydrogen peroxide in a concentration-
dependent manner. The highest inhibition of Heinz body formation and protection values were about 12.8% and
4.3%, respectively, at the concentration 1,000 g/mL for G6PD-deficient erythrocytes. Regarding toxicity, KG-1a
cell was more sensitive to the extract than Molt4 cell. The IC20 of extract on KG-1a cell was 79.2 ± 0.5 μg/mL,
but the extract had no antimicrobial effect on bacteria from clinical samples. It is thus suggested that further in vivo
studies should conducted on the effect of L. leucocephala young leaf extract to determine its non-toxic therapeutic
doses for preventing oxidative stress and hemolysis in G6PD-deficient erythrocytes.

Key words: Leucaena leucocephala, G6PD-deficiency, antioxidative activity, Heinz bodies’ formation,
leukemia

Received date 05/07/21; Revised date 29/01/22; Accepted date 31/03/22

105