Page 51 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 11 ฉบับที่ 3

260 «“√ “√°“√·æ∑¬å·ºπ‰∑¬·≈–°“√·æ∑¬å∑“߇≈◊Õ° ªï∑’Ë 11 ©∫—∫∑’Ë 3°—𬓬π-∏—𫓧¡ 2556

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1
μ“√“ß∑’Ë 5 ¢âÕ¡Ÿ≈ H-NMR (500 MHz) ¢Õß 3 ‡ª√’¬∫‡∑’¬∫°—∫ Caffeic Acid
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μ”·Àπàß‚ª√μÕπ δ (ppm) „π d -DMSO ¢Õß 3 δ (ppm) „π CD OD ¢Õß caffeic acid
H 6 H 3
H-2 7.05 (d, J=1.96 Hz, 1H) 7.07 (d, J=2.0 Hz, 1H)
OH-3 9.20 (s, 1H) -
OH-4 9.60 (s, 1H) -
H-5 6.75 (d, J=8.1 Hz, 1H) 6.81 (d, J=8.2 Hz, 1H)
H-6 6.95 (dd, J=8.2, 1.9 Hz, 1H) 6.95 (dd, J=8.2, 2.0 Hz, 1H)
H-7 7.40 (d, J=15.84 Hz, 1H) 7.55 (d, J=15.9 Hz, 1H)
H-8 6.15 (d, J=15.85 Hz, 1H) 6.24 (d, J=15.9 Hz, 1H)
OH-9 12.15 (s, 1H) -

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μ“√“ß∑’Ë 6 ¢âÕ¡Ÿ≈ C-NMR (125 MHz) ¢Õß 3 ‡ª√’¬∫‡∑’¬∫°—∫ Caffeic Acid
13
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μ”·Àπàߧ“√å∫Õπ δ (ppm) „π d -DMSO ¢Õß 3 δ (ppm) „π CD OD ¢Õß caffeic acid
C 6 C 3
C-1 126.17 128.3
C-2 115.10 115.7
C-3 146.02 147.2
C-4 148.59 149.8
C-5 116.21 117.0
C-6 121.59 123.4
C-7 145.02 147.6
C-8 115.59 116.0
C-9 168.33 171.6

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‡Õ (Flu A/H3) ¥â«¬«‘∏’ Plaque Reduction Assay ‰¥â·°à ƒ∑∏‘Ïμâ“π‡™◊ÈÕ√“ ‡√‘¡ ¡“≈“‡√’¬ «—≥‚√§ cyto-

æ∫«à“ “√ °—¥À¬“∫·≈– “√∫√‘ ÿ∑∏‘Ï 1-3 ¡’ƒ∑∏‘Ϭ—∫¬—Èß toxic ·≈–μâ“πÕ—°‡ ∫ ∑’˧«“¡‡¢â¡¢âπμà“ßÊ π—Èπ æ∫«à“
Flu A (μ“√“ß∑’Ë 8) à«πƒ∑∏‘Ï°“√¬—∫¬—È߇™◊ÈÕ‡Õ¥ å (HIV- rosmarinic acid ·≈– caffeic acid · ¥ßƒ∑∏‘Ï

1) ¡’‡°≥±å„π°“√μ—¥ ‘π§◊Õ ‰¡à„Àâμ‘¥‡™◊ÈÕ„π‡´≈≈å∑’Ë ‡©æ“–°“√¬—∫¬—È߇™◊ÈÕ«—≥‚√§ (H37Ra strain) ‡∑à“π—Èπ
„™â∑¥ Õ∫‰¥â¡“°°«à“À√◊Õ‡∑à“°—∫ 90% ¢÷Èπ‰ª ®÷ß®– ‚¥¬¡’§«“¡‡¢â¡¢âπμË” ÿ¥∑’Ë “¡“√∂¬—∫¬—È߇™◊ÈÕ‰¥â
∂◊Õ«à“¡’ƒ∑∏‘Ϭ—∫¬—Èß HIV-1 ‰¥â„πÀ≈Õ¥∑¥≈Õß ‚¥¬ (MIC) ‡∑à“°—∫ 200 ·≈– 100 ‰¡‚§√°√—¡/¡‘≈≈‘≈‘μ√
„™â«‘∏’ Immuno-fluorescence Assay æ∫«à“ “√ μ“¡≈”¥—∫ ‚¥¬ “√§«∫§ÿ¡∫«° (positive control)

°—¥À¬“∫·≈– “√∫√‘ ÿ∑∏‘Ï 1-3 ¡’ƒ∑∏‘Ϭ—∫¬—Èß HIV-1 §◊Õ isoniazid ¡’§à“ MIC ‡∑à“°—∫ 0.250 ‰¡‚§√°√—¡/
(μ“√“ß∑’Ë 9) ¡‘≈≈‘≈‘μ√