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504 วารสารการแพทย์แผนไทยและการแพทย์ ทางเลือก ปีที่ 19 ฉบับที่ 2 พฤษภาคม-สิงหาคม 2564

Table 2 Composition of formulation 2

Formulation 2 Quantity (gm) Function
Part A
Cetostearyl alcohol 25 stiffening agent
Tween 60 2.5 emulsifier
Span 80 2.5 emulsifier

Part B
Disodium edetate 0.1 chelating agent
Sodium metabisulfite 0.1 antioxidant
Water qs to 91.0 diluent
Part C
secondary Clinacanthus nutans ethanolic extract. 4.0 active
Tween 20 5.0 emulsifier

Total weight 100.0

limit are not less than 1.0 percent. The total 2.3 Stability evaluation
flavonoids of Clinacanthus nutans creams are The Stability carries out in Ambient

not less than 10 mg%. condition and the products are filled in Alu-
2.2 Determination of total flavonoids minium Tube 5 gm. The stability evaluation of
Leaf extract or cream of Clinacanthus the 4% primary Clinacanthus nutans ethanolic

nutans (1 mL) was added to 10-ml volume- extract cream (Formulation 1) compare to 4%
tric flasks containing 4 ml methanol. NaNO 2 secondary Clinacanthus nutans (Formulation
solution (1:5, w/v, 0.3 mL) was added to the 2) ethanolic extract cream as follows

flasks and allowed to stand for 6 min at room
temperature. After 6 min, 0.3 ml AlCl3 solution Results

(1:10, w/v) was added to the flasks, mixed well Clincanthus nutans is an Asean herb.
and kept for 6 min at room temperature. At Roots contain betulin, lupeol, b-sitosterol, and
last 2.0 ml of 1M NaOH solution was added stigmasterol. The leaves contain lupeol, b-

and held for 10 min at room temperature. The sitosterol, stigmasterol, phenolic acids and
absorbance was measured at 510 nm by spec- flavonoid compound . It demonstrated
[12]
trophotometer (Shimadzu, Japan). Quercetin good free radical scavenging activity when
was used as a standard for the calibration measured by different methods. Clincanthus
curve. nutans should be harvested 6 months after