Page 137 - journal-6-2-S-Full

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PO-34

Anti P. acne activity of cream containing aromatic volatile oil from

Thai medicinal plants entrapped in niosomes for acne treatment

1,2 2 2 1,2
Aranya Manosroi , Thongchai Sritapunya , Pisit Jainonthee , Jiradej Manosroi *
1
Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand
2
Natural Products Research and Development Center (NPRDC), Institute for Science and Technology Research and Development,
Chiang Mai University, Thailand 50200.

Rational: Propionibacterium acnes is a bacteria typically found on human skin and cause acnes. Many aromatic
volatile oils from Thai medicinal plants have been reported to have antibacterial activity. This study has developed

a cream formulation containing aromatic volatile oils from Thai medicinal plants for acne treatment.

Objective: The aim of this study was to investigate antibacterial activity against P. acnes of cream containing
mixed aromatic volatile oil extracted from Thai medicinal plants and entrapped in niosomes.

Methods: Niosomes composed of Tween61/cholesterol at 1:1 molar ratio entrapped with the mixture of aromatic
volatile oils from Thai medicinal plants (kaffir lime leaf oil, hairy basil oil, holy basil oil, sweet basil oil and lemon
grass oil) were prepared by chloroform film method with sonication and incorporated in the cream base. The

antibacterial activity against P. acnes was investigated by agar disc diffusion method. Briefly, the agar media
swabbed with P. acnes was treated with 0.1 g of the sample in a disc and incubated under anaerobe condition at
o
37 C for 48 hours. The antibacterial activity of the sample was investigated as the diameters of the clear zone in
comparison with the standard erythromycin.

Results: The results revealed that cream containing niosomes entrapped with the mixture of the aromatic volatile

oil and 2% salicylic acid and cream containing 2% salicylic acid only exhibited antibacterial activity against P.
acnes with the clear zone diameter of 21.33 ± 0.5 and 17.66 ± 0.5 mm., respectively. The standard erythromycin
gave the clear zone diameter of 26.33 ± 0.5 mm. This study has demonstrated that niosomes composed of
Tween61/ cholesterol at 1:1 molar ratio entrapped with the mixture of aromatic volatile oil extracted from Thai
medicinal plants enhanced antibacterial activity of P. acnes of the cream formulation containing 2% salicylic acid.

Conclusion: The highest anti P. acnes activity was found in cream containing niosomes entrapped with the mixed
aromatic volatile oil and 2% salicylic acid with the clear zone diameters of 21.33 ± 0.5 mm. The incorporation of
mixed oil entrapped in niosomes in cream containing 2% salicylic acid gave the synergistic anti P. acne effects of
salicylic acid. The result from this study can be used to develop topical formulations for acne treatment.