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OP-19

Suppression of phagocytic activity of Thai vegetables in mouse peritoneal

macrophages

1 2 3 3 2
Leekhaosoong K , Kukongviriyapan U , Kukongviriyapan V , Kongyingyoes B , Kantip S.
1
College of Thai Traditional and Alternative Medicine, BURU.
2 3
Department of Physiology and Department of Pharmacology, Faculty of Medicine, KKU.

Rational: Many Thai vegetables have been used as medicinal plants in Thai folk medicine. Although Thai

vegetables are an important source of essential nutrients, there are very limited scientific evidences supporting
their health benefits beyond the nutrient contribution.
Objective: The present study is aimed to investigate the phagocytic activity of Thai local vegetables in

mouse peritoneal macrophages stimulated with zymosan and antioxidant compounds by determination of the
total phenols in these vegetable extracts. Five types of Thai local vegetables were selected and used in this

study, including Meg (Eugenia grata), Gra-don (Barringtonia acutangula), Ka-Yang (Limnophila aromatica),
Teawdang (Cratoxylum subsp) and Kae flower (Sesbania grandiflora).
Methodology: All vegetables were collected from the agricultural farm in Khon Kaen province, and freshly

extracted with water. The amount of total phenols of vegetable extracts was assessed by using Folin-Ciocalteu
colorimetric assay. The phagocytic capacity of mouse macrophages was indicated by the number of macroph-

ages engulfs zymosan particles (modified from Courreges and Benenciaûs study).
Results: It was found that all of the vegetable extracts significantly suppressed the phagocytotic activity
of mouse macrophages in a dose-dependent manner with IC ranging from 453 to 1,900 μg / ml. Moreover, the
50
amount of total phenols found in these vegetable extracts was about 1-32 g of gallic acid equivalent (GAE) / 100
g extract.
Conclusion: As the phagocytosis of macrophages is usually accompanied by an oxidative burst, therefore,

the antiphagocytic capability of these vegetable extracts might be due to their antioxidant activities.
Key Words: Thai vegetables, Macrophages, Phagocytosis, Antioxidants.
Acknowledgments: The research work was supported by a grant from the Thai Traditional Medicine

Development Foundation and grant in aid from Khon Kaen University Graduate Research Fund.