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° Journal of Thai Traditional & Alternative Medicine

ªï∑’Ë ¯ ©∫—∫∑’Ë Ú-Û æƒ…¿“§¡-∏—𫓧¡ ÚııÛ Vol. 8 Nos. 2-3 May-December 2010

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Studies on Tissue Culture of Angelica sinensis (Oliv.)
Diels

Thanyawan Mongkolchaipak*
Papavadee Suchantaboot*

Abstract
Rhizomal buds of Angelica sinensis (Oliv.) Diels were collected from a field in Kunming Institute of
Botany, China. Rhizomal buds were sterilized with orthocide and Clorox. The buds were removed aseptically
from the rhizome and cultured onto Murashige and Skoog (MS) basal medium. After one month, shoot tips of
plantlets induced from the buds were subcultured onto MS containing 3 mg/l benzyl amino purine (BAP or B)
and 0.1 or 1 mg/l naphthalene acetic acid (NAA), and MS58 (0.1 mg/l BAP, 5 mg/l giberellic acid, 150 mg/l
citric acid and double the strength of iron) for multiple shoot induction. The best media for multiple shoot
induction were MS containing 3 mg/l BAP and 1 mg/l NAA which had an average of 3.45 shoots per culture in
one month. The rooting medium was MS containing 0.2 mg/l BAP and 2 mg/l 3-indoleacetic acid (IAA), 0.1
mg/l kinetin (KN) and 0.02 mg/l NAA, and filter paper moistened with 1 MS liquid medium. The result of the
best rooting medium was 0.1 mg/l KN and 0.02 mg/l NAA which became plantlets. The percentage of survival
for plantlets grown in a tissue culture room (controlled temperature = 25˚C, with 16 hours of light) was 100
per cent after one and two months, compared with those grown in a nursery, which were 83.5 and 72.6 per-
cent, respectively.
Key words: Angelica sinensis, tissue culture

Introduction 2
of menstruation; it relieves an abnormal urinary sys-
The plant know as dang gui (Angelica sinensis tem, stimulates blood circulation balance in females,
3
(Oliv.) Diels), family Umbelliferae, is a very valuable and increases blood flow to the skin. Its estrogen-
1,4
Chinese herb. It has been used for thousands of years like action relieves menopausal syndrome. The ac-
in traditional Chinese medical prescriptions (Zhang tive constituents of dang gui-enriched essential oil
and Cheng, 1989). It is claimed to enrich the blood, (0.4 - 0.7%) are ligustilide and n- butylphthalide,
1
relieve headache and fatigue, and act as a laxative. It phytosteiols, ferulic acid and coumarins (angelol,
1,5
is used for balancing hormone levels in the body, angelicone), buty-lidenephthalide allo-ocimene and
5
including female estrogen hormone, antispasmodic, angelicide, homosenkyunolide H, homosukyunolide
stimulant for blood circulation, and act as a regulator I, neoligustilide, 6-methoxycoumarin, hypoxanthine-
6
9-beta D-ribofuranoside. The purpose of this study
*Medicinal Plant Reserch Institute, Department of Medical Sciences, was to develop a propagation system for A. sinensis
Ministry of Public Health. from rhizomal buds.

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