Page 82 - journal-8-2-S-Full

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PP-33

The study of cucurbitacins extracted from Trichosanthes cucumerina L. on

breast cancer cell lines.

Suwit Duangmano, Pornvaree Lamchiagdhase, Vichanan Yamkamon, Tippawan Wongchan, Rawiwan
Phohom, Pimpicha Patmasiriwat

Department of Clinical Microscopy, Faculty of Medical Technology, Mahidol University. Salaya, Phuttamonthon,
Nakhonpathom 73170

Rationale: Trichosanthes cucumerina L., named in Thai çBuap-Khomé belongs to Cucurbitaceae family.
This plant has been used as anthehelmintic and antipyretic in Thai alternative medicine. The extraction from
dry fruit of T. cucumerina contained cucurbitacins which had broad range of potent biological activities such as
cytotoxicity, antitumor, anti-inflammatory and antimicrobial activities. Our previous study revealed that both
crude spray-dried extract and partially purified extracts, the cucurbitacin B and dihydrocucurbitacin B, from

this herb could reduce cell proliferation and induced apoptosis of breast cancer and leukemic cell lines. In this
study, we further investigate bioactivities of other highly purified forms of cucurbitacins (-G, -H, -I) compares to
cucurbitacin B.

Objectives: To investigate the effect of cucurbitacin B, cucurbitacin G, cucurbitacin H and cucurbitacin
I, extracted from T. cucumerina, on the cell growth inhibition of breast cancer cell lines SKBR3 compared with
normal breast cell line HBL-100 by the MTT assay and to determine cell cycle alteration of treated cells.
Methodology: Breast Cell lines were grown in DMEM/F 12 medium supplemented with 10 % FBS. Cells
o
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were plated in 96-well plated at 10 cells/well and incubated for 24 hours at 37 C, 5%CO . Then, cells were
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treated with various concentration of cucurbitacins, which kindly provided by Prof. Dr. Apichart Suksamrarn,
Faculty of Science, Ramkhamhaeng University. After exposure to the extracts for 24 hours, the MTT assay and
cell cycle analysis were performed. In cell cycle analysis, the cells were collected and measured by FACSCalibur

using CellQuest software.
Results: Cucurbitacin B and cucurbitacin I could inhibit the cell viability of SKBR3 with IC value of 3.29
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and 26.45 μg/ml, respectively. The cell growth inhibition effect of cucurbitacin B on breast cancer cell is higher
than the effect of cucurbitacin I, whereas cucurbitacin I has higher cytotoxicity effect on normal breast cell. The
IC value for HBL-100 were 32.77 and <20 μg/ml, respectively. Interestingly, cell cycle analysis of SKBR3 and
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HBL-100 after treated with cucurbitacin B for 24 hours, demonstrated the G /M phase cell cycle arrest.
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Conclusion: Cucurbitacin B and cucurbitacin I, extracted from T. cucumerina, affected cell growth inhi-
bition of breast cancer cells. One mechanism of cucurbitacin B on breast cells is G /M phase cell cycle arrest.
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However, other mechanisms of cucurbitacin B and cucurbitacin I on breast cancer cell are interesting for
further study in order to be therapeutic use in breast cancer in the future.