Page 100 - วารสารปีที่15ฉบับที่3

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‡æ“–‡≈’Ȭ߇™◊ÈÕÕÿ≥À¿Ÿ¡‘ 37 Õß»“‡´≈‡´’¬ ‡ªìπ‡«≈“ 24 ≈—°…≥–¢Õ߇´≈≈å·∫§∑’‡√’¬

™—Ë«‚¡ß §à“ MIC §◊Õ§à“§«“¡‡¢â¡¢âπμË” ÿ¥∑’ˉ¡à‡ÀÁπ
º≈°“√»÷°…“
°“√‡®√‘≠‡μ‘∫‚μ¢Õ߇™◊ÈÕ·∫§∑’‡√’¬ À√◊Õ ‰¡à‡°‘¥

§«“¡¢ÿàπ «‘‡§√“–Àå§à“ MBC ¥â«¬°“√π”Õ“À“√‡≈’È¬ß ®“°°“√ °—¥≈”μâπ™–‡Õ¡‡Àπ◊Õ ‡æ◊ËÕÀ“ “√≈Ÿªîπ‘-
‡™◊ÈÕ®“°À≈ÿ¡∑’ˉ¡à‡°‘¥§«“¡¢ÿàπ‰ª‡æ“–‡≈’Ȭ߇™◊ÈÕ∫π øÕ≈‘π ¥â«¬«‘∏’ Soxhlet extraction ‚¥¬„™â‡Œ°‡´π
MHA ·≈â«π”‰ª∫à¡„πμŸâÕ∫‡æ“–‡≈’Ȭ߇™◊ÈÕÕÿ≥À¿Ÿ¡‘ 37 ‡ªìπμ—«∑”≈–≈“¬ ·≈–«‘∏’°“√ Crystallization ®–‰¥â

Õß»“‡´≈‡´’¬ ‡ªìπ‡«≈“ 24 ™—Ë«‚¡ß §«“¡‡¢â¡¢âπμË” º≈÷° ’‡À≈◊Õß√Ÿª‡¢Á¡ (√Ÿª∑’Ë 1) ª√‘¡“≥ 250 ¡‘≈≈‘°√—¡
ÿ¥∑’ˉ¡à‡ÀÁπ‡™◊ÈÕ·∫§∑’‡√’¬‡®√‘≠‡μ‘∫‚μ ®–‡ªìπ§à“ MBC §‘¥‡ªìπ % yield ‡∑à“°—∫ 0.4 ´÷Ë߇ªìπ≈—°…≥– ”§—≠

°“√∑¥≈Õß∑ÿ°«‘∏’®–∑”∑—ÈßÀ¡¥ 3 §√—Èß ¢Õß “√™π‘¥π’È ºŸâ«‘®—¬‰¥â∑”°“√μ√«® Õ∫§«“¡
3.3 °“√»÷°…“ƒ∑∏‘Ïμâ“π‡™◊ÈÕ·∫§∑’‡√’¬¥â«¬ ∫√‘ ÿ∑∏‘χ∫◊ÈÕßμâπ ¥â«¬«‘∏’ TLC (√Ÿª∑’Ë 2) ·≈–‰¥â
°≈âÕß®ÿ≈∑√√»πåÕ‘‡≈Á°μ√Õπ »÷°…“¢âÕ¡Ÿ≈º≈°“√æ‘ Ÿ®πå‡Õ°≈—°…≥å¢Õß “√≈Ÿªîπ‘-

°≈âÕß®ÿ≈∑√√»πåÕ‘‡≈Á°μ√Õπ À√◊Õ Trans- øÕ≈‘π ¥—ßμ“√“ß∑’Ë 1
mission electron microscope (TEM) ¡’ º≈°“√∑¥ Õ∫ƒ∑∏‘Ïμâ“π‡™◊ÈÕ B. cereus ¥â«¬
ª√–‚¬™πå„π°“√»÷°…“√Ÿª√à“ß≈—°…≥–¢Õ߇™◊ÈÕ «‘∏’ Disc diffusion «—¥§à“ inhibition zone ‰¥âº≈

·∫§∑’‡√’¬·∫∫μ—¥¢«“ß “¡“√∂¡Õ߇ÀÁπÕß§å ¥—ßμ“√“ß∑’Ë 2 æ∫«à“ “√≈Ÿªîπ‘øÕ≈‘π “¡“√∂¬—∫¬—Èß
ª√–°Õ∫∑’ˇª≈’ˬπ·ª≈ß À≈—߉¥â√—∫ “√≈Ÿªîπ‘øÕ≈‘π °“√‡®√‘≠‡μ‘∫‚μ¢Õ߇™◊ÈÕ B. cereus ‚¥¬¡’¢π“¥ in-
‰¥â·°à cell wall, cell membrane ·≈–≈—°…≥– hibition zone ∑’Ë 50 μg/disc ‡∑à“°—∫ Ampicillin
[10]
‚§√ß √â“ß¿“¬„π‡´≈≈å¢Õ߇™◊ÈÕ·∫§∑’‡√’¬ μ—«Õ¬à“ß ·≈–∑’Ë 75 μg/disc ¡’¢π“¥„À≠à°«à“
‡™◊ÈÕ·∫§∑’‡√’¬∑’Ë∑¥ Õ∫°—∫ “√μ—«Õ¬à“ß·≈â«®–≈â“ߥ⫬ ‡¡◊ËÕ∑”°“√∑¥ Õ∫ª√– ‘∑∏‘¿“æ°“√μâ“π‡™◊ÈÕ

0.1 M phosphate buffer (PBS, pH 7.2) ∑”°“√ ¥â«¬«‘∏’ Microdilution method ‰¥â§à“ MIC ‡∑à“°—∫
fixed μ—«Õ¬à“ߥ⫬ 0.25% glutaraldehyde ≈â“ߥ⫬ 8 μg/ml ·≈– §à“ MBC ‡ªìπ 16 μg/ml μ“¡≈”¥—∫
0.1 M PBS Õ’°§√—Èß ·≈â« fixed ¥â«¬ 1% osmium ¥—ßμ“√“ß∑’Ë 3

tetroxide À≈—ß®“°π—Èπ dehydrate ¥â«¬ acetone º≈°“√μ√«®¥â«¬°≈âÕß®ÿ≈∑√√»πåÕ‘‡≈Á°μ√Õπ
®“°§«“¡‡¢â¡¢âπ 20% -100% π”μ—«Õ¬à“߉ª·∑π∑’Ë ¥—ß√Ÿª∑’Ë 3 æ∫«à“ ‡´≈≈å¢Õß B. cereus ∑’Ë‡μ‘¡ “√≈Ÿªî-

¥â«¬ Spurrûs resin ‡æ◊ËÕ‡°Á∫μ—«Õ¬à“ß„ÀâÕ¬Ÿà„π·∑àß π‘øÕ≈‘π ¡’≈—°…≥–‡ª≈’Ë¬π‰ª‡¡◊ËÕ‡∑’¬∫°—∫‡´≈≈å
æ≈“ μ‘° 𔉪엥 section ¥â«¬‡§√◊ËÕß ultramicro- ·∫§∑’‡√’¬ª°μ‘∑’ˉ¡à‰¥â‡μ‘¡ “√ °—¥ ‚¥¬≈—°…≥–∑’Ë
tome „Àâμ—«Õ¬à“ßÕ¬Ÿà∫π·ºàπ copper grids ¬âÕ¡ ’ —߇°μ‡ÀÁπ ¡’√Ÿª√à“ß∫«¡ ·≈–∫“߇´≈≈å®–μ‘¥ ’‡¢â¡

¥â«¬ 2% uranyl acetate ·≈– lead citrate ·≈â« ´÷ËßÕ“®‡ªìπº≈®“°°“√∑’ˇ¬◊ËÕÀÿ⡇´≈≈å ·≈–ºπ—߇´≈≈å
μ√«®μ—«Õ¬à“ߥ⫬°≈âÕß®ÿ≈∑√√»πåÕ‘‡≈Á°μ√Õπ‡æ◊ËÕ¥Ÿ ∂Ÿ°∑”≈“¬