Page 273 - วารสารการแพทย์แผนไทยและการแพทย์ ทางเลือก ปีที่ 19 ฉบับที่ 2 พฤษภาคม-สิงหาคม 2564

J Thai Trad Alt Med Vol. 19 No. 2 May-Aug 2021 503

leaves of Clinacanthus nutans were extracted until they reach 72˚C. Pour part A to part B, stir
by using 1.0 L of 95% ethanol as a solvent and until the mixture become homogeneous and

regularly shaken 3-4 times a day for 7 days. then cool down to 42˚C to be ready for adding
The ethanolic extract of Clinacanthus nutans Part C the primary or secondary Clinacanthus
leaves was filtered and concentrated on the nutans ethanolic extract.

water bath under controlled temperature 1.4 Preparation of the 4% Clinacanthus
60-70˚C until the final weight of the primary nutans ethanolic extract cream - Pour the 4 gm
Clinacanthus nutans ethanolic extract is 4 gm. of primary or secondary Clinacanthus nutans

and stored at 4˚C in a refrigerator till further ethanolic extract into 42˚C cream base and
use. Preparation of Secondary Clinacanthus stir until the mixture become homogenous.
nutans-ethanolic extract - The 100 gm of pri- The two preparations were used to compare

mary Clinacanthus nutans ethanolic extract the stability regarding to the physicochemical
was added with Tween- 20, 5 gm and stirred properties, pH value and % label amount of

until homogeneous. total flavonoids. [12]
1.3 Preparation of cream base - The 100
gm. cream base formulation 1 and 2 are as 2. Methods

follows 2.1 Quantitative phytochemical analysis
1.3.1 Procedure The total flavonoids of the ethanolic ex-

Heat part A and part B on a water bath tract of the fresh leaves of Clinacanthus nutans

Table 1 Composition of formulation 1

Formulation 1 Quantity (gm) Function

Part A
Cetostearyl alcohol 25 stiffening agent
Tween 60 2.5 emulsifier
Span 80 2.5 emulsifier
Part B
Disodium edetate 0.1 chelating agent
Sodium metabisulfite 0.1 antioxidant
Water qs to 96.0 diluent

Part C
primary Clinacanthus nutans ethanolic extract. 4.0 active
Total weight 100.0