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Journal of Thai Traditional & Alternative Medicine Vol. 8 Nos. 2-3 May-December 2010 Ú˘

A B C

Fig. 7 Flowers of A. sinensis (Oliv.) Diels growing in a nursery. A. budding; B. young flowers; C. blooming flower.

ture room was 100 per cent after one month and two Acknowledgements
months, compared with those grown in the nursery,
The author would like to thank Mrs. Pranee
which were 83.5 and 72.6 percent, respectively (Table
Chavalittumrong, Medical Scientist Advisory Level
3). The stems and roots of those grown in the tissue
(Physical Sciences), Department of Medical Sciences,
culture room were taller and bigger when compared
Ministry of Public Health, for kindly collecting rhi-
with those grown in the nursery, (Figs. 4-5). The young
zomes of A. sinensis from the Kunming Institute of
plants were successfully transferred into soil and had
Botany, China, for us to study tissue cultures of A.
flowers (Figs. 6-7).
sinensis.
Discussion
References
Our experiment used rhizomal buds which in- 1. Andrew Chevallier. The encyclopedia of medicinal plants. 1996.
duced multiple shoots. The value of this mass propa- 2. Qi-bing M, Jing-yi T, BoC. Advance in the pharmacological studies of
radix Angelica sinensis (Oliv.) Diels (Chinese dang gui). Chin Med J
gation method was that it produced no abnormal plant-
1991;104:776-81.
5
lets, no immature embryo-derived cultured cells. The
3. Foster S, Yue CX. Herbal Emissaries. Rochester, VT: Healing Arts
plantlets grown in the tissue culture room fared bet- Press; 1992. p. 65-72.
4. Hirata JD, Swiersz L M, Zell B, et al. Does dong quai have estrogenic
ter than those grown in the nursery: they had taller
effects in postmenopausal women? double-blind, placebo-controlled
stems, bigger roots and a higher rate of survival be-
trial. Fertile Sterile 1997;68:981-6.
cause the nature of these plants is to grow in a cold 5. Tsay HS, Huang HL. Somatic embryo formation and germination from
climate (5.5-11.4˚C) and on high mountains (1,500- immature embryo-derived suspension-cultured cells of Angelica sinensis
8 (Oliv.) Diels. Plant Cell Reports 1998;17:670-4.
3,000 m). These young plants were successfully
6. Huang WH, Song CQ. Studies on the chemical constituents of Angelica
transferred into soil and flowered in the nursery. The sinensis. Yao-Xue-Xue-Bao 2003;38(9):680-3.
study shows that Thailand could successfully plant 7. Murasige T, Skoog T. A revised medium for rapid growth bioassays
with tobacco tissue cultures. Physiol Plantarum 1962;15:473-97.
dang gui if growing this plant on high mountains and
8. Shi-Yu Z. and Kuo-Chang C. Angelica sinensis (Oliv.) Diels: In vitro
in a cold climate such as Doi Angkhang in Chiang culture, regeneration, and the production of medicinal compounds.
Mai Province. Biotech Agri Forest 1989;7:1-22.