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              √âÕπ∑’ËÕÿ≥À¿Ÿ¡‘ 50 Õß»“‡´≈‡´’¬  ‡ªìπ‡«≈“ 8  sium ribbon, ¢«¥™—Ëß, ∂⫬°√–‡∫◊ÈÕß, ∂⫬√–‡À¬,

              ™—Ë«‚¡ßμàÕ«—π π“π 3 «—π ®“°π—Èππ”¡“∫¥‡ªìπºß  ‚∂¥Ÿ¥§«“¡™◊Èπ, ¢«¥ª√—∫ª√‘¡“μ√ ¢π“¥ 2 ¡‘≈≈‘≈‘μ√
              ≈–‡Õ’¬¥ ºà“π·√à߇∫Õ√å 180 ·≈⫇°Á∫„π¢«¥·°â« ’™“  (Pyrex, USA), pasture pipette

              ªî¥ π‘∑  à«πº≈¡–μŸ¡·Àâß π”¡“Õ∫‰≈৫“¡™◊Èπ„π
                                                          2. «‘∏’°“√»÷°…“
              μŸâÕ∫√âÕπ∑’ËÕÿ≥À¿Ÿ¡‘ 50 Õß»“‡´≈‡´’¬  ‡ªìπ‡«≈“ 1
              ™—Ë«‚¡ß ·≈â«π”¡“∫¥‡ªìπºß≈–‡Õ’¬¥ ºà“π·√à߇∫Õ√å 180  2.1 °“√μ√«®‡Õ°≈—°…≥å∑“߇§¡’ (Chemical
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              ·≈⫇°Á∫„π¢«¥·°â« ’™“ªî¥ π‘∑                Identification)
                   1.2  “√¡“μ√∞“π imperatorin §«“¡                1) °“√μ√«® Õ∫‡∫◊ÈÕßμâπ ‚¥¬ªØ‘°‘√‘¬“

              ∫√‘ ÿ∑∏‘Ï ≥ 98.0% (Sigma, India)            °“√‡°‘¥ ’
                   1.3  “√‡§¡’·≈–μ—«∑”≈–≈“¬ chloroform AR         °“√‡μ√’¬¡ “√≈–≈“¬μ—«Õ¬à“ß
              (RCI Labscan Limited, Thailand), diethyl ether      ™—Ëߺߡ–μŸ¡ 0.5 °√—¡ „ à„π round bot-

              (Merck, Germany), hexane (Merck, Germany),  tom flask ‡μ‘¡‡Õ∑“πÕ≈ 95% ª√‘¡“μ√ 20 ¡‘≈≈‘≈‘μ√
              methanol AR (Merck, Germany), toluene        °—¥‚¥¬«‘∏’μâ¡ °—¥ (reflux) ‡ªìπ‡«≈“ 10 π“∑’
              (Fisher Scientific, UK), ethanol, DI water, hy-  °√Õߺà“π°√–¥“…°√Õß π” “√≈–≈“¬∑’Ë°√Õ߉¥â‰ª

              drochloric acid (Merck, Germany), sulfuric acid  ∑”°“√∑¥ Õ∫μàÕ„π¢âÕ °.-ß.
              (Labscan, Thailand), ferric chloride (BDH, UK),     °. Liebermann Burchard test ‚¥¬
              potassium permanganate (BDH, UK)            π” “√≈–≈“¬μ—«Õ¬à“ß ª√‘¡“μ√ 2 ¡‘≈≈‘≈‘μ√ ¡“√–‡À¬

                   1.4 ‡§√◊ËÕß¡◊Õ ‡§√◊ËÕß∫¥ ¡ÿπ‰æ√, ‡§√◊ËÕß·√àß  ·Àâß∫πÕà“ß՗߉ÕπÈ” ®“°π—Èπ≈–≈“¬°≈—∫¥â«¬·Õ´‘μ‘°
              (Laboratory Test Sieve, UK), μŸâÕ∫√âÕπ      ·Õπ‰Œ‰¥√¥å 0.5 ¡‘≈≈‘≈‘μ√ ¥Ÿ¥ “√≈–≈“¬„ àÀ≈Õ¥

              (Memmert, Germany), ‡§√◊ËÕß™—Ë߉øøÑ“ §«“¡   ∑¥≈Õß ®“°π—Èπ ‡μ‘¡°√¥°”¡–∂—πÕ¬à“ß™â“ Ê ª√‘¡“μ√
              ≈–‡Õ’¬¥ 0.1 ¡‘≈≈‘°√—¡ (Mettler-Toledo, Switzer-  1 ¡‘≈≈‘≈‘μ√ ®π‡ÀÁπ‡ªìπ Õß™—Èπ  —ß‡°μ√Õ¬μàÕ
              land), ‡μ“‡º“Õÿ≥À¿Ÿ¡‘ Ÿß (Thermolyne, USA), UV  √–À«à“ß “√≈–≈“¬∑—Èß Õß™—Èπ

              cabinet (CAMAG, Switzerland), TLC plate             ¢. À¬¥ “√≈–≈“¬‡øÕ√‘°§≈Õ‰√¥å 1%
              heater (CAMAG, Switzerland), Õà“ßπÈ”√âÕπ, ™ÿ¥  ®”π«π 2 - 3 À¬¥ ≈ß„π “√≈–≈“¬μ—«Õ¬à“ß ª√‘¡“μ√

               °—¥ “√ ª√–°Õ∫¥â«¬ round bottom flask, heat-  1 ¡‘≈≈‘≈‘μ√  —߇°μ ’∑’ˇª≈’ˬπ·ª≈߉ª
              ing mantle, condenser ·≈–‡§√◊ËÕß∑”πÈ”‡¬Áπ           §. Shinodaûs test π” “√≈–≈“¬μ—«Õ¬à“ß
              À¡ÿπ‡«’¬π                                   ª√‘¡“μ√ 2 ¡‘≈≈‘≈‘μ√ ¡“‡μ‘¡°√¥‡°≈◊Õ‡¢â¡¢âπ 2 - 3

                   1.5 «— ¥ÿ«‘∑¬“»“ μ√å·≈–‡§√◊ËÕß·°â« TLC  À¬¥ ·≈â«‡μ‘¡≈«¥·¡°π’‡´’¬¡™‘Èπ‡≈Á° Ê 3 - 4 ™‘Èπ
              plate ™π‘¥ Silica gel GF254 (E. Merck, Ger-   —߇°μ ’¢Õß “√≈–≈“¬∑’ˇ°‘¥¢÷Èπ
              many), °√–¥“…°√Õß ‡∫Õ√å 4 (Whatman, UK),            ß. À¬¥ “√≈–≈“¬‚æ·∑ ‡´’¬¡‡ªÕ√å-

              °√–¥“…°√Õߪ√“»®“°‡∂â“ ‡∫Õ√å 41 (Whatman,    ·¡ß°“‡πμ ®”π«π 1 - 2 À¬¥ ≈ß„π “√≈–≈“¬
              UK), °√–¥“…≈‘μ¡—  (Merck, Germany), magne-  μ—«Õ¬à“ß ª√‘¡“μ√ 2 ¡‘≈≈‘≈‘μ√  —߇°μ ’∑’ˇª≈’ˬπ
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