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ÒˆÙ «“√ “√°“√·æ∑¬ ¬·º√ “√ · °“√≈– ∑¬åå∑“߇≈◊Õ° ªï∑’Ë ¯©∫—∫∑’Ë Ú-Û æƒ…¿“§¡-∏—𫓧¡ ÚııÛ
∑“
°“√ ∑ å π‰∑¬
«“√ “√°“√·æ∑¬å·ºπ‰∑¬·≈–°“√·æ∑¬
√ “√°“√ ∑¬å·ºπ‰∑¬·≈–°“√ ∑¬å∑“å·ºπ‰∑¬·≈–°“√·æ∑¬å∑“߇≈◊Õ°
ethyl acetate ‰¥â “√ °—¥·¬° à«π Ab-E1, Ab-E2, Ab- (GC-5) πÈ”Àπ—° ˆ˘.Ú, ¯ı.ˆ˜, Ò˘ˆ.˜ı ·≈– ÙÛ.˘ °√—¡

E3, Ab-E4, ·≈– Ab-E5 πÈ”Àπ—° Ù.ÒıÛ, .˘Û¯ı, μ“¡≈”¥—∫ (§‘¥‡ªìπ %yield ‡∑à“°—∫ Ò˜.ıÛ, ÚÒ.˜, Ù˘.¯ı
.ˆˆÙ, .ıÛÛˆ ·≈– Ò.ÚˆÚÒ °√—¡ (§‘¥‡ªìπ %yield ·≈– Ò.˘Ò μ“¡≈”¥—∫ ‡¡◊ËÕ‡∑’¬∫°—∫πÈ”Àπ—° “√ °—¥‡Õ∑“πÕ≈
‡∑à“°—∫ Ú, Ù, Û, Ú ·≈– ıÒ.ÛÒ ‡¡◊ËÕ‡∑’¬∫°—∫πÈ”Àπ—° “√ ·≈–§‘¥‡ªìπ %yield ‡∑à“°—∫ Û.ˆÙ, Ù.ıÒ, Ò.Ûˆ ·≈– Ú.Ú˜

°—¥‡Õ∑“πÕ≈) μ“¡≈”¥—∫ ‡¡◊ËÕ‡∑’¬∫°—∫πÈ”Àπ—°„∫™–¡«ß·Àâß)
Ú.ˆ “√ °—¥¢à“ ¥â«¬«‘∏’ supercritical fluid ex- Ú.¯ “√ °—¥¬Õ
tractor (SFE) π”ºß„∫¬Õ·Àâß¡“ °—¥¥â«¬ ˘ı% ‡Õ∑“πÕ≈ ‚¥¬«‘∏’

π”ºß¢à“·Àâß πÈ”Àπ—° ÚÛ °√—¡ ¡“ °—¥¥â«¬«‘∏’‡§√◊ËÕß soxhlet extraction π” “√ °—¥∑’ˉ¥â¡“√–‡À¬¥â«¬‡§√◊ËÕß
°—¥ “√·∫∫Õ—μ‚π¡—μ‘ ‚¥¬„™âμ—«∑”≈–≈“¬§◊Õ §“√å∫Õπ‰¥- √–‡À¬ ÿ≠≠“°“»‰¥â “√ °—¥‡Õ∑“πÕ≈ (MC-EC) πÈ”Àπ—°

ÕÕ°‰´¥å (CO ) °—¥‡ªìπ‡«≈“ª√–¡“≥ ˆ ™—Ë«‚¡ß ∑’˧«“¡¥—π Ûˆ.¯ı °√—¡ (§‘¥‡ªìπ %yield ‡∑à“°—∫ ÚÒ.ˆ¯ ‡¡◊ËÕ‡∑’¬∫πÈ”
2
Û psi Õÿ≥À¿Ÿ¡‘ Ùı˚´ ‚¥¬„™â√–∫∫‰¥π“¡‘§ ‰¥â “√ °—¥πÈ” Àπ—°„∫¬Õ·Àâß)
Àπ—° ÒÚ.ı °√—¡ (§‘¥‡ªìπ %yield ‡∑à“°—∫ ı.ÙÛ ‡¡◊ËÕ‡∑’¬∫ Ú.˘ “√ °—¥ªí≠®¢—π∏å

°—∫πÈ”Àπ—°¢à“·Àâß) ®“°π—Èπ‡°Á∫ “√ °—¥∑’ˉ¥â‰«â„π¢«¥ ’™“∑’Ë π”ºß·ÀâߢÕß à«π„μ⥑πªí≠®¢—π∏å πÈ”Àπ—° Ú.˜
o
Õÿ≥À¿Ÿ¡‘ Ú-Ù ´ °àÕππ”¡“«‘‡§√“–ÀåÀ“Õߧåª√–°Õ∫∑“߇§¡’ °‘‚≈°√—¡ ¡“ °—¥¥â«¬ ˘ı% ‡Õ∑“πÕ≈ ‚¥¬«‘∏’ soxhlet ex-

·≈–°“¬¿“æÀ√◊Õ‡μ√’¬¡ “√ °—¥‡æ◊ËÕ°“√∑¥ Õ∫ƒ∑∏‘Ϭ—∫¬—Èß traction ®“°π—Èππ” “√ °—¥∑’ˉ¥â‰ª∑”„Àâ·Àâߥ⫬‡§√◊ËÕß
o
°“√∑”ß“π¢Õ߇Õπ‰´¡å pancreatic lipase ·≈– HMG-CoA √–‡À¬ ÿ≠≠“°“»∑’ËÕÿ≥À¿Ÿ¡‘ Ù-Ùı ´ ‰¥â “√ °—¥‡Õ∑“πÕ≈
reductase (GP-E) πÈ”Àπ—° Òı.˘˘ °√—¡ (§‘¥‡ªìπ %yield ‡∑à“°—∫ ı.ı˘

«‘∏’°“√·¬° “√∫√‘ ÿ∑∏‘Ï¢à“ · ¥ß¥—ß·ºπ¿Ÿ¡‘∑’Ë Ò ‡¡◊ËÕ‡∑’¬∫°—∫πÈ”Àπ—°ªí≠®¢—π∏å·Àâß)
Ú.˜ “√ °—¥™–¡«ß Û. °“√‡μ√’¬¡ “√ °—¥‡æ◊ËÕ°“√∑¥ Õ∫ƒ∑∏‘Ϭ—∫¬—Èß°“√
°—¥ºß„∫™–¡«ß¥â«¬ ˘ı% ‡Õ∑“πÕ≈ ¥â«¬«‘∏’ ∑”ß“π¢Õ߇Õπ‰´¡å pancreatic lipase ·≈–‡Õπ‰´¡å HMG-

soxhlet extraction ®“°π—Èππ” “√ °—¥∑’ˉ¥â‰ª√–‡À¬¥â«¬ CoA reductase
‡§√◊ËÕß√–‡À¬ ÿ≠≠“°“»‰¥â “√ °—¥‡Õ∑“πÕ≈ (GC-1) ‡μ√’¬¡ ¡ÿπ‰æ√ ˘ ™π‘¥ ‰¥â·°à ∫—«À≈«ß Ω√—Ëß ¬à“π“ß ¡–√ÿ¡
π” “√ °—¥‡Õ∑“πÕ≈ (GC-1) ¡“≈–≈“¬¥â«¬ ˆ% μ–≈‘ߪ≈‘ß ¢à“ ™–¡«ß ªí≠®¢—π∏å ·≈–¬Õ (√«¡‡ªìπ “√ °—¥

‡Õ∑“πÕ≈ ®“°π—Èπ·¬°‡ªìπ “√ °—¥¬àÕ¬‚¥¬°“√ partition ¥â«¬ ®”π«π Ú μ—«Õ¬à“ß) ‡æ◊ËÕ∑¥ Õ∫ƒ∑∏‘Ϭ—∫¬—Èß°“√∑”ß“π¢Õß
hexane, dichloromethane (CH Cl ) ·≈– n-butanol, ‡Õπ‰´¡å pancreatic lipase ·≈–‡μ√’¬¡ ¡ÿπ‰æ√ ı ™π‘¥ ‰¥â·°à
2 2
(BuOH) μ“¡≈”¥—∫ π” “√ °—¥·μà≈– à«π ∑’ˉ¥â‰ª√–‡À¬¥â«¬ ∫—«À≈«ß Ω√—Ëß μ–≈‘ߪ≈‘ß ™–¡«ß ·≈–¬Õ (√«¡‡ªìπ “√ °—¥
‡§√◊ËÕß√–‡À¬ ÿ≠≠“°“»‰¥â “√ °—¥ hexane (GC-2), “√ °—¥ ®”π«π Òˆ μ—«Õ¬à“ß) ∑¥ Õ∫ƒ∑∏‘Ϭ—∫¬—Èß°“√∑”ß“π¢Õ߇Õπ‰´¡å
CH Cl (GC-3), “√ °—¥ BuOH (GC-4) ·≈– “√ °—¥πÈ” HMG-CoA reductase „ÀâÕ¬Ÿà„π√Ÿª∑’Ë≈–≈“¬πÈ”‰¥â ‚¥¬
2 2

“√ °—¥¢à“πÈ”Àπ—° ı.Úı °√—¡ (AG-SFE)
Acetone : Methanol column chromatography
W W W W
Fr.1 Fr. 2 Fr. 3 Fr. 4
πÈ”Àπ—° Ú.ÒÛ˜Ú °√—¡ πÈ”Àπ—° Ò ¡‘≈≈‘°√—¡ πÈ”Àπ—° Ú.˘˘Ú¯ °√—¡ πÈ”Àπ—° .ÚÚ °√—¡
(AG-H) ( “√∫√‘ ÿ∑∏‘Ï) (AG-A) (AG-M)
(AG)

·ºπ¿Ÿ¡‘∑’Ë Ò °“√·¬° “√∫√‘ ÿ∑∏‘Ï¢à“