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Abstract
Enhancement of Root Induction for Tissue Culture of Stemona collinsae Craib. and Transplanting
Monthar Wongmaneeroj*, Rongrong Homhual*, Siriwan Burikam**, Suratwadee Jiwajinda*
*Central Laboratory and Greenhouse Complex, Research and Development Institute at Kamphaengsaen, Kasetsart
University, Kamphaengsaen Campus, Nakhon Pathom 73140.
**Scientific Equipment Center, Research and Development Institute, Kasetsart University, Jatujak, Bangkok 10900.
Tissue culture techniques for Stemona collinsae Craib. can propagate a considerable amount of plantlets,
but the additional step of “root induction” is somewhat difficult. Therefore, the experiment in root induction
techniques was conducted by modifying some ingredients in basic Murashige and Skooge (MS) media. Ini-
tially, the plantlets grown in modified MS (1962) media [added sugar 30 mg/L and Benzyladenine (BA) 2 mg/
L] were regenerated; and root induction in various MS additives was later followed. The modifications of MS
media, such as adding 412.5 mg/L of NH 4 NO 3 , 2,375 or 3,850 mg/L of KNO 3 , 30 or 60 g/L of sugar, 1 or 2 g/L of
4-(indole-3-yl) butyric acid (IBA), were compared with two controlled formulas (MS with IBA 1 mg/L with 30
or 60 g/L of sugar). The results showed that plantlets grown in media with 412.5 mg/L of NH 4 NO 3 , 2375 mg/
L of KNO 3 , 60 g/L of sugar and 1 g/L of IBA had the highest percentage (100%) of root induction and survival
percentage (100%).
Key words: Stemona collinsae Craib., tissue culture, root induction, transplanting