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Materials and methods Results
Rhizomal buds of A. sinensis (Oliv.) Diels were The best media for multiple shoot induction was
collected from a field in Kunming Institute of Botany, MS containing 3 mg/l BAP and 1 mg/l NAA, which
China. Rhizomal buds were of sterilized with half a produced an average of 3.45 shoots per culture and

spoonful of orthocide in 100 ml of sterilized water for an average shoot length of 3.23 cm in one month
30 minuetes, washed with 10% and 5% Clorox, con- (Table 1, Fig. 1). The best rooting medium contained
taining 1-2 drops of Tween 20 for 10 and 5 min, re- 0.1 mg/l KN and 0.02 mg/l NAA (Table 2, Figs. 2-3),
spectively, and finally rinsed 2-3 times with sterilized the average root induction of which was 100 percent
water. The buds were removed aseptically from the for one month and two months, and the average num-
rhizomes and cultured onto Murashige and Skoog (MS) ber of roots 1.85 and 7.40 shoots for one month and
basal medium. After one month, shoot tips induced two months, respectively. The average root length,

from the buds were subcultured onto MS containing average shoot length, average number of shoots were
3 mg/l benzyl amino purine (BAP or B) and 0.1 or 1 1.78 cm, 4.15 cm and 1.05 for one month, respec-
mg/l naphthalene acetic acid (NAA), and MS58 (0.1 tively. The average root length, average shoot length
mg/l BAP, 5 mg/l giberellic acid (GA), 150 mg/l citric and average number of shoots were 4.78 cm, 8.15 cm
acid and double the strength of iron for the induction and 2.95, respectively, for two months. The percent-
of multiple shoots for one month. The shootlets were age of survival for plantlets grown in the tissue cul-
subcultured in rooting media, which were MS con-
taining 0.2 mg/l BAP and 2 mg/l 3-indole acetic acid
(IAA), 0.1 mg/l kinetin (KN) and 0.02 mg/l NAA and Table 1 Average number of shoots, average shoot length of A.

filter paper moistened with 1 MS liquid medium. The sinensis (Oliv.) Diels after culturing shoot tips on MS
medium containing 3 mg/l BAP and 0.1 mg/l NAA, 3
plantlets were those grown in a tissue culture room
mg/l BAP and 1 mg/l NAA and MS58 for one month.
(controlled temperature = 25˚C, with 16 hours of light)
compared with those grown in a nursery. The me- Multiple shoots
dium for growing in the tissue culture room using Hormone Average number Average shoot
of shoots length (cm)
sterilized vermiculite in pots and the medium for grow-
BAP3NAA0.1 2.55 3.2
ing in the nursery using sterilized soil : ash : fertilizer
BAP3NAA1 3.45 3.23
in the ratio of 2:2:1 in black plastic packets.
MS58 1.25 5.40
Note: MS58 = MS medium containing 0.1 mg/l BAP,5 mg/l GA, 150 mg/
l citric acid and double the strength of iron.

Table 2 Average percentage of root induction, average number of roots, average root length, average shoot length and average
number of shoots of A. sinensis (Oliv.) Diels after culturing shootlets on MS medium containing 0.2 mg/l BAP and 2 mg/l
IAA, 0.1 mg/l KN and 0.02 mg/l NAA and 1 MS liquid for 1 month and 2 months

Average% of root Average number Average root Average shoot Average number
Hormone induction of roots length (cm) length (cm) of shoots

1M 2M 1M 2M 1M 2M 1M 2M 1M 2M
BAP0.2IAA2 20 95 0.35 3.6 0.11 3.64 4.6 6.18 2.6 3.05
KN0.1NAA0.02 100 100 1.85 7.40 1.78 4.78 4.15 8.15 1.05 2.95
1 MS liquid 0.05 100 0.05 3.20 0.03\ 3.08 3.15 5.14 1.30 1.70

Note: M = month; 1 MS liquid = filter paper moistened with 1 MS liquid.