Page 97 - วารสารปีที่15ฉบับที่3

363

Abstract
Antibacterial Activity Against Bacillus cereus of Lupinifolin from the Stem
of Derris reticulata Craib.

Kamol Yusook*
Public health programme, Phetchabun Rajabhat university, Phetchabun 67000, Thailand
*Corresponding author: vetgetmoonlight@hotmail.com

Bacillus cereus is the bacterium commonly causes food poisoning in human. The contamination of
Bacillus cereus in food has been found at a high rate. This study aimed to investigate the antibacterial effects
of lupinifolin, a bioactive compound extracted from Derris reticulata against this pathogen. Lupinifolin
showed the inhibitory effect in B.cereus growth both on MHB agar cultured-bacteria and Disc diffusion
method different from ampicillin (p < 0.05). Furthermore, Microdilution method showed MIC and MBC of
the pure compound were 8 and 16 μg/ml, respectively. Under electron microscope, lupinifolin was found to
be capable of destroying cell wall and swelling cell membrane of Bacillus cereus. In conclusion, the present
results suggests that lupinifolin might exert its anti-B.cereus activity by altering the normal functions of cell
wall and cell membrane. Thus, lupinifolin could be a potential compound for the development of antibacterial
drugs in the future.
Key words: lupinifolin, Derris reticulata Craib., Bacillus cereus

∫∑π”
√—°…“„πªí®®ÿ∫—π ®–„™â¬“μâ“π®ÿ≈™’æ ´÷Ë߇ªìπ “√‡§¡’

ªí®®ÿ∫—π°“√ªπ‡ªóôÕπ„πÕ“À“√ ¡’ “‡Àμÿ¡“®“° ∑’Ë —߇§√“–Àå¢÷Èπ·≈–π”‡¢â“®“°μà“ߪ√–‡∑» ∑”„Àâ ‘Èπ
‡™◊ÈÕ‚√§À≈“¬™π‘¥ ‰¥â·°à ·∫§∑’‡√’¬ ‰«√— ·≈– ‡ª≈◊Õß§à“„™â®à“¬„π°“√√—°…“‡ªìπ®”π«π¡“° ®“°
欓∏‘μà“ß Ê ´÷Ë߇ªì𠓇Àμÿ¢Õß°“√‡°‘¥‚√§Õ“À“√ ¢âÕ¡Ÿ≈¢Õß ”π—°ß“π§≥–°√√¡°“√Õ“À“√·≈–¬“ (Õ¬.)

‡ªìπæ‘… (Food poisoning) ®“°√“¬ß“π°“√‡ΩÑ“ æ∫«à“ª√–‡∑»‰∑¬¡’°“√π”‡¢â“·≈–º≈‘쬓ªØ‘™’«π–°«à“
√–«—ß‚√§ ”π—°√–∫“¥«‘∑¬“ °√¡§«∫§ÿ¡‚√§ 20 % ¢Õ߬“∑—ÈßÀ¡¥ À√◊Õª√–¡“≥ 2 À¡◊Ëπ≈â“π∫“∑
°√–∑√«ß “∏“√≥ ÿ¢ μ—Èß·μà 1 ¡°√“§¡ 2559 - 20 μàÕªï °“√§‘¥§â𬓙𑥄À¡à∑’Ë °—¥‰¥â®“°æ◊™ ¡ÿπ‰æ√

¡‘∂ÿπ“¬π 2559 æ∫ºŸâªÉ«¬‚√§Õ“À“√‡ªìπæ‘…®“°°“√ ®÷߇ªìπ«‘∂’∑“ßÀπ÷ËߢÕß°“√æ÷Ëßμπ‡Õß ·≈–≈¥§«“¡
√—∫ª√–∑“πÕ“À“√ªπ‡ªóôÕπ‡™◊ÈÕ·∫§∑’‡√’¬®”π«π ‘Èπ‡ª≈◊Õß∑“߇»√…∞°‘®®“°°“√π”‡¢â“¬“®“°μà“ß
58,168 §π ∑—Ë«ª√–‡∑» §‘¥‡ªìπÕ—μ√“ªÉ«¬ 88.91 μàÕ ª√–‡∑»

· πª√–™“°√ ‡™◊ÈÕ Bacillus cereus ‡ªìπ‡™◊ÈÕ ‚√§Õ“À“√‡ªìπæ‘… ¡’ “‡Àμÿ¡“®“°ªí®®—¬À≈“¬
·∫§∑’‡√’¬™π‘¥Àπ÷Ëß∑’Ëæ∫¡’°“√ªπ‡ªóôÕπÕ¬Ÿà„πÕ“À“√ Õ¬à“ß Õ“®‡°‘¥®“°¡’°“√ªπ‡ªóôÕπ “√æ‘…À√◊Õ‡™◊ÈÕ‚√§

·≈–‡ªì𠓇Àμÿ¢Õß°“√‡°‘¥‚√§Õ“À“√‡ªìπæ‘… ∑”„Àâ „πÕ“À“√ B. cereus ‡ªìπ‡™◊ÈÕ·∫§∑’‡√’¬™π‘¥Àπ÷Ëß∑’ˇªìπ
[1,2]
ºŸâªÉ«¬∑’ˉ¥â√—∫‡™◊ÈÕ¡’Õ“°“√∑âÕ߇ ’¬·≈–Õ“‡®’¬π °“√ “‡Àμÿ¢Õß‚√§π’È à«π„À≠àæ∫‰¥â„πÕ“À“√ª√–‡¿∑