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OR-24

Ÿ¨ °Š­µ¦­„´—Á‡°¦r‡¼¤·œ (curcumin) ˜n°¡¥µ›·­£µ¡ °Š®œ¼Â±¤­Á˜°¦rš¸É˜·—ÁºÊ°¡¥µ›·ÄÅ¤o˜´

(Opisthorchis viverrini)Ÿnµœ„µ¦Â­—Š°°„ °Š¥¸œÁ„¸É¥ª o°Š„´„µ¦˜µ¥ (apoptosis-related genes)

›·—µ¦´˜œr »¤µ«, ž¦µ–¸ «¦¸¦µ, »«¦µ „oª­¤»š¦, Á˜º°œÄ‹ «¦¸­ªnµŠªŠ«r, Å¡¼¨¥r ­·š›·™µª¦
£µ‡ª·µž¦­·˜ª·¥µÂ¨³ «¼œ¥rª·‹´¥¡¥µ›·ÄÅ¤o˜´Â¨³¤³Á¦ÈŠšn°œÊε—¸ ‡–³Â¡š¥«µ­˜¦r ¤®µª·š¥µ¨´¥ °œÂ„nœ

®¨´„„µ¦Â¨³Á®˜»Ÿ¨ „µ¦˜·—ÁºÊ°¡¥µ›·ÄÅ¤o˜´ (Opisthorchis viverrini) Á¦ºÊ°¦´ŠÁž}œž{‹‹´¥Á­¸É¥Š šÎµÄ®oÁ„·—

懤³Á¦ÈŠšn°œÊε—¸ ™¹ŠÂ¤o‹³¤¸¥µ¡¦µŽ·‡ªµœÁš¨š¸É¤¸ž¦³­·š›·£µ¡Äœ„µ¦¦´„¬µ°»´˜·„µ¦–r„µ¦Á„·—懡¥µ›·
ĝŤo˜´Â¨³¤³Á¦ÈŠšn°œÊε—¸„Ȥ·Å—o¨—œo°¥¨Š °´œÁœºÉ°Š¤µ‹µ„¡§˜·„¦¦¤„µ¦¦·Ã£‡ž¨µ—·Ç­»„ǚεĮo¤¸„µ¦

˜·—ÁºÊ°ŽÊε—´Šœ´Êœ „µ¦¨—¡¥µ›·­£µ¡š¸ÉÁ„·— ¹Êœ‹µ„„µ¦˜·—ÁºÊ°¡¥µ›·ÄÅ¤o˜´‹¹Š¤¸‡ªµ¤­Îµ‡´˜n°„µ¦¨—ž{‹‹´¥

Á­¸É¥Š °Š„µ¦Á„·—¤³Á¦ÈŠšn°œÊε—¸

ª´˜™»ž¦³­Š‡r Á¡ºÉ°«¹„¬µŸ¨ °Š­µ¦­„´—Á‡°¦r‡¼¤·œ‹µ„ ¤·Êœš¸É¤¸„µ¦Äo¡¦n®¨µ¥Â¨³¤¸­¦¦¡‡»–¨—„µ¦°´„Á­
¨³°œ»¤¼¨°·­¦³ Ĝ„µ¦¨—¡¥µ›·­£µ¡ °Š®œ¼Â±¤­Á˜°¦rš¸É˜·—ÁºÊ°—oª¥¡¥µ›·ÄÅ¤o˜´Ÿnµœ„µ¦Â­—Š°°„ °Š

¥¸œš¸ÉÁ„¸É¥ª o°Š„´„µ¦˜µ¥ (apoptosis-related genes)

ª´­—»Â¨³ª·›¸„µ¦ «¹„¬µ¡¥µ›·­£µ¡Ã—¥„µ¦¥o°¤­¸ hematoxylin and eosin Áš‡œ·‡ reverse transcription PCR

(RT-PCR) ¨³„µ¦¥o°¤ TUNEL ץnŠ®œ¼Áž}œÓ„¨»n¤‡º°®œ¼Â±¤­Á˜°¦rš¸É˜·—ÁºÊ°—oª¥¡¥µ›·ÄÅ¤o˜´(OV)
¨³®œ¼˜·—ÁºÊ°š¸ÉÄ®oÁ‡°¦r‡¼¤·œ (OV+Cur) Á„ȝ˜´ª°¥nµŠ·ÊœÁœºÊ°˜´ °Š®œ¼š¸ÉÁª¨µ˜nµŠ Ç „´œ (Ø, ÒÕ, ÓÒ, ÔÑ,

×Ѩ³ ÚѪ´œ®¨´Š„µ¦˜·—ÁºÊ°) ×¥š´ÊŠ­°Š„¨»n¤¤¸®œ¼„¨»n¤‡ª‡»¤ž„˜· (N) ¨³Å—o¦´Á‡°¦r‡¼¤·œ (N+Cur)

Ÿ¨„µ¦«¹„¬µ ¨´„¬–³šµŠ„µ¥ª·£µ‡ °Š˜´¡ªnµšn°œÊε—¸ °Š®œ¼Â±¤­Á˜°¦rš¸É˜·—ÁºÊ°¡¥µ›·š´ÊŠ­°Š„¨»n¤¤¸„µ¦
®œµ˜´ª ¹Êœ˜µ¤¦³¥³Áª¨µ„µ¦˜·—ÁºÊ°­nªœ„µ¦«¹„¬µ¡¥µ›·­£µ¡ °Š¦·Áª–¦° Ç šn°œÊε—¸ °Š˜´Ã—¥„µ¦

¥o°¤­¸ ¡ªnµ„µ¦Á oµ¤µ °ŠÁŽ¨¨r°´„Á­¦° Ç šn°œÊε—¸¤¸‡ªµ¤­´¤¡´œ›r„´¦³—´„µ¦Â­—Š°°„ °Š¥¸œÂ¨³

¡ªnµÁŽ¨¨r°´„Á­¤¸ÂœªÃœo¤¨—¨ŠÄœ„¨»n¤š¸Éŗo¦´Á‡°¦r‡¼¤·œÂ˜nŤn¤¸œ´¥­Îµ‡´Á¤ºÉ°˜¦ª‹ª´—„µ¦Â­—Š°°„ °Š
¥¸œÄœ¦³—´ mRNA °Š¥¸œ Bcl-2 associated protein X (BAX), tumor suppressor gene (p53), caspase 9,

smad1 ¨³ smad4 Ž¹ÉŠÁž}œ¥¸œš¸ÉÁ„¸É¥ª o°Š„´„µ¦Á„·— apoptotic ¨³ serine/threonine protein kinase (PKB)

mouse double minute 2 (MDM2) ¨³ c-ski (oncogene) Ž¹ÉŠÁž}œ anti-apoptotic genes ×¥°µ«´¥Áš‡œ·‡ RT-

PCR ¡ªnµ „µ¦Â­—Š°°„ °Š¥¸œÄœš´ÊŠ­°Š„¨»n¤¤¸¦¼žÂš¸É‡¨oµ¥„´œ ×¥„µ¦Â­—Š°°„ °Š apoptosis-related
genes ‹³Á¦·É¤­¼Š ¹ÊœÄœª´œš¸É ب³­¼Šš¸É­»—ž¦³¤µ–ª´œš¸É ÔÑ ®¨´Š˜·—ÁºÊ° ¨³‹³¨—¨Š®¨´Š‹µ„ª´œš¸É ×Ñ °¥nµŠ¤¸

œ´¥­Îµ‡´šµŠ­™·˜· (p<0.05) Á¤ºÉ°Áž¦¸¥Áš¸¥®œ¼Äœ„¨»n¤ N+Cur ¨³ N ¡ªnµ®œ¼Äœ„¨»n¤ OV+Cur ¤¸„µ¦

­—Š°°„ °Š apoptotic genes ¨³ anti-apoptosis ¤¸ÂœªÃœo¤¨—¨ŠÁ¤ºÉ°Áž¦¸¥Áš¸¥„´„¨»n¤ OV °¥nµŠ
¤¸œ´¥­Îµ‡´šµŠ­™·˜· (p<0.05) ‹µ„„µ¦¥o°¤ TUNEL assay ¡ªnµ¤¸„µ¦˜µ¥ °ŠÁŽ¨¨rš¸Éœ·ªÁ‡¨¸¥­ °ŠÁŽ¨¨r

Á¥ºÉ°» °ŠŸœ´Ššn°œÊε—¸ ™»ŠœÊε—¸ ¨³ÁŽ¨¨r°´„Á­ Ĝ®œ¼š´ÊŠ­°Š„¨»n¤

o°­¦»ž ‹µ„Ÿ¨„µ¦«¹„¬µ‡¦´ÊŠœ¸Ê‡µ—ªnµÁ‡°¦r‡¼¤·œœnµ‹³¤¸Ÿ¨¨—ÁŽ¨¨r°´„Á­ ¨—°œ»¤¼¨°·­¦³­nŠŸ¨Ä®o¨—¡¥µ›·

­£µ¡š¸ÉÁ„·—‹µ„„µ¦˜·—ÁºÊ°¡¥µ›·ÄÅ¤o˜´Ÿnµœ„µ¦Â­—Š°°„ °Š¥¸œš¸ÉÁ„¸É¥ª o°Š„´„µ¦Á„·— apoptosis Ž¹ÉŠ­nŠŸ¨
˜n°„µ¦¨—ž{‹‹´¥Á­¸É¥ŠÄœ„µ¦¡´•œµ„µ¦Á„·—懚µŠ¦³œÊε—¸ ¦ª¤š´ÊŠ¤³Á¦ÈŠšn°œÊε—¸