Page 166 - journal-6-2-S-Full

Journal of Thai Traditional & Alternative Medicine Vol. 6 No. 2 May-August (Supplement) 2008 Òˆı

°“√»÷°…“§«“¡∂Ÿ°µâÕß·¡àπ¬”¢Õß«‘∏’«‘‡§√“–Àå “√ÕÕ°ƒ∑∏‘Ï Madecassoside ·≈–

Asiaticoside „𬓇¡Á¥∫—«∫° ·≈–‡®≈∫—«∫°

Ò Ò Ú
ÿ«√√≥“ ‡À≈◊Õß™≈∏“√ , ™”π“≠ ¿—µ√æ“π‘™ , ª∞¡ ‚ ¡«ß»å
Ò
¿“§«‘™“‡¿ —™‡§¡’ §≥–‡¿ —™»“ µ√å ®ÿÓ≈ß°√≥å¡À“«‘∑¬“≈—¬
Ú
§≥–‡¿ —™»“ µ√å ¡À“«‘∑¬“≈—¬√—ß ‘µ

À≈—°°“√·≈–‡Àµÿº≈ ª√‘¡“≥ “√ Madecassoside (MS) ·≈– Asiaticoside (AS) ´÷Ë߇ªìπ “√ÕÕ°ƒ∑∏‘Ï„π ¡ÿπ‰æ√∫—«∫°
·ª√º—πµ“¡™π‘¥¢Õßæ—π∏ÿåæ◊™ Õ“¬ÿ ƒ¥Ÿ°“≈ ·≈– ¿“æ·«¥≈âÕ¡„π°“√‡®√‘≠‡µ‘∫‚µ¢Õßæ◊™ ·≈–‡¡◊ËÕπ”∫—«∫°¡“ °—¥
‡µ√’¬¡‡ªì𬓇¡Á¥∫—«∫°·≈–‡®≈∫—«∫° ®÷ß¡—°¡’ªí≠À“„π‡√◊ËÕߧ«“¡ ¡Ë”‡ ¡Õ¢Õߪ√‘¡“≥ “√ ”§—≠„π«—µ∂ÿ¥‘∫∑’Ëπ”¡“„™â

¥—ßπ—Èπ®÷߉¥â»÷°…“À“«‘∏’«‘‡§√“–Àå “√ MS ·≈– AS ‡æ◊ËÕ°“√§«∫§ÿ¡§ÿ≥¿“欓‡¡Á¥∫—«∫° ·≈–‡®≈∫—«∫°

«—µ∂ÿª√– ß§å ‡æ◊ËÕ»÷°…“§«“¡∂Ÿ°µâÕß·¡à𬔠(validation) ¢Õß«‘∏’«‘‡§√“–Àå “√ÕÕ°ƒ∑∏‘Ï MS ·≈– AS „𬓇¡Á¥∫—«∫°
·≈–‡®≈∫—«∫°¥â«¬«‘∏’ TLC-Densitometry ∑’ˉ¥âæ—≤π“¢÷Èπ

«‘∏’¥”‡π‘π°“√ Ò) »÷°…“§«“¡∂Ÿ°µâÕß·¡àπ¬”¢Õß«‘∏’«‘‡§√“–Àå “√ÕÕ°ƒ∑∏‘Ï MS ·≈– AS „𬓇¡Á¥∫—«∫°·≈–‡®≈∫—«∫°
¥â«¬«‘∏’ TLC-Densitometry ∑’ˉ¥âæ—≤π“¢÷È𠵓¡¢âÕ°”Àπ¥ ICH Ú) «‘‡§√“–ÀåÀ“ª√‘¡“≥ “√ MS ·≈– AS „𬓇¡Á¥
∫—«∫°·≈–‡®≈∫—«∫°∑’˺≈‘µ¢÷Èπ

º≈°“√»÷°…“ Ò) º≈°“√»÷°…“§«“¡∂Ÿ°µâÕß·¡àπ¬”¢Õß«‘∏’«‘‡§√“–Àå “√ÕÕ°ƒ∑∏‘Ï MS ·≈– AS „𬓇¡Á¥∫—«∫°·≈–‡®

≈∫—«∫°¥â«¬«‘∏’ TLC-Densitometry ∑’ˉ¥âæ—≤π“¢÷Èπæ∫«à“«‘∏’¥—ß°≈à“«‡¢â“‡°≥±å¢âÕ°”Àπ¥ ICH ‚¥¬¡’√“¬≈–‡Õ’¬¥¥—ßπ’È Ò.Ò)
¡’ specificity ¥’ ‰¡à¡’ “√„¥ Ê √∫°«π, Ò.Ú) ¡’ percentage recovery ¢Õß “√ MS = ˘˜.ÙÛ ± .ˆı ·≈– ˘˘.˜
± .ÙÚ ·≈–¢Õß “√ AS= ˘˜.˜ı ± Ò.Ò˘ ·≈– Ò.ÛÙ ± Ò.Ú˘ µ“¡≈”¥—∫, Ò.Û) ¡’ repeatability ¥’ ‚¥¬¡’§à“
2
%RSD ¢Õß “√ MS= .˜ı ·≈– Ò.¯Ú ¢Õß “√ AS= .¯Ù ·≈– Ò.¯Ù µ“¡≈”¥—∫, Ò.Ù) ¡’§à“ linearity R > .˘˘,
Ò.ı) ¡’ range (¡§°.) ¢Õß “√ MS Õ¬Ÿà„π™à«ß .¯ˆ-Ù.Ú˘ ·≈– .Ò˜Ú-Ú.ˆ ·≈–¢Õß “√ AS Õ¬Ÿà„π™à«ß .˜¯-

Û.¯¯ ·≈– .Òıˆ-Ò.¯ˆÚ µ“¡≈”¥—∫, Ò.ˆ) ¡’ lower limit of detection (LLOD)(¡§°.) ¢Õß “√ MS = .ÒÛˆ ·≈–
.¯ı ·≈–¢Õß “√ AS= .˘Û ·≈– .ıÒ µ“¡≈”¥—∫, Ò.˜) ¡’§à“ lower limit of quantitation (LLOQ)(¡§°.)
¢Õß “√ MS .ÙÒÒ ·≈– .Ò˜Ú ·≈–¢Õß “√ AS = .Ú¯Ú ·≈– .Òıˆ µ“¡≈”¥—∫, Ú) º≈°“√«‘‡§√“–À嬓‡¡Á¥

∫—«∫° ·≈–‡®≈∫—«∫°æ∫«à“¡’ “√ MS ‡©≈’ˬ ˘˜.Ù¯ ± Ù.ÛÒ %LA ·≈– ˘Ù.¯Ú ± Ù.Û˘ %LA µ“¡≈”¥—∫ ·≈– “√
AS ‡©≈’ˬ ˘˜.ÙÛ ± Û.Úı %LA ·≈– ˘ˆ.˘Ò ± Ù.˜˘ %LA µ“¡≈”¥—∫

¢âÕ √ÿª °“√«‘‡§√“–Àå “√ÕÕ°ƒ∑∏‘Ï MS ·≈– AS„𬓇¡Á¥∫—«∫°·≈–‡®≈∫—«∫°¥â«¬«‘∏’ TLC- Densitometry ∑’ˉ¥âæ—≤π“
¢÷Èππ’È ¡’§«“¡∂Ÿ°µâÕß·¡à𬔠Ÿß ‡™◊ËÕ∂◊Õ‰¥â «‘∏’π’ȇªìπ«‘∏’∑’Ëßà“¬ –¥«° √«¥‡√Á« ·≈–ª√–À¬—¥ ¡’§«“¡‡À¡“– ¡„π°“√„™â
§«∫§ÿ¡§ÿ≥¿“欓‡¡Á¥∫—«∫°·≈–‡®≈∫—«∫°