Page 107 - journal-6-2-S-Full

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In vivo anti-inflammatory of the 7 Lanna medicinal plant recipes selected

from the Thai/Lanna Medicinal Plant Recipe Database (T/LMPRD)

1,2 2 2 1.2
Aranya Manosroi , Suda Saowakhon , Rattanapon Chantip , Jiradej Manorsoi
1
Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200 Thailand
2
Natural Products Research and Development Center (NPRDC), Institute for Science and Technology Research and Development,
Chiang Mai University, Chiang Mai 50000 Thailand

Rationale: In the northern part of Thailand, numbers of Lanna medicinal plants have been used for foods, drugs,
cosmetics and traditional ceremony. Combination of medicinal plants has usually been used as folklore medicinal
recipes for the treatment of several diseases or symptoms, such as inflammation, cancer and pain. Recipes which
have been proved to be therapeutically effective were recorded in Pub Sa (mulberry pulp paper), Bai Laan (palm
leaf) and Sumud Khoi (Siamese rough bush paper). Natural Products Research and Development Center (NPRDC)
has prepared the Thai/Lanna Medicinal Plant Recipe Database (T/LMPRD) and the recipes with anti-inflammatory
activity were screened for further development to anti-inflammatory products.

Objective: This study has aimed to screen the anti-inflammatory activities of the selected Thai Lanna medicinal
plant recipes in rats by the hind paw edema assay

Methodology: Seven Thai/Lanna medicinal plant recipes were selected by frequency and scientific data of the
medicinal plants from the Thai/Lanna Medicinal Plant Recipe Database: Recipes-Disease-Medicinal Plants (T/
LMPRD) developed by Natural Products Research and Development Center (NPRDC). The plants were washed
o
with distilled water, chopped into small pieces, dried in oven (50 C) and then powdered. The powder of each plant
was combined and mixed according to the recipe and extracted by boiling in distilled water for 30 min, filtered,
o
then evaporated and stored at 4 C prior to use. Male and female Sprague-Dawley rats (200-250 g body weight)
were divided into 38 groups of 6 animals in each group. In the treated group, the animals were injected
subcutaneously into the plantar side of the hind paw with 0.1 ml of 1% carrageenan suspension (λ type IV Sigma).
The extract was dissolved in distilled water, and administered orally (not more than 1 ml.) 30 min prior
carrageenan injection. Rat paw edema was determined by volume displacement method (plethysmometer, Ugo
Basile 7150) at 0 hr (just before) and at 1, 2, and 3 hr after carrageenan injection. The percentages of paw edema
inhibition of the extract from Thai/Lanna medicinal plant recipes were compared with prednisolone acetate, a
positive control.
Results: The anti-inflammatory activity of the extract from Thai/Lanna medicinal plant recipes Nos. 2, 3 and 4
have the maximum percentage inhibition of 79.98±11.58%, 74.38±16.14% and 76.76±27.32% compared with the
control, 2 hrs after carrageenan injection at the 3 doses of 1.60, 21.23 and 3.24 mg/kg, respectively. Prednisolone
acetate at the dose of 4 mg/kg gave the maximum percentage hind paw edema inhibition at 31.67±8.53% compared
with the control. The maximum percentages of inhibition of recipe Nos. 2, 3 and 4 were higher than prednisolone
acetate (4 mg/kg body weight at 2 hrs) of about 2.53, 2.35 and 2.42 times, respectively.

Conclusion: This study has demonstrated that recipe No. 2 (containing Zingiber officinale Roscoe, Vitex trifolia
L., Sesamum orientale L.) showed the maximum percentage hind paw edema inhibition of 79.98±11.58% compared
with the control at the dose of 1.60 mg/kg, at 2 hrs after carrageenan injection. This recipe gave higher anti-
inflammatory activity than prednisolone acetate (4 mg/kg body weight at 2 hrs) of about 2.53 times. This recipe
will be selected for further development of anti-inflammatory products.