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P. 56

Journal of Thai Traditional & Alternative Medicine Vol. 7 No. 2-3 May-December 2009 Òı˘

“√‡§¡’·≈– “√¡“μ√∞“π ¿“«–°“√∑¥ Õ∫

Ò. °√¥‰πμ√‘°‡¢â¡¢âπ (ˆı%) ™π‘¥‡°√¥«‘‡§√“–Àå¢Õß Element Asenic
∫√‘…—∑ Merck. Lamp Electrodeless discharge

Ú. “√≈–≈“¬æ—≈≈“‡¥’¬¡ ‰π‡μ√μ (Pd Ò. g/l) AA lamp (EDL) current 380 mA
Grade (Fluka). Slit 0.7 nm
Û. “√≈–≈“¬¡“μ√∞“π “√ÀπŸ AA Grade §«“¡‡¢â¡¢âπ Wavelength 193.7 nm

Ò à«π„π≈â“π à«π (Perkin Elmer). Injection volume 20 μm
Ù. πÈ”∑’Ë„™â‡μ√’¬¡ “√≈–≈“¬¡“μ√∞“π ‡μ√’¬¡ “√≈–≈“¬ Matrix modifier Palladium 5 μg
μ—«Õ¬à“ß ·≈–≈â“ß‡§√◊ËÕß·°â«À√◊Õ¢«¥æ≈“ μ‘°‡ªìππÈ”Õ—≈μ√â“ Pyrolysis temperature 1,200 ˚´.

‡æ’¬« ∑’Ë¡’§«“¡μâ“π∑“πÕ¬à“ßπâÕ¬ Òˆ ‡¡°–‚ÕÀå¡ (Mohm) Atomization temperature 2,200 ˚´.
À√◊ÕπÈ”°≈—Ëπ Û §√—Èß. Atomization time 3 sec
- ∑¥ Õ∫§«“¡‰«¢Õß‡§√◊ËÕß¡◊Õ„Àâ‰¥â —≠≠“≥ Ÿß ÿ¥ ∑’Ë
°“√‡μ√’¬¡°√¥‰πμ√‘° .Ú% v/v
¡¡“μ√ ·≈–‰¥â§à“ absorbance Õ¬Ÿà„π™à«ß ± √âÕ¬≈– Ú
π”°√¥‰πμ√‘°‡¢â¡¢âπ .Ú ¡‘≈≈‘≈‘μ√ ¡“‡®◊Õ®“ß·≈–ª√—∫ ¢Õß§à“∑’ËºŸâº≈‘μ‡§√◊ËÕß¡◊Õ°”Àπ¥‰«â ‚¥¬π” “√≈–≈“¬¡“μ√∞“π

ª√‘¡“μ√¥â«¬πÈ”®π§√∫ Ò ¡‘≈≈‘≈‘μ√. “√ÀπŸ§«“¡‡¢â¡¢âπ ı à«π„πæ—π≈â“π à«π¡“«—¥§à“ absor-
bance §«√‰¥â§à“ integrated peak area ‡∑à“°—∫ .ÒÒ
°“√‡μ√’¬¡ “√≈–≈“¬¡“μ√∞“π
À√◊Õ§”π«≥§à“ characteristic mass ®–‰¥â‡∑à“°—∫ Ù.

‡μ√’¬¡ “√≈–≈“¬¡“μ√∞“π “√ÀπŸ§«“¡‡¢â¡¢âπ ı à«π æ‘‚§°√—¡ μàÕ integrated peak area .ÙÙ.
„πæ—π≈â“π à«π (æ’æ’∫’) ‚¥¬π” “√≈–≈“¬¡“μ√∞“π “√ÀπŸ - ∑¥ Õ∫°“√ªπ‡ªóôÕπ‚¥¬„™âπÈ” (reagent blank)
§«“¡‡¢â¡¢âπ Ò à«π„π≈â“π à«π (æ’æ’‡ÕÁ¡) ¡“‡®◊Õ®“ß·≈– ·∑π “√≈–≈“¬μ—«Õ¬à“ß ·≈–«—¥§à“Õ”π“®°“√¥Ÿ¥´÷¡ ´÷Ëß‰¡à

ª√—∫ª√‘¡“μ√¥â«¬°√¥‰πμ√‘° .Ú% v/v. §«√¡’§à“ integrated peak area ¡“°°«à“ .Ú. ∂â“¬—ß§ß
¡“°°«à“„Àâμ√«® Õ∫°“√ align tip À√◊ÕÕÿª°√≥åÕ◊Ëπ Ê ∑’ËÕ“®
°“√‡μ√’¬¡ “√≈–≈“¬μ—«Õ¬à“ß
∑”„Àâ‡°‘¥°“√ªπ‡ªóôÕπ‰¥â ·≈â«∑¥ Õ∫„À¡à. ∂â“§à“ absorbance
π”μ—«Õ¬à“ß™“ ¡ÿπ‰æ√ Ò ´Õß (πÈ”Àπ—°ª√–¡“≥ Ú °√—¡) ¬—ß§ß¡“°Õ¬Ÿà · ¥ß«à“¬—ß§ß¡’°“√ªπ‡ªóôÕπ „Àâ‡μ√’¬¡μ—«Õ¬à“ß
·™à„ππÈ”μâ¡‡¥◊Õ¥„À¡à Ê Ú ¡‘≈≈‘≈‘μ√ μ—Èß∑‘Èß‰«â Ú π“∑’ ‡æ◊ËÕ∑¥ Õ∫„À¡à∑—ÈßÀ¡¥.

Õÿ≥À¿Ÿ¡‘¢ÕßπÈ”™“™ß®–≈¥≈ß‡À≈◊Õª√–¡“≥ ı˚´ °√Õß¥â«¬ - √â“ß°√“ø¡“μ√∞“π®“° “√≈–≈“¬¡“μ√∞“π “√ÀπŸ
°√–¥“…°√Õß. §«“¡‡¢â¡¢âπ ı à«π„πæ—π≈â“π à«π √â“ß°√“ø¡“μ√∞“π
· ¥ß§«“¡ —¡æ—π∏å√–À«à“ß§à“ absorbance ·≈–§«“¡‡¢â¡
°“√‡μ√’¬¡ “√≈–≈“¬‰√â “√ (reagent blank)
¢âπ¢Õß “√ÀπŸ ı, Ò, Ú, Ûı ·≈– ı à«π„πæ—π≈â“π à«π
π”πÈ”μâ¡‡¥◊Õ¥„À¡à Ê Ú ¡‘≈≈‘≈‘μ√ μ—Èß∑‘Èß‰«â Ú π“∑’ ‚¥¬μ—Èß√–∫∫°“√∑”ß“π¢Õß‡§√◊ËÕß„Àâ‡®◊Õ®“ß “√≈–≈“¬

„ÀâÕÿ≥À¿Ÿ¡‘≈¥≈ß‡À≈◊Õª√–¡“≥ ı ˚´ °√Õß¥â«¬°√–¥“…°√Õß. ¡“μ√∞“πÕ—μ‚π¡—μ‘ ·≈–°”Àπ¥„Àâ§«“¡‡ªìπ‡ âπμ√ß¢Õß°√“ø
¡“μ√∞“πÕ¬à“ßπâÕ¬‡∑à“°—∫ .˘˘ı.
«‘∏’«‘‡§√“–Àå
- «‘‡§√“–ÀåÀ“ª√‘¡“≥ “√ÀπŸ„π “√≈–≈“¬‰√â “√·≈–

°àÕπ«‘‡§√“–ÀåÀ“ª√‘¡“≥ “√ÀπŸ„π “√≈–≈“¬μ—«Õ¬à“ß μâÕß “√≈–≈“¬μ—«Õ¬à“ß ‚¥¬©’¥ “√≈–≈“¬‰√â “√ ·≈– “√≈–≈“¬
μ√«® Õ∫°“√ align tip μ“¡«‘∏’°“√∑’Ë√–∫ÿ„π§Ÿà¡◊Õ «‘∏’„™â μ—«Õ¬à“ß Õ¬à“ß≈– Ú ‰¡‚§√≈‘μ√ ·≈â«π”‰ª«—¥§à“ absorbance
‡§√◊ËÕß∑¥ Õ∫§«“¡‰« (instrument optimization) ·≈– μ“¡ ¿“«–°“√∑¥ Õ∫∑’Ë°”Àπ¥‰«â ´÷Ëß‡§√◊ËÕß®–§”π«≥ °≈—∫

∑¥ Õ∫°“√ªπ‡ªóôÕπ. ¡“‡ªìπª√‘¡“≥ “√ÀπŸ (æ’æ’∫’) ‚¥¬‡∑’¬∫°—∫°√“ø¡“μ√∞“π.

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