Page 142 - Acrobat J Trad-21-3-2566

J Thai Trad Alt Med Vol. 21 No. 3 Sep-Dec 2023 625

as following: 0-5 min, 5%A : 95%B; 5-20 min, 8. Statistical analysis
5%A : 95%B – 20%A : 80%B; 20-30 min, 20%A : The experiments were conducted in trip-

80%B – 70%A : 30%B; 30-35 min, 70%A : 30%B; licate and the results were reported as mean ±
35-40 min 5%A : 95%B. The flow rate was set at standard deviation (SD). The IC 50 values were
1.0 mL/min, and the analysis was conducted at calculated by using a regression analysis. The

room temperature. Mitragynine was detected differences between groups were statistically
at a wavelength of 245 nm. Standard solutions analyzed using one-way ANOVA with post hoc
were prepared with concentrations ranging analysis. GraphPad Prism software (CA, USA)

from 10 to 250 µg/mL. To ensure the quality was utilized for statistical analysis.
of the analysis, quality control (QC) samples
were prepared by spiking standard solution Results

into sample solution at concentrations 15, 75 The extraction yields of the Kratom ex-
and 150 µg/mL. The extracts were prepared by tracts are shown in Table 1. The 70% ethanol

diluting with methanol and filtered through a extract (KE70) exhibited the highest extraction
0.45 µm membrane filter prior to injection into yield, whereas the 95% ethanol extract (KE95)
the HPLC system. demonstrated the lowest.

Table 1 Extraction yield of Kratom leaves extracts

Extraction solvent Extraction method Code Extraction Yield (%)

95% Ethanol maceration KE95 18.8
70% Ethanol maceration KE70 36.2
50% Ethanol maceration KE50 34.5
Water decoction KDec 31.5

1. In vitro inhibitory effect on alpha- in Figure 1. All extracts at a concentration of

glucosidase and alpha-amylase 1,000 µg/mL demonstrated activity of less

The anti-diabetes activity of the Kra- than 50%, indicating that they were ineffec-
tom extracts were evaluated using alpha- tive in inhibiting the alpha-amylase enzyme.

glucosidase and alpha-amylase enzymes. However, for alpha-glucosidase, KE70 and the
Prior to determining IC 50, the % inhibition of 50%etahnolic extract (KE50) exhibited inhibi-
the Kratom extracts was screened, as shown tory activity greater than 50%, and were thus