Page 148 - journal-6-2-S-Full

Journal of Thai Traditional & Alternative Medicine Vol. 6 No. 2 May-August (Supplement) 2008 ÒÙ˜

PO-45

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¿“≥—™ ‡¥™–¬πµå , Õ‘π∑—™ »—°¥‘Ï¿—°¥’‡®√‘≠ , ∫ÿ…∫√√≥ ÿ¢°“πµå , Õ√ÿ≥æ√ Õ‘∞√—µπå
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π—°»÷°…“·æ∑¬å·ºπ‰∑¬ª√–¬ÿ°µå §≥–·æ∑¬»“ µ√å ¡À“«‘∑¬“≈—¬∏√√¡»“ µ√å
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∫—≥±‘µ»÷°…“ §≥–·æ∑¬»“ µ√å ¡À“«‘∑¬“≈—¬∏√√¡»“ µ√å
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»Ÿπ¬å°“√·æ∑¬å·ºπ‰∑¬ª√–¬ÿ°µå §≥–·æ∑¬»“ µ√å ¡À“«‘∑¬“≈—¬∏√√¡»“ µ√å

À≈—°°“√·≈–‡Àµÿº≈ æ≠“«“π√ À√◊Õ Œ«à“πßÁÕ° (Pseuderatherum platiferum) ‡ªìπæ◊™∑’ËÕ¬Ÿà„π«ß»å Acanthaceae µâπ
°”‡π‘¥¢Õßæ≠“«“π√Õ¬Ÿà∑’˪√–‡∑»‡«’¬¥π“¡ ‚¥¬¡’°“√π” à«π¢Õß„∫‰ª„™â‡ªìπ ¡ÿπ‰æ√‡æ◊ËÕ°“√√—°…“‚√§¡–‡√Áߪե
·≈–¡–‡√Áßµ—∫

«—µ∂ÿª√– ß§å »÷°…“ƒ∑∏‘ϧ«“¡‡ªìπæ‘…µàÕ¡–‡√Áß Û ™π‘¥§◊Õ¡–‡√Áߪե Ú ™π‘¥§◊Õ ™π‘¥‡´≈≈å„À≠à COR-L23 ·≈– ™π‘¥
‡´≈≈å‡≈Á° A549 ·≈–¡–‡√Áßµ—∫ ™π‘¥ HepG2 ·≈–ƒ∑∏‘ϵâ“πÕπÿ¡Ÿ≈Õ‘√–¢Õß “√ °—¥„π√Ÿª·∫∫µà“ß Ê¢Õßæ≠“«“π√

«‘∏’¥”‡π‘π°“√ °“√ °—¥¡’∑—ÈßÀ¡¥ ÒÒ “√ °—¥§◊Õ °“√ °—¥‚¥¬„™â„∫ ¥·≈–·Àâß ¥â«¬«‘∏’°“√µâ¡πÈ” À¡—°¥â«¬‡Õ∑“πÕ≈
°—¥¥â«¬°“√„™â Soxhlet apparatus ‚¥¬„™â‡Œ°‡´π §≈Õ‚√øÕ√å¡ ·≈–‡¡∑“πÕ≈ à«π„∫ ¥®–∂Ÿ°π”¡“ªíòπ§—ÈππÈ” °“√
»÷°…“ƒ∑∏‘ϧ«“¡‡ªìπæ‘…µàÕ‡´≈≈å¡–‡√Áߪե·≈–µ—∫¥â«¬«‘∏’ SRB assay ·≈–ƒ∑∏‘ϵâ“πÕπÿ¡Ÿ≈Õ‘ √–¥â«¬«‘∏’ DPPH assay

·≈–À“ª√‘¡“≥ “√°≈ÿà¡øïπÕ≈‘° (total phenolic content) ¥â«¬ Folin-Ciocalteuûs reagent
º≈°“√»÷°…“ æ∫«à“ „∫æ≠“«“π√·Àâß °—¥¥â«¬°“√µâ¡πÈ”„Àâª√‘¡“≥ Ÿß ÿ¥ (Úˆ.ÙÚ%) à«π°“√»÷°…“ƒ∑∏‘ϧ«“¡‡ªìπ

æ‘…µàÕ‡´≈≈å¡–‡√Áß æ∫«à“ “√ °—¥„∫·Àâߥ⫬ ‡Õ∑“πÕ≈ ˘ı ‡ªÕ√凴Áπµå ÕÕ°ƒ∑∏‘ϵâ“π¡–‡√Áߪե™π‘¥ A549 ·≈– COR-
L23 ¥’∑’Ë ÿ¥ (IC = Ú˜.ÒÚ ·≈– Û˘.ÚÚ ‰¡‚§√°√—¡/¡‘≈≈‘≈‘µ√ µ“¡≈”¥—∫) ¡’ƒ∑∏‘ÏπâÕ¬°—∫¡–‡√Áßµ—∫ (IC = ˆı.ˆÛ
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‰¡‚§√°√—¡/¡‘≈≈‘≈‘µ√) à«πƒ∑∏‘ϵâ“πÕπÿ¡Ÿ≈Õ‘ √–æ∫«à“ “√ °—¥„∫æ≠“«“√π√·Àâßµâ¡πÈ”¡’ƒ∑∏‘ϵâ“πÕπÿ¡Ÿ≈Õ‘ √–¥’∑’Ë ÿ¥
√Õß≈ß¡“§◊Õ “√ °—¥„∫æ≠“«“π√ ¥¥â«¬‡Õ∑“πÕ≈ (EC = ı.˘ı ·≈– ˘.ÒÙ ‰¡‚§√°√—¡/¡‘≈≈‘≈‘µ√ µ“¡≈”¥—∫) ‡¡◊ËÕ
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»÷°…“ª√‘¡“≥ “√°≈ÿà¡øïπÕ≈‘° æ∫«à“ “√ °—¥„∫æ≠“«“π√ ·Àâß °—¥¥â«¬ Soxhlet apparatus ¥â«¬‡¡∑“πÕ≈ ¡’
ª√‘¡“≥ “√°≈ÿà¡øïπÕ≈‘°¡“°∑’Ë ÿ¥ √Õß≈ß¡“§◊Õ “√ °—¥„∫æ≠“«“π√ ¥À¡—°¥â«¬‡Õ∑“πÕ≈ ·≈– “√ °—¥„∫ ¥∑’˵â¡πÈ”
(GAE = ÒÛ˜.Ò˜, ÒÚ˜.ı¯ ·≈– ÒÚˆ.˜ı ¡‘≈≈‘°√—¡/°√—¡ µ“¡≈”¥—∫)

¢âÕ √ÿª “√ °—¥„∫æ≠“«“π√ ¥ ¡’ƒ∑∏‘Ï¥’µàÕ ÿ¢¿“æ‚¥¬°“√À¡—°‡Õ∑“πÕ≈·≈–µâ¡πÈ” ∑—Èßπ’È ¡’ƒ∑∏‘ϵâ“πÕπÿ¡Ÿ≈Õ‘ √–∑’Ë
Ÿß ·≈–¡’ “√°≈ÿà¡øïπÕ≈‘° Ÿß à«π “√ °—¥®“°„∫·ÀâßÀ¡—°¥â«¬‡Õ∑“πÕ≈®–¡’º≈¥’µàÕ¡–‡√Áߪե∑—Èß Õß™π‘¥ ·≈–¡’

ƒ∑∏‘χ©æ“–‡®“–®ßµàÕ¡–‡√Áߪե ‡æ√“–¡’ƒ∑∏‘ÏπâÕ¬µàÕ¡–‡√Áßµ—∫º≈°“√∑¥≈Õ߇ªìπ°“√¬◊π¬—π°“√„™âæ≠“«“π√ „π°“√
√—°…“¡–‡√Áß·≈–„™â‡ªìπª√–‚¬™πåµàÕ ÿ¢¿“æ