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Journal of Thai Traditional & Alternative Medicine Vol. 8 Nos. 2-3 May-December 2010 ÒˆÛ
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μ“√“ß∑’Ë Ò · ¥ß°“√‡μ√’¬¡ ¡ÿπ‰æ√ ®”π«π ˘ ™π‘¥
≈”¥—∫ ¡ÿπ‰æ√ ™◊ËÕ«‘∑¬“»“ μ√å ™◊ËÕ«ß»å πÈ”Àπ—° Õÿ≥À¿Ÿ¡‘ √–¬–‡«≈“ πÈ”Àπ—° §‘¥‡ªìπ√âÕ¬≈–¢Õß
∑’Ë ( ¥) (Õß»“ „π°“√Õ∫ (·Àâß) º≈º≈‘μ (% yield)
°‘‚≈°√—¡ ‡´≈‡´’¬ ) (™—Ë«‚¡ß) °‘‚≈°√—¡‡∑à“°—∫‡¡◊ËÕ‡∑’¬∫°—∫
πÈ”Àπ—° ¡ÿπ‰æ√ ¥

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Ò ∫—«À≈«ß Nelumbo nucifera Gaertn. Nelumbonaceae ˜. Ùı-ı ˜Ú Ò.Úı Ò˜.¯
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Ú Ω√—Ëß Psidium guajava L. Myrtaceae .Û Ùı-ı Ù¯ .Òı ı.
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Û ¬à“π“ß Tiliacora triandra (Colebr.) Diels Menispermaceae Ú.Ò Ùı-ı ˜Ú .Ùˆ ÚÒ.˘
˜
Ù ¡–√ÿ¡ Moringa oleifera Lamk. Moringaceae - - - .Ò -
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ı μ–≈‘ߪ≈‘ß Averrhoa bilimbi L. Oxalidaceae Ú. Ùı-ı Ù¯ .ÛÒ Òı.ı
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ˆ ¢à“ Alpinia galanga (L) Willd. Zingiberaceae Ù. Ùı-ı ˜Ú .ÙÛ Ò.˜
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˜ ™–¡«ß Garcinia cowa Roxb. ex DC. Clusiaceae Ò. Ùı-ı Ù¯ Ò.˘ Ò˘.
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¯ ¬Õ Morinda citrifolia L. Rubiaceae .˘ Ùı-ı Ù¯ .Ò˜ Ò¯.¯
¯,˘
˘ ªí≠®¢—π∏å Gynostemma pentaphyllum Cucurbitaceae Ò. Ùı-ı Ù¯ Ò. Ò.
(Thunb.) Maniko.
À¡“¬‡Àμÿ Ò. à«π∑’Ë„™â¢Õß ¡ÿπ‰æ√∑—Èß ˘ ™π‘¥§◊Õ „∫ ¬°‡«âπ ¢à“ „™â à«π‡Àßâ“
Ú. - À¡“¬∂÷ß ‡μ√’¬¡«—μ∂ÿ¥‘∫„∫¡–√ÿ¡·Àâß

Ú.Û “√ °—¥¬à“π“ß ‚¥¬«‘∏’√’ø≈—°´å (reflux) °√Õß “√ °—¥∑’ˉ¥â √–‡À¬¥â«¬‡§√◊ËÕß

π”ºß„∫¬à“π“ß∑’Ë∫¥≈–‡Õ’¬¥πÈ”Àπ—° Ú¯ˆ °√—¡ √–‡À¬ ÿ≠≠“°“»„Àâ “√ °—¥‡¢â¡¢âπ¢÷Èπ ·≈â«π”‰ª∑”„Àâ·Àâß
¡“ °—¥¥â«¬ ˘ı% ‡Õ∑“πÕ≈ ¥â«¬«‘∏’ soxhlet extraction ¥â«¬‡§√◊ËÕß∑”§«“¡‡¬Áπ‰¥â “√ °—¥¥â«¬πÈ” (MO-2) πÈ”Àπ—°

®“°π—Èππ” “√ °—¥∑’ˉ¥â‰ª√–‡À¬¥â«¬‡§√◊ËÕß√–‡À¬ ÿ≠≠“°“» Û¯.ˆ˜ °√—¡ (§‘¥‡ªìπ %yield ‡∑à“°—∫ Û¯.ˆ˜ ‡¡◊ËÕ‡∑’¬∫°—∫
‰¥â “√ °—¥πÈ”Àπ—° ı˜.˘ı °√—¡ (TT1) (§‘¥‡ªìπ %yield ‡∑à“°—∫ πÈ”Àπ—°ºß„∫¡–√ÿ¡·Àâß)
ÚÒ.˘ ‡¡◊ËÕ‡∑’¬∫°—∫πÈ”Àπ—°¬à“π“ß·Àâß) Ú.ı “√ °—¥μ–≈‘ߪ≈‘ß

π”ºß„∫¬à“π“ß∑’Ë∫¥≈–‡Õ’¬¥πÈ”Àπ—° Ò °√—¡ ¡“ π”ºß„∫μ–≈‘ߪ≈‘ß·Àâß πÈ”Àπ—° Ú °√—¡ ¡“ °—¥
°—¥¥â«¬°“√√’ø≈—°´å ®”π«π Ú §√—Èß π” à«π„ ∑’ˉ¥â®“°°“√ ¥â«¬πÈ” ‚¥¬«‘∏’√’ø≈—°´å °√Õß “√ °—¥∑’ˉ¥â·≈–√–‡À¬¥â«¬
°√Õ߉ª√–‡À¬¥â«¬‡§√◊ËÕß√–‡À¬ ÿ≠≠“°“» ·≈–∑”„Àâ·Àâß ‡§√◊ËÕß√–‡À¬ ÿ≠≠“°“»„Àâ “√ °—¥‡¢â¡¢âπ¢÷Èπ ·≈â«π”‰ª∑”„Àâ

¥â«¬‡§√◊ËÕß Freeze dryer ‰¥âπÈ”Àπ—° “√ °—¥ ÚÛ.ıı °√—¡ ·Àâߥ⫬‡§√◊ËÕß Freeze dryer ‰¥â “√ °—¥πÈ” (Ab-W) πÈ”Àπ—°
(TTW) (§‘¥‡ªìπ %yield ‡∑à“°—∫ ÚÛ.ıı ‡¡◊ËÕ‡∑’¬∫°—∫πÈ” ıÛ.Ûı °√—¡ (§‘¥‡ªìπ %yield ‡∑à“°—∫ Úˆ.ˆ¯ ‡¡◊ËÕ‡∑’¬∫°—∫
Àπ—°¬à“π“ß·Àâß) πÈ”Àπ—°„∫μ–≈‘ߪ≈‘ß·Àâß)

Ú.Ù “√ °—¥¡–√ÿ¡ π”ºß„∫μ–≈‘ߪ≈‘ß·ÀâßπÈ”Àπ—° ÛÚ¯ °√—¡ ¡“ °—¥¥â«¬
“√ °—¥ MO-1 ˘ı% ‡Õ∑“πÕ≈ ‚¥¬«‘∏’°“√ soxhlet extraction ®“°π—Èππ”

π”ºß„∫¡–√ÿ¡·Àâß πÈ”Àπ—° Ò °√—¡ °—¥¥â«¬ ˘ı% ‰ª∑”„Àâ·Àâߥ⫬‡§√◊ËÕß√–‡À¬ ÿ≠≠“°“» ∑’ËÕÿ≥À¿Ÿ¡‘ Ù-ı˚´
‡Õ∑“πÕ≈‚¥¬«‘∏’ soxhlet extraction π” “√ °—¥∑’ˉ¥â¡“ ‰¥â “√ °—¥‡Õ∑“πÕ≈ (Ab-E) πÈ”Àπ—° ıÛ.Ûı °√—¡ (§‘¥‡ªìπ
√–‡À¬¥â«¬‡§√◊ËÕß√–‡À¬ ÿ≠≠“°“»‰¥â “√ °—¥‡Õ∑“πÕ≈ (MO- %yield ‡∑à“°—∫ Û˘.ÙÙ ‡¡◊ËÕ‡∑’¬∫°—∫πÈ”Àπ—°„∫μ–≈‘ߪ≈‘ß·Àâß)

1) πÈ”Àπ—° ÛÚ.Ú˜ °√—¡ (§‘¥‡ªìπ% yield ‡∑à“°—∫ ÛÚ.Ú˜ π” “√ °—¥ Ab-E πÈ”Àπ—° Ú °√—¡ ¡“·¬°‚¥¬ col-
‡∑’¬∫πÈ”Àπ—°ºß„∫¡–√ÿ¡·Àâß) umn chromatography ‚¥¬«—Ø¿“§§ß∑’Ë (stationary phase)

“√ °—¥ MO-2 §◊Õ silica gel G60 ¢π“¥ .Ù-.ˆÛ ¡¡. ·≈–«—Ø¿“§
π”ºß„∫¡–√ÿ¡·Àâß πÈ”Àπ—° Ò °√—¡ °—¥¥â«¬πÈ” ‡§≈◊ËÕπ∑’Ë (mobile phase) §◊Õ à«πº ¡¢Õß acetone °—∫