Page 60 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 10 ฉบับที่ 2 เสริม

Journal of Thai Traditional & Alternative Medicine Vol. 10 No. 2 May-August (Supplement) 2012 51

PC-4

°“√»÷°…“°“√ª≈¥ª≈àÕ¬ “√ compound D ®“°º≈‘μ¿—≥±å„π√Ÿª·∫∫‰¡‚§√

Õ‘¡—≈™—π¢Õß “√ °—¥‰æ≈

1 2 1
√¡¬å°¡≈ «‘®‘μ√®—π∑√å , √—∞æ≈ Õ“…“ ÿ®√‘μ , ª√– “π μ—È߬◊π¬ß«—≤π“
1
§≥–°“√·æ∑¬å·ºπμ–«—πÕÕ° ¡À“«‘∑¬“≈—¬√—ß ‘μ
2
“¢“«‘™“‡∑§‚π‚≈¬’‡¿ —™°√√¡ §≥–‡¿ —™»“ μ√å ¡À“«‘∑¬“≈—¬»√’π§√‘π∑√«‘‚√≤

À≈—°°“√·≈–‡Àμÿº≈ compound D ‡ªìπ “√ ”§—≠™π‘¥Àπ÷Ëß∑’ËÕ¬Ÿà„π “√ °—¥‰æ≈ ¡’ƒ∑∏‘Ï„π°“√μâ“π°“√

Õ—°‡ ∫ ´÷Ë߇ªìπ¢âÕ∫àß„™â∑’Ë ”§—≠¢Õ߉æ≈ °“√‡μ√’¬¡ “√ °—¥‰æ≈„π√Ÿª·∫∫‰¡‚§√Õ‘¡—≈™—𠇪ìπ°“√ª√–¬ÿ°μå„™â
‡∑§π‘§„π°“√≈¥¢π“¥Õπÿ¿“§‡æ◊ËÕ‡æ‘Ë¡°“√¥Ÿ¥´÷¡¢Õß “√ °—¥ºà“π‡¢â“ Ÿàº‘«Àπ—ß ·≈–‰ª¬—ß∫√‘‡«≥∑’Ë¡’°“√Õ—°‡ ∫
‡™àπ‡ âπ‡ÕÁπ ·≈–°≈â“¡‡π◊ÈÕ‰¥â‡√Á«¬‘Ëߢ÷Èπ „π°“√‡μ√’¬¡‰¡‚§√Õ‘¡—≈™—π‰¥â¡’°“√∑¥ Õ∫°“√ª≈¥ª≈àÕ¬ “√ ”§—≠
‚¥¬„™â‡§√◊ËÕß Franz diffusion cell ‡ªìπÕÿª°√≥å„π°“√«‘‡§√“–Àå§à“ —¡ª√– ‘∑∏‘°“√´÷¡ºà“π ‡æ◊ËÕ‡ªìπ°“√
§«∫§ÿ¡§ÿ≥¿“æ¢Õߺ≈‘μ¿—≥±å‰¡‚§√Õ‘¡—≈™—π
«—μ∂ÿª√– ß§å »÷°…“°“√ª≈¥ª≈àÕ¬ “√ compound D ®“°º≈‘μ¿—≥±å„π√Ÿª·∫∫‰¡‚§√Õ‘¡—≈™—π¢Õß

“√ °—¥‰æ≈‚¥¬„™â‡§√◊ËÕß Franz diffusion cell
«‘∏’¥”‡π‘π°“√ ‡μ√’¬¡ “√ °—¥‰æ≈‚¥¬„™âºß·Àâß 1.00 °°. À¡—°°—∫ 95% ‡Õ∏“πÕ≈®”π«π 3 ≈‘μ√‡ªìπ
‡«≈“ 7 «—π π”¡“°√Õß·≈–√–‡À¬„Àâ·Àâߥ⫬ rotary evaporator ‰¥â “√ °—¥ 0.14 °°. °“√‡μ√’¬¡‰¡‚§√
Õ‘¡—≈™—π®–„™â 1% ¢Õß “√ °—¥‰æ≈„πÕߧåª√–°Õ∫¢Õß «—Ø¿“§πÈ”¡—π/ “√°àÕÕ‘¡—≈™—π/πÈ” ‡ªìπ peppermint
oil/ Tween 20/ water „πÕ—μ√“ à«π 2 : 5 : 3 ®“°π—Èπ𔉪«—¥¢π“¥Õπÿ¿“§ ·≈–§à“»—°¬å‰øøÑ“´’μ“¥â«¬‡§√◊ËÕß
zetasizer model S4700 (Malvern, UK) ‡§√◊ËÕß Franz diffusion cell ´÷Ëß∫√√®ÿ phosphate buffer pH 7.4
ªî¥°—Èπ√–À«à“ß donor ·≈– receptor part ¥â«¬·ºàπ cellulose acetate membrane ‡μ‘¡‰¡‚§√Õ‘¡—≈™—π
¢Õß “√ °—¥‰æ≈ª√‘¡“μ√ 3 ¡≈. „π donor part ·≈â«∑”°“√‡°Á∫μ—«Õ¬à“ß “√≈–≈“¬®“° receptor part ª√‘¡“μ√
5 ¡≈. ∑’ˇ«≈“ 10, 30, 60, 120 ·≈– 180 π“∑’ ®“°π—È𫑇§√“–ÀåÀ“ª√‘¡“≥ compound D ∑’˪≈¥ª≈àÕ¬ÕÕ°

¡“¥â«¬‡§√◊ËÕß HPTLC (Camag, Switzerland) π”§à“∑’ˉ¥â¡“ √â“ß°√“øª√‘¡“≥ “√ ”§—≠∑’˪≈¥ª≈àÕ¬ÕÕ°
¡“ – ¡ (cumulative amount) °—∫ square root time ·≈–§”π«≥§à“ diffusion coefficient (D)
μ“¡ ¡°“√¢Õß Higuchi
º≈°“√»÷°…“ æ∫«à“‰¡‚§√Õ‘¡—≈™—π∑’˪√–°Õ∫¥â«¬ peppermint oil/ Tween 20/ water „πÕ—μ√“ à«π
2 : 5 : 3 ¡’¢π“¥Õπÿ¿“§‡©≈’ˬ 40 ± 1.0 π“‚π‡¡μ√ ·≈–¡’§à“»—°¬å‰øøÑ“´’쓇∑à“°—∫ -5.6 ± 0.41 ¡‘≈≈‘‚«≈μå
-4
§à“ —¡ª√– ‘∑∏‘°“√·æ√à¢Õß compound D ¢Õßμ”√—∫‰¡‚§√Õ‘¡—≈™—π‰æ≈π’È¡’§à“‡∑à“°—∫ 3.89 x 10 ± 2.15 x
-5
10 ´¡2/π“∑’ · ¥ß„Àâ‡ÀÁπ«à“ compound D “¡“√∂∂Ÿ°ª≈¥ª≈àÕ¬ÕÕ°¡“‰¥â
¢âÕ √ÿª ‰¡‚§√Õ‘¡—≈™—π¢Õß “√ °—¥‰æ≈∑’ˇμ√’¬¡¢÷Èπ‚¥¬„™â√–∫∫ peppermint oil/ Tween 20/ water
„πÕ—μ√“ à«π 2 : 5 : 3 ¡’¢π“¥Õπÿ¿“§∑’ˇ≈Á°„π√–¥—∫π“‚π‡¡μ√ ·≈–®“°°“√∑¥ Õ∫°“√ª≈¥ª≈àÕ¬ “√ com-
pound D ¥â«¬ Franz diffusion cell æ∫«à“ “√ ”§—≠ “¡“√∂∂Ÿ°ª≈¥ª≈àÕ¬ÕÕ°¡“‰¥â ‰¡‚§√Õ‘¡—≈™—π¢Õß
“√ °—¥‰æ≈¥—ß°≈à“«¡’»—°¬¿“æ„π°“√æ—≤𓉪‡ªìπ¬“μâ“π°“√Õ—°‡ ∫∑’Ë„™â¿“¬πÕ°