Page 96 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 4 ฉบับที่ 1

94 «“√ “√°“√·æ∑¬å·ºπ‰∑¬·≈–·æ∑¬å∑“߇≈◊Õ° ªï∑’Ë Ù ©∫—∫∑’Ë Ò µÿ≈“§¡ ÚıÙ¯-¡°√“§¡ ÚıÙ˘

1
IAA ¢π“¥§«“¡‡¢â¡¢âπ 0.1 - 1 ¡°./≈. ‡¡◊ËՇ擖‡≈’Ȭ߇ªìπ‡«≈“ 1 ∂÷ß 1 / ‡¥◊Õπ ‚¥¬∑’ËŒÕ√å‚¡π IAA
2
1
¢π“¥§«“¡‡¢â¡¢âπ 0.1 ¡°./≈. ∑”„À⇪Õ√凴Áπµå°“√‡°‘¥√“° Ÿß∑’Ë ÿ¥ ‡¡◊ËՇ擖‡≈’È¬ß 1 - 1 / ‡¥◊Õπ
2
à«πŒÕ√å‚¡π IAA ¢π“¥§«“¡‡¢â¡¢âπ 1 ¡°./≈. ®–∑”„À⇰‘¥√“°·≈–µâπ∑’Ë ¡∫Ÿ√≥å∑’Ë ÿ¥ ‡¡◊ËÕ
1
‡æ“–‡≈’Ȭ߇ªìπ‡«≈“ 1 / ‡¥◊Õπ ŒÕ√å‚¡π IBA “¡“√∂∑”„À⇰‘¥√“°‰¥â¥’·µàµâπ®–‡µ’Ȭ°«à“ „∫‡≈Á°°«à“
2
°“√„™âŒÕ√å‚¡π IAA ŒÕ√å‚¡π NAA ∑”„À⇰‘¥√“°‰¥â·µàµâπ®–‡µ’Ȭ·≈–√“°‡°‘¥·§≈≈— µâπÕàÕπ∑’ˉ¥âπ”
¡“ª≈Ÿ°„π‡√◊Õπ‡æ“–™” ¡’‡ªÕ√凴Áπµå√Õ¥™’«‘µ‡©≈’ˬ 94.25 % ‡¡◊ËÕª≈Ÿ°‰¥â 1 ‡¥◊Õπ
°“√µ√«®«‘‡§√“–Àå “√ ”§—≠ total saponin „π¬Õ¥ÕàÕπªí≠®¢—π∏å “¬æ—π∏ÿå®’π·≈– “¬

æ—π∏ÿå‰∑¬ ∑’ˇ擖‡≈’Ȭß∫π Ÿµ√Õ“À“√ MS 58 ·≈– MS 58+C5 ‡ªìπ‡«≈“ 1 ‡¥◊Õπ ¡’§à“ total saponin
¢Õ߬եÕàÕπªí≠®¢—π∏å “¬æ—π∏ÿå®’π‡∑à“°—∫ 5.10 % ·≈– 5.08 % ·≈–¢Õ߬եÕàÕπªí≠®¢—π∏å “¬æ—π∏ÿå
‰∑¬‡∑à“°—∫ 5.21 % ·≈– 5.45 % µ“¡≈”¥—∫ µâπªí≠®¢—π∏å “¬æ—π∏ÿå‰∑¬∑’˪≈Ÿ°„π‡√◊Õπ‡æ“–™”‡ªìπ‡«≈“
3 ‡¥◊Õπ ¡’§à“ total saponin ‡∑à“°—∫ 6.67 %

°‘µµ‘°√√¡ª√–°“»

¢Õ¢Õ∫§ÿ≥ °√¡æ—≤π“°“√·æ∑¬å·ºπ‰∑¬·≈–°“√·æ∑¬å∑“߇≈◊Õ° ∑’Ë°√ÿ≥“„Àâ∑ÿπ π—∫ πÿπ
°“√«‘®—¬§√—Èßπ’È
¢Õ¢Õ∫§ÿ≥ §ÿ≥‡¬Á𮑵√ ‡µ™–¥”√ß ‘π ∑’Ë°√ÿ≥“ª√– “πß“π¥â“π°“√„Àâ∑ÿπ π—∫ πÿπ°“√«‘®—¬
·≈–„Àâµâπªí≠®¢—π∏å “¬æ—π∏ÿå®’π®“° ∂“∫—π°“√·æ∑¬å·ºπ‰∑¬ - ®’π ‡Õ‡´’¬µ–«—πÕÕ°‡©’¬ß„µâ

°√¡æ—≤π“°“√·æ∑¬å·ºπ‰∑¬·≈–°“√·æ∑¬å∑“߇≈◊Õ°
¢Õ¢Õ∫§ÿ≥ 𓬷æ∑¬å™«≈‘µ —𵑰‘®√ÿà߇√◊Õß ºŸâÕ”π«¬°“√ ∂“∫—π°“√·æ∑¬å‰∑¬ - ®’π
‡Õ‡´’¬µ–«—πÕÕ°‡©’¬ß„µâ ∑’Ë°√ÿ≥“Õ”π«¬§«“¡ –¥«°„π¥â“π°“√«‘®—¬·≈–¢Õ¢Õ∫§ÿ≥ §ÿ≥«“√ÿ≥’
®‘√«—≤π“æß»å »Ÿπ¬åµ√«® Õ∫·≈–√—∫√Õߧÿ≥¿“æ ¡ÿπ‰æ√ ∂“∫—π«‘®—¬ ¡ÿπ‰æ√ ∑’˙૬µ√«®«‘‡§√“–Àå

total saponin

‡Õ° “√Õâ“ßÕ‘ß
1. ∂“∫—π«‘®—¬ ¡ÿπ‰æ√ °√¡«‘∑¬“»“ µ√å°“√·æ∑¬å °√–∑√«ß “∏“√≥ ÿ¢ ¡ÿπ‰æ√πà“√Ÿâ (2) ªí≠®¢—π∏å
Gynostemma pentaphyllum (Thunb.) Makino æ‘¡æå∑’Ë‚√ßæ‘¡æå°“√»“ π“ æ‘¡æå§√—Èß∑’Ë 1
¡’π“§¡ æ.». 2548 Àπâ“ 1 - 104.

2. Li L , JiaoL , Lau BH. Protective effect of gypenosides against oxidative stress in
phagocytes , vascular endothelial cells and liver microsomes. Cancer Biother. 1993 ;
8 (3) : 263 - 272.
3. Ma Z , Yang Z. The scavenging effects of Astragalus and Gynostemma pentaphyllum

with its production on O - and OH. Zhong Yao Cai. 2003 ; 22 (6) : 303 - 306.
2
[ Abstract ]
4. Kawpinit D. The pharmacological activities of Gynostemma pentahyllum Makino. (Thesis)