Page 117 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 5 ฉบับที่ 2 (ฉบับเสริม)

๑๐๙

PO-28

„µ¦«¹„¬µ‡ªµ¤Áž}œ¡·¬ °Š­µ¦­„´—Þ¦˜¸œ‹µ„ÁœºÊ°Á¥ºÉ°­´˜ªr˜n°ÁŽ¨¨r¤³Á¦ÈŠ˜n°¤¨¼„®¤µ„œ·—

PZ-HPV-7

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Å¡¼¨¥r Á¦º°Š¡´•œ¡Š«r , »¨¸¦´˜œr ¦¦‹Š¨· ·˜„»¨ , ¤–¸¦´˜œr ¤¸¡¨°¥ , °œª´­»ª¦¦–„»¨ , šª¸«´„—·Í ­»œš¦›œ«µ­˜¦r Ò
Ò. iµ¥Á£­´Â¨³Ÿ¨·˜£´–”r›¦¦¤µ˜· ­™µ´œª·‹´¥ª·š¥µ«µ­˜¦r¨³Áš‡ÃœÃ¨¥¸Â®nŠž¦³Áš«Åš¥ (ªª) Áš‡Ãœ›µœ¸
Ó iµ¥Áš‡ÃœÃ¨¥¸®¨´Š„µ¦Á„ȝÁ„¸É¥ª ­™µ´œª·‹´¥ª·š¥µ«µ­˜¦r¨³Áš‡ÃœÃ¨¥¸Â®nŠž¦³Áš«Åš¥ (ªª) Áš‡Ãœ›µœ¸

ª´˜™»ž¦³­Š‡r Á¡ºÉ°«¹„¬µž¦³­·š›·£µ¡„µ¦°°„§š›·Í °Š­µ¦­„´—Þ¦˜¸œ‹µ„ÁœºÊ°Á¥ºÉ°­´˜ªrĜ„µ¦¥´¥´ÊŠ„µ¦

Á‹¦·Á˜·Ã˜ °ŠÁŽ¨¨r¤³Á¦ÈŠ˜n°¤¨¼„®¤µ„

ª´­—»Â¨³ª·›¸„µ¦ ­„´—­µ¦‹µ„ÁœºÊ°Á¥ºÉ°­´˜ªr­nªœ°´–”³Ã—¥ acid precipitation ‹µ„œ´ÊœœÎµÅžšÎµŸŠÂ®oŠ

(lyophilization) šÎµÄ®oŗo­µ¦­„´—Þ¦˜¸œ testis A (A; cytoplasmic fraction), testis B (B; nuclear fraction)

¨³ testis C (C; membrane fraction) œÎµ­µ¦­„´—š´ÊŠ­µ¤­nªœ¤µš—­°„µ¦¥´¥´ÊŠ„µ¦ÂnŠ˜´ª °ŠÁŽ¨¨r
¤³Á¦ÈŠ˜n°¤¨¼„®¤µ„œ·— PZ-HPV-7 š¸Éŗo¦´„µ¦ transfection ץŪ¦´­ HPV Ä®o„¨µ¥Áž}œÁŽ¨¨r¤³Á¦ÈŠ

š—­°‡ªµ¤Áž}œ¡·¬ °Š­µ¦­„´—×¥ Ò. ­´ŠÁ„˜¦¼ž¦nµŠ °ŠÁŽ¨¨rš¸ÉÁž¨¸É¥œÅž (cell morphology) Ó. «¹„¬µ

„µ¦šÎµŠµœ °ŠÁ°œÅŽ¤rŤØ‡°œÁ—¦¸¥Â¨³ Ô. ª´—ž¦·¤µ–—¸Á°ÈœÁ°Áš¸¥„´„¨»n¤‡ª‡»¤

Ÿ¨„µ¦«¹„¬µ ­µ¦­„´—Þ¦˜¸œ‹µ„ testis C š¸Éŗo‹µ„­nªœ °Š membrane ¤¸Ÿ¨šÎµÄ®oÁŽ¨¨r¤³Á¦ÈŠ˜n°¤¨¼„®¤µ„

¤¸¦¼ž¦nµŠš¸ÉÁž¨¸É¥œÂž¨ŠÅž ¨³Á¤ºÉ°š—­°‡ªµ¤Áž}œ¡·¬ °Š­µ¦­„´— (cytotoxicity) š´ÊŠª·›¸ MTT ¨³

Cyquant Assay ¡ªnµ testis C ¤¸‡»–­¤´˜·Äœ„µ¦¥´¥´ÊŠ„µ¦ÂnŠ˜´ª °ŠÁŽ¨¨r¤³Á¦ÈŠ˜n°¤¨¼„®¤µ„ ×¥¤¸‡nµ
IC Ášnµ„´Ô.Ñ rÑ.Õ Å¤Ã‡¦„¦´¤Ãž¦˜¸œ/¤·¨¨·¨·˜¦
50
o°­¦»ž ­µ¦­„´—Þ¦˜¸œ‹µ„ÁœºÊ°Á¥ºÉ°­´˜ªr­nªœ testis C (membrane fraction) ­µ¤µ¦™¥´¥´ÊŠ„µ¦ÂnŠ˜´ª °Š

ÁŽ¨¨r¤³Á¦ÈŠ˜n°¤¨¼„®¤µ„ ×¥„¨Å„Ÿnµœ„µ¦¥´¥´ÊŠ„µ¦šÎµŠµœ °ŠÁ°œÅŽ¤rĜŤØ‡°œÁ—¦¸¥