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                                                   Abstract

                 Physico-chemical specification of Bael fruit
                                , †
                 Apirak Sakpetch* , Duangpen Pattamadilok*, Sirikarn Thana-ariyaroj*,
                 Peradhama Thiemthieprat*, Sakwichai Ontong*, Nuchattra Chansuvanich*
                 *Medicinal Plant Research Institute, Department of Medical Sciences, Nonthaburi 11000
                 †
                  Corresponding author: Apirak.s@dmsc.mail.go.th

                     Study on physico-chemical characteristics of Bael fruits [Aegle marmelos (L.) Corrêa] family Rutaceae
                 aimed to obtain specification data to establish standard for chemical quality control for this kind of medicinal
                 plant. From 29 samples of Bael fruits were collected from natural sources and traditional drugstores during
                 June 2011 - April 2013. Chemical identifications using color reaction and thin layer chromatography and
                 evaluation of physico-chemical properties including contents of water, total ash, acid-insoluble ash, 95%etha-
                 nol extractive, 50%ethanol extractive and water extractive were performed. The percentage of these values
                 were calculated. Study on color reaction of ethanolic extract of Bael fruits exhibited positive result with
                 Liebermann Burchard, Shinodaûs and 1% FeCl . It also showed to clear the color of KMnO  solution. In
                                                   3                                 4
                 chemical identification by TLC technique, silica gel GF254 was used as stationary phase and mixture of
                 toluene and ether (3 : 2) was used as mobile phase. Spot of imperatorin could be observed at R value of 0.67.
                                                                                    f
                 Contents of water, total ash, acid-insoluble ash, 95%ethanol extractive, 50%ethanol extractive and water
                 extractive were (mean±sd) 8.05±1.44, 3.15±031, 0.15±0.11, 15.78±3.71, 41.36±6.58 and 49.93±5.71% w/w,
                 respectively. Standard for chemical quality control of Bael fruits should be established. Content of water
                 content, total ash and acid-insoluble ash content should be not more than 9, 3 and 1% w/w, respectively.
                 95%ethanol, 50% ethanol and water extractive values should be not less than 14, 37 and 45% w/w,
                 respectively. In chemical identification by TLC technique, imperatorin could be observed.

                     Key words:  Aegle marmelos, Rutaceae, Bael fruit, specification








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