Page 99 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 5 ฉบับที่ 2 (ฉบับเสริม)

๙๑

PO-11

§š›·Í˜oµœ°œ»¤¼¨°·­¦³ °Š­µ¦­„´—ĝ´ª®¨ªŠ (Nelumbo nucifera Gaertn.)

¤µ¦»˜˜´ÊŠª´•œµ»¨¸¡¦, ›´¨´„¬–r £¼¤·ª´•œ³
£µ‡ª·µª·š¥µ«µ­˜¦r„µ¦Â¡š¥r ‡–³ª·š¥µ«µ­˜¦r ¤®µª·š¥µ¨´¥¼¦¡µ

®¨´„„µ¦Â¨³Á®˜»Ÿ¨ °œ»¤¼¨°·­¦³ (free radical) Áž}œ­µ¦š¸ÉŪ˜n°„µ¦šÎµž’·„¦·¥µ„´­µ¦¸ªÃ¤Á¨„»¨Ánœ

Þ¦˜¸œÅ ¤´œÂ¨³ DNA š¸É°¥¼nĜ¦nµŠ„µ¥¤œ»¬¥r Ÿ¨‹µ„„µ¦šÎµž’·„¦·¥µœ¸Ê„n°Ä®oÁ„·—Ÿ¨Á­¸¥˜n°¦nµŠ„µ¥¤µ„¤µ¥

Ánœ„n°Ä®oÁ„·—懮´ªÄ‹Â¨³®¨°—Á¨º°— ‡ªµ¤‹ÎµÁ­ºÉ°¤ ¨³„µ¦„¨µ¥¡´œ›»r °Š­µ¦¡´œ›»„¦¦¤‹œ°µ‹¡´•œµ
Áž}œÃ¦‡¤³Á¦ÈŠ˜µ¤¤µÅ—o Ĝž{‹‹»´œœ´„ª·‹´¥¡¥µ¥µ¤‡oœ‡ªoµ®µ ¡ºŸ´„Ÿ¨Å¤o ¨³­¤»œÅ¡¦ Á¡ºÉ°Äo˜n°˜oµœ

°œ»¤¼¨°·­¦³Â¨³¨—°»´˜·„µ¦–r °ŠÃ¦‡š¸É¤¸‡ªµ¤­´¤¡´œ›r„´„µ¦¤¸°œ»¤¼¨°·­¦³š¸ÉÁ¡·É¤¤µ„ ¹Êœ´ª®¨ªŠÁž}œ¡º

­¤»œÅ¡¦œ·—®œ¹ÉŠš¸É¤¸‡»–­¤´˜·Äœ„µ¦˜oµœ°œ»¤¼¨°·­¦³ ˜n‹µ„„µ¦ššªœª¦¦–„¦¦¤Á„¸É¥ª„´§š›·Í °Š´ª
®¨ªŠš¸É­µ¤µ¦™¥´¥´ÊŠ„µ¦šÎµ¨µ¥ DNA ‹µ„°œ»¤¼¨°·­¦³ ¡ªnµ¥´Š¤¸ o°¤¼¨œo°¥¤µ„ —´Šœ´Êœ o°¤¼¨‹µ„Šµœª·‹´¥

œ¸Ê°µ‹œÎµÅžÄoÁž}œ o°¤¼¨¡ºÊœ“µœÄœ„µ¦¥ºœ¥´œ§š›·ÍšµŠÁ£­´ª·š¥µ ¨³°µ‹¤¸„µ¦¡´•œµ˜n°Åž‹œÅ—oÁž}œ¥µ

˜oµœ°œ»¤¼¨°·­¦³Äœ°œµ‡˜

ª´˜™»ž¦³­Š‡r Á¡ºÉ°®µ§š›·Í˜oµœ°œ»¤¼¨°·­¦³‹µ„ĝ´ª®¨ªŠš¸É­„´——oª¥—oª¥ Ö% °¨„°±°¨r

ª·›¸—εÁœ·œ„µ¦ ĝ´ª®¨ªŠ™¼„­„´—ץčo Ö % °¨„°±°¨r ¨oª‹¹ŠœÎµÅž„¦°ŠÁ¡ºÉ°Â¥„„µ„š·ÊŠ®¨´Š‹µ„œ´Êœ

œÎµÅž¦³Á®·—˜´ªšÎµ¨³¨µ¥°°„—oª¥Á‡¦ºÉ°Š lyophilizer Á¤ºÉ°Å—o­µ¦­„´—ĝ´ª®¨ªŠÂ¨oª‹¹ŠœÎµÅžš—­°
‡»–­¤´˜·Äœ„µ¦˜oµœ°œ»¤¼¨°·­¦³—oª¥ DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free radical ¨³

š—­°„µ¦žj°Š„´œ„µ¦šÎµ¨µ¥ plasmid DNA ‹µ„­µ¦ AAPH (2,2’-azobis (2-amidopropane)

dihydrochloride) ץčo gel electrophoresis š´ÊŠ­°Š„µ¦š—¨°ŠÄo ascorbic acid Ž¹ÉŠÁž}œ­µ¦˜oµœ°œ»¤¼¨
°·­¦³¤µ˜¦“µœÁž}œ˜´ªÁž¦¸¥Áš¸¥

Ÿ¨„µ¦«¹„¬µ ­µ¦­„´—ĝ´ª®¨ªŠÁ¤ºÉ°š—­°—oª¥­µ¦ DPPH ¡ªnµ ‡ªµ¤Á o¤ oœÒ, Ó.Ö, Ö, ÒÑ Â¨³ ÓÖ

ôg/ml ¤¸Áž°¦rÁŽœ˜r„µ¦¥´¥´ÊŠ°œ»¤¼¨°·­¦³ Ú, ÓÑ, ÕÒ, ØØ Â¨³ ÚÑ Áž°¦rÁŽÈœ˜r ˜µ¤¨Îµ—´Ã—¥¤¸‡nµ IC 50

Ášnµ„´×.Ô× ¤‡„./¤¨. Ÿ¨„µ¦š—­°‡»–­¤´˜·Äœ„µ¦žj°Š„´œ„µ¦šÎµ¨µ¥ plasmid DNA (supercoiled
form) ‹µ„­µ¦ AAPH ¡ªnµ ‡ªµ¤Á o¤ oœÖÑ-ÙÑÑ ôg/ml ­µ¤µ¦™¥´¥´ÊŠ„µ¦šÎµ¨µ¥ DNA ‹µ„­µ¦ AAPH

ŗo ×¥‡ªµ¤Á o¤ oœ °Š­µ¦­„´—ĝ´ª®¨ªŠš¸É­¼Š ¹Êœ­µ¤µ¦™¦´„¬µ supercoiled form ŗo¤µ„ ¹ÊœÂ¨³š¸É

‡ªµ¤Á o¤ oœ °Š­µ¦­„´—ĝ´ª®¨ªŠ ÙÑÑ ¤‡„./¤¨. ­µ¤µ¦™¥´¥´ÊŠ„µ¦šÎµ¨µ¥ plasmid DNA ‹µ„­µ¦
AAPH ŗo—¸„ªnµ ascorbic acid ‡ªµ¤Á o¤ oœÕÑôM °¥nµŠ¤¸œ´¥­Îµ‡´šµŠ­™·˜· (p<Ñ.ÑÖ)

o°­¦»ž ­µ¦­„´—ĝ´ª®¨ªŠ—oª¥ Ö% °¨„°±°¨r ­µ¤µ¦™˜oµœ°œ»¤¼¨°·­¦³‹µ„­µ¦ DPPH ¨³ AAPH ŗo
¨³ž¦·¤µ– °Š­µ¦­„´—ĝ´ª®¨ªŠš¸É¤µ„ ¹ÊœÄ®oŸ¨Äœ„µ¦˜oµœ°œ»¤¼¨°·­¦³š¸ÉÁ¡·É¤¤µ„ ¹Êœš´ÊŠ­°Š„µ¦š—¨°Š