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80 «“√ “√°“√·æ∑¬å·ºπ‰∑¬·≈–·æ∑¬å∑“߇≈◊Õ° ªï∑’Ë Ù ©∫—∫∑’Ë Ò µÿ≈“§¡ ÚıÙ¯-¡°√“§¡ ÚıÙ˘

¬“«¬Õ¥À≈—°‡©≈’ˬ ‡ªÕ√凴Áπµåµâπ∑’Ë Ÿß‡°‘π 3 ´¡. ‡©≈’ˬ ·≈–®”π«π√“°‡©≈’ˬ Ÿß∑’Ë ÿ¥‡∑à“°—∫ 4.2 ´¡.,

92.8 % ·≈– 7.4 √“° µ“¡≈”¥—∫ ∑”„À≥âµâπ∑’Ë ¡∫Ÿ√≥å°«à“ ‡¡◊ËÕπ”µâπÕàÕπÕÕ°ª≈Ÿ°„π‡√◊Õπ‡æ“–™”
¡’‡ªÕ√凴Áπµå√Õ¥™’«‘µ‡©≈’ˬ 94.25 % ‡¡◊ËÕª≈Ÿ°‰¥â 1 ‡¥◊Õπ ‡¡◊ËÕπ”¬Õ¥ÕàÕπªí≠®¢—π∏å “¬æ—π∏ÿå®’π·≈–
“¬æ—π∏ÿå‰∑¬¡“‡æ“–‡≈’Ȭß∫π Ÿµ√Õ“À“√ MS58 ∑’Ë¡’À√◊Õ‰¡à¡’ Chitosan 0.1 % ¢π“¥§«“¡‡¢â¡¢âπ 5
¡≈./≈. (C5) ¡’§à“ total saponin ¢Õ߬եÕàÕπªí≠®¢—π∏å “¬æ—π∏ÿå®’π∫π Ÿµ√Õ“À“√ MS58 ·≈–

MS58+C5 ‡∑à“°—∫ 5.10 % ·≈– 5.08 % ·≈–¢Õ߬եÕàÕπªí≠®¢—π∏å “¬æ—π∏ÿå‰∑¬‡∑à“°—∫ 5.21 % ·≈–
5.45 % µ“¡≈”¥—∫ µâπªí≠®¢—π∏å “¬æ—π∏ÿå‰∑¬∑’˪≈Ÿ°„π‡√◊Õπ‡æ“–™”‡ªìπ‡«≈“ 3 ‡¥◊Õπ ¡’§à“ total

saponin ෈ҡѺ 6.67 %

ABSTRACT
Shootlets from Gynostemma pentaphyllum (Thunb.) Makino were grown on

MS medium supplemented with hormone BAP and NAA or KN and 2,4D or KN and IAA
and GA with concentration of 0.1 - 9 mg/lit for 1 month. The multiple shoots were grown.
The best multiple shoots was on MS medium supplemented with BAP, GA and citric acid

with the concentration of 0.1 mg/l , 5 mg/l and 150 mg/l respectively and increase the iron
in MS medium for equal amount (MS58) in one month. The average of multiple shoots
was 4.3 shoots, the average length of shoots was 2.4 cm. When the shootlets were grown on

MS medium supplemented with hormone NAA , IAA and IBA with the concentration of
0.1, 1 and 2 mg/l for rooting medium in one to one and a half month. The best medium
was MS medium supplemented with IAA 0.1 mg/l having the average percentage of

rooting 85 % in one month of culture and 93.3 % in one and a half month of culture.
When culture for one and a half month hormone IAA 1 mg/l is better than hormone IAA
0.1 mg/l that has average main shoot length , average shoot higher than 3 cm. and average
amount of roots equal to 4.2 cm. 92.8 % and 7.4 roots respectively. There fore the plantlets

are more healthy. The average percentage of survival of plantlets when growing in nursery
for 1 month was 94.25 %. Total saponin content of multiple shoots from Chinese and
Thai variety on MS58 and MS58+C5 medium were 5.10 % and 5.08 % for Chinese variety,

5.21 % and 5.45 % for Thai variety respectively. The total saponin for three months of Thai
variety plants growing in the nursery was 6.67 %.

Key Words : Gynostemma pentaphyllum , Tissue culture , Total saponin.