Page 114 - วารสารการแพทย์แผนไทย ปีที่ 21 ฉบับที่ 2

330 วารสารการแพทย์แผนไทยและการแพทย์ ทางเลือก ปีที่ 21 ฉบับที่ 2 พฤษภาคม-สิงหาคม 2566

sists of 21 herbal plants, 50% (w/w) of which nents of Ya Prasa Pro Yai has been reported
are PraoHom (Kaempferia galanga L.) which to prevent quercetin binding to Mpro main

was reported to show various biological ac- protease and thus has potential as Sar-CoV-2
[14]
tivities such as antimicrobial, antioxidant, inhibitor. YaNang leaves extract also has
amebicidal, analgesic, anti-inflammatory, anti- been reported to inhibited Delta variant of

tuberculosis, anti-dengue, anti-nociceptive, SARS-CoV-2 virus with 95.95% inhibition at
[12]
[15]
anti-angiogenic and anti-cancer. Ya Ha Rak 10 mg/mL. and Koad-Chulalumpa, one of the
(The five roots drug) which has gained recog- herbal components in Chantaleela has been
nition for its anti-pyretic properties which are reported to inhibit Sar-CoV-2 with the EC 50
recorded in the ancient Thai pharmacopeia. below 0.1 mg/mL using the plaque reduction
The formulation is composed of root powder assay. Taking these reports into account
[16]
from 5 plants including Ya-Nang (Tiliacora tri- there seems to be evidence to suggest that
andra (Colebr.) Diels), Konta (Harrisonia perfo- Thai traditional herbal formulations may be

rata (Blanco) Merr.), MaDueiChumphon (Ficus useful for the treatment of Covid-19. [17-18]
racemosa L.), ChingChi (Capparis micracantha The research presented here therefore
DC.) and MaiTaoYayMom (Clerodendrum aimed to investigate the anti-viral properties of

indicum (L.) Kuntze. The Ya Chanthalila for- Ya Prasa Pro Yai, Ya Ha Rak and Ya Chanthalila
mulation is composed of eight kinds of herbal formulation extracts.

plants including Koad-Kamao (Atractylodes
lancea (Thunb) DC.), Koad-So (Angelica da- Methodology
hurica (Fisch.ex Hoffm.) Benth. et Hook. f.),

Koad-Chulalumpa (Artemisia vulgaris Linn.), 1. Materials
Chan-Daeng (Dracaena loureiroi Gagnep.), 1.1 Cell culture
Chan-Khoaw (Tarenna hoaensis Pit.), Pla-Lai- Vero E6 cells, African green monkey kid-

Peark (Eurycoma longifolia Jack.), Ka-Dom ney epithelial cells and Calu-3, lung epithelial
(Gymnopetalum cochinense Kurz H.C.) and cell line (ATCC, NY, USA), were grown in
Bor-ra-pet (Tinospora crispa Miers ex. Hook. Eagle’s minimum essential medium (EMEM)

f.). The herbal plants in the formulation were (Gibco, NY, USA) cell culture medium con-
previously reported to have biological activi- taining 2 mM L-glutamine, 0.1 mM non-

ties including Anti-oxidant, anti-inflammatory, essential amino acid, 1 mM sodium pyruvate
antinociceptive and antipyretic. and 10% fetal bovine serum at 37 ˚C in 5% CO 2
[13]
K. galanga L. one of the major compo- incubator.