Page 133 - journal-6-2-S-Full

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Development of shampoo containing extract from Bai Mee

(Litsea glutinosa (Lour.) C.B. Rob) entrapped in niosomes

1,2 1,2 2 1,2
Aranya Manosroi , Chonlada Komno , Pisit Jainonthee , Jiradej Manosoi
1
Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand
2
Natural Products Research and Development Center (NPRDC), Institute for Science and Technology Research and Development,
Chiang Mai University, Thailand 50200.

Rationale: Bai Mee (Litsea glutinosae (Lour.) C.B. Rob) Family Lauraceae is a Thai medicinal plant which has
mucilage (polysaccharide) as the main component. Mucilage can protect moisture loss from hair and skin. The

application of nanovesicles in the forms of niosomes can increase the efficiency of skin absorption of various
substances. So, an increase in hair adsorption of the extract from Bai Mee entrapped in niosome is anticipated.

Objective: The aim of this study was to characterize the Bai Mee extract and the extract entrapped in niosomes
and developed the shampoo formulation containing Bai Mee extract entrapped in niosomes.

Methods: The leaves of the plant were dried and extracted with water and sonication. Proteins and total reducing
sugar in crude extract was analyzed by dinitrosalicylic acid-spectrophotometry method and Biuret test. The extract

solution was treated on hair for physical appearance investigation in comparison with the untreated hair. Physical
properties of the hair were investigated under optical microscope (1000x). The extract was entrapped in niosomes
by the conventional chloroform film method with sonication and incorporated in the shampoo base. The formula-
o o
tion was investigated for physical stability when kept at 4 C for 24 hours and 30 C for 4 hours for 4 cycles and
tested for skin irritation in rabbits.

Results: The results showed that the percentage yield of the extract from Bai Mee leaves was 17.5% with 10-15%
of protein and more than 90% of reducing sugar. The hair treated with the extract was better in appearance,
smoothness and combing ability than the untreated hair. The maximum loading of the extract in niosomes (Tween
61/cholesterol at 1:1 molar ratio) was 0.05%. The average particle size of the niosomes was 100.4 nm. The
developed shampoo formulation containing 0.05% of the extract and 0.5% of the niosomes entrapped with the
o o
extract was stable when kept at 4 C for 24 hours and 30 C for 4 hours for 4 cycles and gave no skin irritation in
rabbit patch test.

Conclusion: The developed shampoo formulation containing the extract and the niosomes entrapped with the
extract which was stable and no irritation in rabbit skin was selected for further development in hair products.